Ebook: Photobiological Techniques

The fIrst edition of the Science 0/ Photobiology edited by Kendric C. Smith (plenum Press, 1977) was a comprehensive textbook of photobiology, devoting a chapter to each of the subdisciplines of the fIeld. At the end of many of these chapters there were brief descriptions of simple experiments that students could perform to demonstrate the principles discussed. In the succeeding years some photobiologists felt that a more complete publica­ tion of experiments in photobiology would be a useful teaching tool. Thus, in the 1980s the American Society for Photobiology (ASP) attempted to produce a laboratory manual in photobiology. Cognizant of these efforts, Kendric Smith elected to publish the second edition of The Science o/Photobiology (1989) without experiments; anticipating the comple­ tion of the ASP laboratory manual. Unfortunately, the initial ASP efforts met with limited success, and several years were to pass before a photobiology laboratory manual became a reality. One of the major stumbling blocks to production of an accurate and reliable laboratory manual was the requirement that the experiments be tested, not just by the author who is familiar with the techniques, but by students who may be quite new to photobiology. How could this be accomplished with limited resources? Many ideas were considered and discarded, before a workable solution was found. The catalyst that enabled the careful screening of all experiments in this book was a NATO Advanced Study Institute (ASI) devoted entirely to this purpose.

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Photophysics: The Nature of the Light Field in Biological Media B.C.Wilson.Photochemistry: Quantum Yield of a Photochemical Reaction; R.H. Potter, et al.Photosensitization: Photosensitization: Reaction Pathways; J.E. van Lier.Photosynthesis: Near Infra-Red Flash Absorption: A Tool for Studying Photosynthesis; P. Mathis, et al.UVEffects: Response to Ultraviolet Radiation in a Simple Eukaryote; (Yeast): Genetic Control and Biological Consequences; D. Averbeck, etal.Environmental Photobiology: Isolation and Biological Consequences of Plant Derived Phototoxins; J.T. Arnason, et al.Vision: Visual Pigments: Absorbance Spectra and Photoproducts; J.K. Bowmaker.Photomorphogenesis: Photomorphogenesis Responses to UV Light: Involvement of Phytochrome and Photoreceptors; B. Lercari.Photomovement: Photomovement; W. Nultsch, et al.Bioluminescence: Bioluminescence: Biochemistry for Fun and Profit; J. Lee.Photomedicine: Photomedicine: Photodermatology B. Johnson.OtherExperiments: Experiments Reprinted from The Science of Photobiology, First Edition; K.C. Smith.Appendix: Units of Measure and Conversion Factors. 62 additional articles. Index.

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Photophysics: The Nature of the Light Field in Biological Media B.C.Wilson.Photochemistry: Quantum Yield of a Photochemical Reaction; R.H. Potter, et al.Photosensitization: Photosensitization: Reaction Pathways; J.E. van Lier.Photosynthesis: Near Infra-Red Flash Absorption: A Tool for Studying Photosynthesis; P. Mathis, et al.UVEffects: Response to Ultraviolet Radiation in a Simple Eukaryote; (Yeast): Genetic Control and Biological Consequences; D. Averbeck, etal.Environmental Photobiology: Isolation and Biological Consequences of Plant Derived Phototoxins; J.T. Arnason, et al.Vision: Visual Pigments: Absorbance Spectra and Photoproducts; J.K. Bowmaker.Photomorphogenesis: Photomorphogenesis Responses to UV Light: Involvement of Phytochrome and Photoreceptors; B. Lercari.Photomovement: Photomovement; W. Nultsch, et al.Bioluminescence: Bioluminescence: Biochemistry for Fun and Profit; J. Lee.Photomedicine: Photomedicine: Photodermatology B. Johnson.OtherExperiments: Experiments Reprinted from The Science of Photobiology, First Edition; K.C. Smith.Appendix: Units of Measure and Conversion Factors. 62 additional articles. Index.
Content:
Front Matter....Pages i-xiv
The Nature of the Light Field in Biological Media....Pages 1-18
Measurement of Fluoresecence Decays....Pages 19-35
A Non-Invasive Technique for in Vivo Pharmacokinetic Measurements Using Optical Fiber Spectrofluorimetry....Pages 37-43
Quantum Yield of a Photochemical Reaction....Pages 45-57
Photochemistry and Photobiology of Tetrapyrrole Pigments....Pages 59-76
The Flavin-EDTA System in the Study of Photocatalytic Redox Reactions....Pages 77-84
Photosensitization: Reaction Pathways....Pages 85-98
Membrane Photomodification....Pages 99-115
Spectroscopic Determination of Hematoporphyrin-Membrane Partition Parameters....Pages 117-126
Near Infra-Red Flash Absorption: A Tool for Studying Photosynthesis....Pages 127-145
Chlorophyll Fluorescence Transients in Chloroplasts and Leaves....Pages 147-163
Response to Ultraviolet Radiation in a Simple Eukaryote (Yeast): Genetic Control and Biological Consequences....Pages 165-186
Isolation and Biological Activity of Plant Derived Phototoxins....Pages 187-196
Visual Pigments: Absorbance Spectra and Photoproducts....Pages 197-212
Measurement of Retinal Function Using the Electroretinogram....Pages 213-230
Photomorphogenic Responses to UV Light: Involvement of Phytochrome and UV Photoreceptors....Pages 231-248
Photomovement....Pages 249-277
Effects of Light on Circadian Rhythms in Plants and Animals....Pages 279-295
Bioluminescence: Biochemistry for Fun and Profit....Pages 297-321
Photomedicine: Photodermatology....Pages 323-346
Back Matter....Pages 363-381
Experiments Reprinted from The Science of Photobiology, First Edition....Pages 347-361

