Ebook: Epithelia: Advances in Cell Physiology and Cell Culture

Epithelial cells probably constitute the most diverse group of cells found in the body. In addition to serving as interfaces between external and internal environments, their functions include ion and fluid secretion and reabsorp­ tion, protein exocytosis, hormone secretion, recognition, surface protection and the control of ciliary movement. By their very exposure on the surfaces of the body, epithelial cells are subjected to wide-ranging assault, by micro­ organisms and by chemical and physical forces. They are the targets for abrasion, infection and malignant transformation. Some epithelial cells show altered behaviour in inherited syndromes, such as cystic fibrosis, characterized by serious pancreatic and pulmonary disease. In view of the importance of epithelia and the fact that their function can be altered by environmental and inherited factors, they are the subject of intensive research, particularly so in the case of cancer where most tumours are of epithelial origin. The use of animal tissues in epithelial research continues to provide important advances and this, coupled with the need to focus more on human tissues, has prompted a greater research emphasis on accessible human epithelia and on the establishment of cell cultures from animal and human sources. For primary cell cultures and cell lines to be of value, they need to express properties appropriate to their progenitors and relevant to the study in progress.

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Content:
Front Matter....Pages i-x
Front Matter....Pages 1-1
Introduction....Pages 3-4
Gastrointestinal Epithelial Barrier to Acid: Studies with Isolated Membrane Vesicles and Cultured Epithelial Cells....Pages 5-27
Establishment and Characteristics of Human Colorectal Adenocarcinoma Cell Lines....Pages 29-47
Transepithelial Ion Transport in Cultured Colonic Epithelial Cell Monolayers....Pages 49-64
Front Matter....Pages 65-65
Introduction....Pages 67-67
Pancreatic Ducts: Isolation, Culture and Bicarbonate Transport....Pages 69-97
Cultured Epithelial Cells Derived from Human Fetal Pancreatic Duct....Pages 99-115
Front Matter....Pages 117-117
Introduction....Pages 119-120
Electrophysiology of the Nephron: New Insights gained from the Patch-clamp Technique....Pages 121-144
Transport Physiology of Renal and Intestinal Cell Culture Models....Pages 145-171
Established Renal Epithelial Cell Lines: Experimental Panacea or Artifact?....Pages 173-190
Front Matter....Pages 191-191
Introduction....Pages 193-194
Form and Function of Mammalian Airway Epithelia....Pages 195-220
Physiological Properties of Cultured Airway Epithelial Cells: Comparison with Intact Epithelial Tissues....Pages 221-232
Solution Transport Across Alveolar Epithelia of Fetal and Adult Lungs....Pages 233-253
Regulation of Ion Channels in Cultured Airway Epithelial Cells....Pages 255-283
Front Matter....Pages 285-285
Introduction....Pages 287-288
Isolated Human Skin Glands and Appendages: Models for Cystic Fibrosis, Acne Vulgaris, Alopecia and Hidradenitis Suppurativa....Pages 289-304
Electrophysiological and Morphological Studies on Secretory and Reabsorptive Segments of the Human Eccrine Sweat Gland and on Primary Cell Cultures Established from these Regions....Pages 305-332
Cell Culture Systems for the Study of Human Skin and Skin Glands....Pages 333-350
Back Matter....Pages 351-358

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Content:
Front Matter....Pages i-x
Front Matter....Pages 1-1
Introduction....Pages 3-4
Gastrointestinal Epithelial Barrier to Acid: Studies with Isolated Membrane Vesicles and Cultured Epithelial Cells....Pages 5-27
Establishment and Characteristics of Human Colorectal Adenocarcinoma Cell Lines....Pages 29-47
Transepithelial Ion Transport in Cultured Colonic Epithelial Cell Monolayers....Pages 49-64
Front Matter....Pages 65-65
Introduction....Pages 67-67
Pancreatic Ducts: Isolation, Culture and Bicarbonate Transport....Pages 69-97
Cultured Epithelial Cells Derived from Human Fetal Pancreatic Duct....Pages 99-115
Front Matter....Pages 117-117
Introduction....Pages 119-120
Electrophysiology of the Nephron: New Insights gained from the Patch-clamp Technique....Pages 121-144
Transport Physiology of Renal and Intestinal Cell Culture Models....Pages 145-171
Established Renal Epithelial Cell Lines: Experimental Panacea or Artifact?....Pages 173-190
Front Matter....Pages 191-191
Introduction....Pages 193-194
Form and Function of Mammalian Airway Epithelia....Pages 195-220
Physiological Properties of Cultured Airway Epithelial Cells: Comparison with Intact Epithelial Tissues....Pages 221-232
Solution Transport Across Alveolar Epithelia of Fetal and Adult Lungs....Pages 233-253
Regulation of Ion Channels in Cultured Airway Epithelial Cells....Pages 255-283
Front Matter....Pages 285-285
Introduction....Pages 287-288
Isolated Human Skin Glands and Appendages: Models for Cystic Fibrosis, Acne Vulgaris, Alopecia and Hidradenitis Suppurativa....Pages 289-304
Electrophysiological and Morphological Studies on Secretory and Reabsorptive Segments of the Human Eccrine Sweat Gland and on Primary Cell Cultures Established from these Regions....Pages 305-332
Cell Culture Systems for the Study of Human Skin and Skin Glands....Pages 333-350
Back Matter....Pages 351-358
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