Ebook: Cell Culture Techniques in Heart and Vessel Research

In the cardiovascular sciences, an increasing demand for the use of modern methods of cell biology has developed. The use of specific cell culture models of the various tissues involved is essential for most of these novel approaches. This book meets the demand for acomprehensive and easy accessible source for cell cul- ture methods in cardiovascular research as it was not previously available. The basic methods for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), smooth muscle cells and pericytes are described in detail by an international selection of experts. Special chapters discuss the use of growth factors and attachment substrates, techniques for co-cultures, cultures on permeable filter membranes and microcarrier cultures. The methodological descriptions are sufficiently detailed for an immediate application in the laboratory. All chapters also contain a critical evaluation of alternative approaches.

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In the cardiovascular sciences, an increasing demand for the use of modern methods of cell biology has developed. The use of specific cell culture models of the various tissues involved is essential for most of these novel approaches. This book meets the demand for acomprehensive and easy accessible source for cell cul- ture methods in cardiovascular research as it was not previously available. The basic methods for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), smooth muscle cells and pericytes are described in detail by an international selection of experts. Special chapters discuss the use of growth factors and attachment substrates, techniques for co-cultures, cultures on permeable filter membranes and microcarrier cultures. The methodological descriptions are sufficiently detailed for an immediate application in the laboratory. All chapters also contain a critical evaluation of alternative approaches.

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In the cardiovascular sciences, an increasing demand for the use of modern methods of cell biology has developed. The use of specific cell culture models of the various tissues involved is essential for most of these novel approaches. This book meets the demand for acomprehensive and easy accessible source for cell cul- ture methods in cardiovascular research as it was not previously available. The basic methods for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), smooth muscle cells and pericytes are described in detail by an international selection of experts. Special chapters discuss the use of growth factors and attachment substrates, techniques for co-cultures, cultures on permeable filter membranes and microcarrier cultures. The methodological descriptions are sufficiently detailed for an immediate application in the laboratory. All chapters also contain a critical evaluation of alternative approaches.
Content:
Front Matter....Pages I-XIII
Front Matter....Pages 1-1
Embryonic Chick Heart Muscle Cells....Pages 3-19
Neonatal Rat Heart Muscle Cells....Pages 20-35
Adult Ventricular Rat Heart Muscle Cells....Pages 36-60
Adult Atrial Cardiocytes in Culture....Pages 61-74
Muscle Cell Cultures from Human Heart....Pages 75-98
Attachment Substrates for Heart Muscle Cells....Pages 99-107
Microcarrier Culture of Neonatal Heart Cells....Pages 108-114
Front Matter....Pages 115-115
Macrovascular Endothelial Cells from Porcine Aorta....Pages 117-129
Microvascular Endothelial Cells from the Lungs....Pages 130-139
Microvascular Endothelial Cells from Brain....Pages 140-157
Microvascular Endothelial Cells from Heart....Pages 158-177
Macro- and Microvascular Endothelial Cells from Human Tissues....Pages 178-204
Vascular Endothelial Cell-Synthesized Extracellular Matrices as Attachment Substrates In Vitro....Pages 205-211
Endothelial Cells Grown on Filter Membranes....Pages 212-219
Microcarrier Cultures of Endothelial Cells....Pages 220-229
Growth Factors for Vascular Endothelial Cells....Pages 230-244
Front Matter....Pages 245-245
Smooth Muscle Cells from Rabbit Aorta....Pages 247-270
Smooth Muscle Cells from Adult Human Aorta....Pages 271-289
Coculture of Endothelial and Smooth Muscle Cells....Pages 290-298
Culture and Study of Pericytes....Pages 299-314
Front Matter....Pages 245-245
Attachment Substrates for Smooth Muscle Cells....Pages 315-333
Methods of Culturing Vascular Smooth Muscle Cells on Microcarriers....Pages 334-342
Growth Factors for Smooth Muscle Cells....Pages 343-350
Back Matter....Pages 351-362

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In the cardiovascular sciences, an increasing demand for the use of modern methods of cell biology has developed. The use of specific cell culture models of the various tissues involved is essential for most of these novel approaches. This book meets the demand for acomprehensive and easy accessible source for cell cul- ture methods in cardiovascular research as it was not previously available. The basic methods for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), smooth muscle cells and pericytes are described in detail by an international selection of experts. Special chapters discuss the use of growth factors and attachment substrates, techniques for co-cultures, cultures on permeable filter membranes and microcarrier cultures. The methodological descriptions are sufficiently detailed for an immediate application in the laboratory. All chapters also contain a critical evaluation of alternative approaches.
Content:
Front Matter....Pages I-XIII
Front Matter....Pages 1-1
Embryonic Chick Heart Muscle Cells....Pages 3-19
Neonatal Rat Heart Muscle Cells....Pages 20-35
Adult Ventricular Rat Heart Muscle Cells....Pages 36-60
Adult Atrial Cardiocytes in Culture....Pages 61-74
Muscle Cell Cultures from Human Heart....Pages 75-98
Attachment Substrates for Heart Muscle Cells....Pages 99-107
Microcarrier Culture of Neonatal Heart Cells....Pages 108-114
Front Matter....Pages 115-115
Macrovascular Endothelial Cells from Porcine Aorta....Pages 117-129
Microvascular Endothelial Cells from the Lungs....Pages 130-139
Microvascular Endothelial Cells from Brain....Pages 140-157
Microvascular Endothelial Cells from Heart....Pages 158-177
Macro- and Microvascular Endothelial Cells from Human Tissues....Pages 178-204
Vascular Endothelial Cell-Synthesized Extracellular Matrices as Attachment Substrates In Vitro....Pages 205-211
Endothelial Cells Grown on Filter Membranes....Pages 212-219
Microcarrier Cultures of Endothelial Cells....Pages 220-229
Growth Factors for Vascular Endothelial Cells....Pages 230-244
Front Matter....Pages 245-245
Smooth Muscle Cells from Rabbit Aorta....Pages 247-270
Smooth Muscle Cells from Adult Human Aorta....Pages 271-289
Coculture of Endothelial and Smooth Muscle Cells....Pages 290-298
Culture and Study of Pericytes....Pages 299-314
Front Matter....Pages 245-245
Attachment Substrates for Smooth Muscle Cells....Pages 315-333
Methods of Culturing Vascular Smooth Muscle Cells on Microcarriers....Pages 334-342
Growth Factors for Smooth Muscle Cells....Pages 343-350
Back Matter....Pages 351-362
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