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Photophysics: The Nature of the Light Field in Biological Media B.C.Wilson.Photochemistry: Quantum Yield of a Photochemical Reaction; R.H. Potter, et al.Photosensitization: Photosensitization: Reaction Pathways; J.E. van Lier.Photosynthesis: Near Infra-Red Flash Absorption: A Tool for Studying Photosynthesis; P. Mathis, et al.UVEffects: Response to Ultraviolet Radiation in a Simple Eukaryote; (Yeast): Genetic Control and Biological Consequences; D. Averbeck, etal.Environmental Photobiology: Isolation and Biological Consequences of Plant Derived Phototoxins; J.T. Arnason, et al.Vision: Visual Pigments: Absorbance Spectra and Photoproducts; J.K. Bowmaker.Photomorphogenesis: Photomorphogenesis Responses to UV Light: Involvement of Phytochrome and Photoreceptors; B. Lercari.Photomovement: Photomovement; W. Nultsch, et al.Bioluminescence: Bioluminescence: Biochemistry for Fun and Profit; J. Lee.Photomedicine: Photomedicine: Photodermatology B. Johnson.OtherExperiments: Experiments Reprinted from The Science of Photobiology, First Edition; K.C. Smith.Appendix: Units of Measure and Conversion Factors. 62 additional articles. Index.
Content:
Front Matter....Pages i-xiv
The Nature of the Light Field in Biological Media....Pages 1-18
Measurement of Fluoresecence Decays....Pages 19-35
A Non-Invasive Technique for in Vivo Pharmacokinetic Measurements Using Optical Fiber Spectrofluorimetry....Pages 37-43
Quantum Yield of a Photochemical Reaction....Pages 45-57
Photochemistry and Photobiology of Tetrapyrrole Pigments....Pages 59-76
The Flavin-EDTA System in the Study of Photocatalytic Redox Reactions....Pages 77-84
Photosensitization: Reaction Pathways....Pages 85-98
Membrane Photomodification....Pages 99-115
Spectroscopic Determination of Hematoporphyrin-Membrane Partition Parameters....Pages 117-126
Near Infra-Red Flash Absorption: A Tool for Studying Photosynthesis....Pages 127-145
Chlorophyll Fluorescence Transients in Chloroplasts and Leaves....Pages 147-163
Response to Ultraviolet Radiation in a Simple Eukaryote (Yeast): Genetic Control and Biological Consequences....Pages 165-186
Isolation and Biological Activity of Plant Derived Phototoxins....Pages 187-196
Visual Pigments: Absorbance Spectra and Photoproducts....Pages 197-212
Measurement of Retinal Function Using the Electroretinogram....Pages 213-230
Photomorphogenic Responses to UV Light: Involvement of Phytochrome and UV Photoreceptors....Pages 231-248
Photomovement....Pages 249-277
Effects of Light on Circadian Rhythms in Plants and Animals....Pages 279-295
Bioluminescence: Biochemistry for Fun and Profit....Pages 297-321
Photomedicine: Photodermatology....Pages 323-346
Back Matter....Pages 363-381
Experiments Reprinted from The Science of Photobiology, First Edition....Pages 347-361
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