Ebook: Enzymology and Molecular Biology of Carbonyl Metabolism 3

The Fifth International Workshop on the Enzymology and Molecular Biology of Carbonyl Metabolism was held at Purdue University in June, 1990. This represents the fifth time that I had the privilege of organizing the scientific program. It was the first time that I actually hosted the meeting. I wish to salute my four previous co-organizers and the thousands of scientists who have hosted other meetings. It is much easier to arrange the scientific program and edit the proceedings. No local organization could occur without the help of ones research group and, in this case, my wife. I sincerely thank Esther and my research group for their advise and help. At this Workshop, similar to the preceeding ones, much new information was presented. It was apparent how molecular biological techniques were influencing the direction of the research on the three families of enzymes discussed. It also was apparent that not all biochemical problems could be solved by using these techniques. Many of the presentations showed how important advances still could be made using more traditional biochemical approaches.

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Content:
Front Matter....Pages i-ix
Aldehyde Dehydrogenases: What can be Learned from a Baker’s Dozen Sequences?....Pages 1-8
Rat Class 3 Aldehyde Dehydrogenase: Crystals and Preliminary Analysis....Pages 9-11
Probing the Active Site of Aldehyde Dehydrogenase by Site Directed Mutagenesis....Pages 13-17
Localization of Cysteine 302 at the Active Site of Aldehyde Dehydrogenase....Pages 19-30
PH Effects on Cytoplasmic Aldehyde Dehydrogenase from Sheep Liver....Pages 31-41
Biochemical, Immunological, and Molecular Characterization of a “High KM” Aldehyde Dehydrogenase....Pages 43-52
Purification and Properties of Baboon Corneal Aldehyde Dehydrogenase: Proposed UVR Protective Role....Pages 53-60
A Polymorphism in the Rat Liver Mitochondrial ALDH2 Gene is Associated with Alcohol Drinking Behavior....Pages 61-65
Aldehydic Products of Lipid Peroxidation: Substrates or Inhibitors of Hepatic Aldehyde Dehydrogenase?....Pages 67-73
Metabolism of 4-Hydroxynonenal in Hepatoma Cell Lines....Pages 75-84
Role of Aldehyde Dehydrogenase (ALDH) in the Detoxication of Cyclophosphamide (CP) in Rat Embryos....Pages 85-95
Human and Mouse Hepatic Aldehyde Dehydrogenases Important in the Biotransformation of Cyclophosphamide and the Retinoids....Pages 97-104
A Paradigm for Aldehyde Oxidation: Histidinol Dehydrogenase....Pages 105-112
Structural Studies of Pig Lens Aldose Reductase: Reversible Dimerization of the Enzyme....Pages 113-118
cDNA Cloning and Expression of Human Aldose Reductase....Pages 119-127
Osmotic Stress Induces Aldose Reductase in Glomerular Endothelial Cells....Pages 129-138
Purification of Aldose and Aldehyde Reductases from Dog Kidney....Pages 139-152
Study on Dihydrodiol Dehydrogenase (I) Molecular Forms of the Enzyme and the Presence of a Dihydrodiol Specific Enzyme in Bovine Liver Cytosol....Pages 153-163
Study on Dihydrodiol Dehydrogenase (II) Modulation of Dihydrodiol Dehydrogenase Activity by Biological Disulfides....Pages 165-175
Distribution of Dimeric Dihydrodiol Dehydrogenase in Pig Tissues and its Role in Carbonyl Metabolism....Pages 177-185
Inhibition of Aldehyde Reductase by Carboxylic Acids....Pages 187-196
Reactivity of Enzyme Modification Reagents with Aldose Reductase and Aldehyde Reductase....Pages 197-204
A Kinetic Perspective on the Peculiarity of Aldose Reductase....Pages 205-216
Best-Fit Analysis of Kinetic Scheme for the Stepwise Reduction of the “Diketo” Group of 6-Pyruvoyl Tetrahydropterin by Sepiapterin Reductase....Pages 217-228
Catalysis by Yeast Alcohol Dehydrogenase....Pages 229-239
Alcohol Dehydrogenase Gene Expression and Cloning of the Mouse ?-Like ADH....Pages 241-251
Role of Alcohol Dehydrogenase Polymorphism in Ethanol Metabolism and Alcohol-Related Diseases....Pages 253-262
Characterization of Human Alcohol Dehydrogenases Containing Substitutions at Amino Acids 47 and 51....Pages 263-270
Endocrine Regulation and Methylation Patterns of Rat Class I Alcohol Dehydrogenase in Liver and Kidney....Pages 271-275
Mammalian Class II Alcohol Dehydrogenase: Species and Class Comparisons at Genomic and Protein Levels....Pages 277-284
The Activation of Alcohols by Liver Alcohol Dehydrogenase: Dependence of Inhibition Upon the pKa Lowering Effect....Pages 285-292
Glutathione-Dependent Formaldehyde Dehydrogenase (EC 1.2.1.1): Evidence for the Identity with Class III Alcohol Dehydrogenase....Pages 293-304
Kinetics and Mechanism of Methanol and Formaldehyde Interconversion and Formaldehyde Oxidation Catalyzed by Liver Alcohol Dehydrogenase....Pages 305-313
Novel Substrates and Inhibitors of Human Liver Sorbitol Dehydrogenase....Pages 315-325
Comparison of the Primary Structures of NAD(P)-Dependent Bacterial Alcohol Dehydrogenases....Pages 327-336
Back Matter....Pages 337-345
....Pages 347-359