Ebook: Peptides as Probes in Muscle Research

Protein-protein interactions are involved in muscle contraction and signal transduction. This book describes how synthetic peptides may be used, much like antibodies, both as specific inhibitors and as molecular probes to explore the cognitive interfaces between interacting proteins and their functional significance. This offers the prospect of very selective intervention in cellular mechanisms. These timely contributions by several experts will appeal to the researchers in muscle physiology, cardiovascular pharmacology and cell biology who are interested in this new approach.

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Protein-protein interactions are involved in muscle contraction and signal transduction. This book describes how synthetic peptides may be used, much like antibodies, both as specific inhibitors and as molecular probes to explore the cognitive interfaces between interacting proteins and their functional significance. This offers the prospect of very selective intervention in cellular mechanisms. These timely contributions by several experts will appeal to the researchers in muscle physiology, cardiovascular pharmacology and cell biology who are interested in this new approach.

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Protein-protein interactions are involved in muscle contraction and signal transduction. This book describes how synthetic peptides may be used, much like antibodies, both as specific inhibitors and as molecular probes to explore the cognitive interfaces between interacting proteins and their functional significance. This offers the prospect of very selective intervention in cellular mechanisms. These timely contributions by several experts will appeal to the researchers in muscle physiology, cardiovascular pharmacology and cell biology who are interested in this new approach.
Content:
Front Matter....Pages I-X
Introduction....Pages 1-5
Structure of Actin....Pages 7-14
Interaction of Actin 1–28 with Myosin and Troponin I and the Importance of These Interactions to Muscle Regulation....Pages 15-31
Probing Myosin Head Structure with Monoclonal Antibodies and Recombinant Technology....Pages 33-37
An Actin-Binding Site on Myosin....Pages 39-48
Competitive Inhibition of Maximum Ca-Activated Force in Skinned Muscle Fibers by Cationic Peptides from the SH-1 Region of Myosin Heavy Chain....Pages 49-55
The Use of Peptide Mimetics to Define the Actin-Binding Sites on the Head of the Myosin Molecule....Pages 57-68
Interference of Myosin Peptides with Weak and Strong Actin Interaction of Cross-Bridges in Skeletal Muscle Fibres....Pages 69-80
Caldesmon Derived Polypeptides as Probes of Force Production in Skeletal Muscle....Pages 81-93
Myosin and Troponin Peptides Affect Calcium Sensitivity of Skinned Muscle Fibres....Pages 95-109
Peptides as Probes of the Mechanisms Regulating Smooth Muscle Contractility: Studies on Skinned Fibres....Pages 111-117
Antibodies as Probes of the Mechanisms Regulating Smooth Muscle Contractility: Studies on Skinned Fibres....Pages 119-129
Regulation of Ca2+ Release from Sarcoplasmic Reticulum of Skeletal Muscle by an Endogenous Substance....Pages 131-140
Defining Sites and Mechanisms of Interaction Between Rhodopsin and Transducin....Pages 141-149
Comparative Studies on Chicken Skeletal and Smooth Muscle Dystrophins....Pages 151-159
Use of Synthetic Peptides in the Study of the function of Dystrophin....Pages 161-170
Back Matter....Pages 171-174

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Protein-protein interactions are involved in muscle contraction and signal transduction. This book describes how synthetic peptides may be used, much like antibodies, both as specific inhibitors and as molecular probes to explore the cognitive interfaces between interacting proteins and their functional significance. This offers the prospect of very selective intervention in cellular mechanisms. These timely contributions by several experts will appeal to the researchers in muscle physiology, cardiovascular pharmacology and cell biology who are interested in this new approach.
Content:
Front Matter....Pages I-X
Introduction....Pages 1-5
Structure of Actin....Pages 7-14
Interaction of Actin 1–28 with Myosin and Troponin I and the Importance of These Interactions to Muscle Regulation....Pages 15-31
Probing Myosin Head Structure with Monoclonal Antibodies and Recombinant Technology....Pages 33-37
An Actin-Binding Site on Myosin....Pages 39-48
Competitive Inhibition of Maximum Ca-Activated Force in Skinned Muscle Fibers by Cationic Peptides from the SH-1 Region of Myosin Heavy Chain....Pages 49-55
The Use of Peptide Mimetics to Define the Actin-Binding Sites on the Head of the Myosin Molecule....Pages 57-68
Interference of Myosin Peptides with Weak and Strong Actin Interaction of Cross-Bridges in Skeletal Muscle Fibres....Pages 69-80
Caldesmon Derived Polypeptides as Probes of Force Production in Skeletal Muscle....Pages 81-93
Myosin and Troponin Peptides Affect Calcium Sensitivity of Skinned Muscle Fibres....Pages 95-109
Peptides as Probes of the Mechanisms Regulating Smooth Muscle Contractility: Studies on Skinned Fibres....Pages 111-117
Antibodies as Probes of the Mechanisms Regulating Smooth Muscle Contractility: Studies on Skinned Fibres....Pages 119-129
Regulation of Ca2+ Release from Sarcoplasmic Reticulum of Skeletal Muscle by an Endogenous Substance....Pages 131-140
Defining Sites and Mechanisms of Interaction Between Rhodopsin and Transducin....Pages 141-149
Comparative Studies on Chicken Skeletal and Smooth Muscle Dystrophins....Pages 151-159
Use of Synthetic Peptides in the Study of the function of Dystrophin....Pages 161-170
Back Matter....Pages 171-174
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