Ebook: Calcium Regulating Hormones, Vitamin D Metabolites, and Cyclic AMP Assays and Their Clinical Application

Calcium metabolism is regulated by three specific hormones: parathor- mone, calciferol metabolites, and calcitonin. Cyclic AMP also plays an important part in calcium regulation, and its concentration in urine can be taken as a measure of parathyroid function. Methods for quantitative measurement of, e.g. calcium-regulating hormones and vitamin D metabolites as well as of cAMP are absolutely necessary for safe differential diagnosis of diseases of the parathyroid like hypo- and hyperparathyroidism. In this monograph all presently available methods are summarized as to whether they can already be obtained as test kits or are still being tested. Radioimmunoassays for the various peptide fragments such as 53-84, 44-68, 28-48, and 1-34 are described, as are immunochemiluminescence methods and adenylate cyclase bioassays. The same is true for the vitamin D metabolites, where the various assays for calcidiol and calcitriol such as HPLC, protein-binding assay with second antibody separation, and double antibody RIA are de§ scribed. Finally, determination of calcitonin and cAMP is discussed in detail. This is a practically and clinically oriented monograph for working in the fields of internal medicine, endocrinology, and laboratory medicine.

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Calcium metabolism is regulated by three specific hormones: parathor- mone, calciferol metabolites, and calcitonin. Cyclic AMP also plays an important part in calcium regulation, and its concentration in urine can be taken as a measure of parathyroid function. Methods for quantitative measurement of, e.g. calcium-regulating hormones and vitamin D metabolites as well as of cAMP are absolutely necessary for safe differential diagnosis of diseases of the parathyroid like hypo- and hyperparathyroidism. In this monograph all presently available methods are summarized as to whether they can already be obtained as test kits or are still being tested. Radioimmunoassays for the various peptide fragments such as 53-84, 44-68, 28-48, and 1-34 are described, as are immunochemiluminescence methods and adenylate cyclase bioassays. The same is true for the vitamin D metabolites, where the various assays for calcidiol and calcitriol such as HPLC, protein-binding assay with second antibody separation, and double antibody RIA are de§ scribed. Finally, determination of calcitonin and cAMP is discussed in detail. This is a practically and clinically oriented monograph for working in the fields of internal medicine, endocrinology, and laboratory medicine.
Content:
Front Matter....Pages I-XI
Front Matter....Pages 1-1
Clinical Application of Intact Parathyroid Hormone Determination....Pages 3-23
Clinical Use of Vitamin D Metabolite Assays (Calcidiol and Calcitriol)....Pages 24-47
Diagnostic Value of the Peptides from the Calcitonin Gene....Pages 48-59
An Immunoassay Vial for Improved Assay Performance....Pages 60-66
Front Matter....Pages 67-67
Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53–84) with Tyrosylated Peptide as Tracer....Pages 69-83
Sensitive Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53–84) to Diagnose Hypoparathyroid Conditions: Guidelines for the Establishment of Highly Sensitive Assays....Pages 84-95
Rapid Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53–84) as a Diagnostic Aid in Hypercalcemic Crisis....Pages 96-106
Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 44–68) with Tyrosylated Peptide as Tracer....Pages 107-115
Purification of 125Iodine-Labeled Tyrosylated Human Parathyroid Hormone Fragment (Residues 44–68), Optimized by Reverse-Phase High-Performance Liquid Chromatography....Pages 116-126
Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 28–48) with Tyrosylated Peptide as Tracer....Pages 127-136
Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 1–34) with Biotinylated Peptide as Tracer....Pages 137-150
Measurement of Intact Parathyroid Hormone by an Extracting Two-Site Immunoradiometric Assay....Pages 151-166
Measurement of Intact Parathyroid Hormone by a Two-Site Immunochemiluminometric Assay....Pages 167-189
Sensitive Adenylate Cyclase Bioassay for Parathyroid Hormone....Pages 190-202
Immunological Properties of Asparagine versus Aspartic Acid at Residue 76 of Human Parathyroid Hormone (Residues 53–84)....Pages 203-209
Front Matter....Pages 211-211
Parathyroid Hormone-Related Protein....Pages 213-227
Front Matter....Pages 229-229
Plasma and Urinary Cyclic AMP, Assay and Clinical Application....Pages 231-243
Front Matter....Pages 245-245
Simultaneous Determination of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 by High-Performance Liquid Chromatography....Pages 247-257
Competitive Protein-Binding Assay for the Diagnosis of Hyper- and Hypovitaminosis D....Pages 258-279
A Competitive Protein-Binding Assay with Second Antibody Separation for the Diagnosis of Hyper- and Hypovitaminosis D....Pages 280-285
Front Matter....Pages 245-245
Interlaboratory Comparison of Calcidiol Determination....Pages 286-299
A Sensitive Radioimmunoassay for 1,25-Dihydroxyvitamin D3 (Calcitriol) after High-Performance Liquid Chromatography of Plasma or Serum Extracts....Pages 300-317
A Double Antibody Radioimmunoassay for Measurement of 1?,25-Dihydroxyvitamin D in Serum....Pages 318-333
Competitive Protein-Binding Assay of Calcitriol with an Advanced Preparation of Bovine Calf Thymus Cytosol....Pages 334-345
Front Matter....Pages 347-347
Design of New Methods for Measuring Calcitonin and Related Peptides Using Monoclonal Antipeptide Antibodies....Pages 349-364
Back Matter....Pages 365-370

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Calcium metabolism is regulated by three specific hormones: parathor- mone, calciferol metabolites, and calcitonin. Cyclic AMP also plays an important part in calcium regulation, and its concentration in urine can be taken as a measure of parathyroid function. Methods for quantitative measurement of, e.g. calcium-regulating hormones and vitamin D metabolites as well as of cAMP are absolutely necessary for safe differential diagnosis of diseases of the parathyroid like hypo- and hyperparathyroidism. In this monograph all presently available methods are summarized as to whether they can already be obtained as test kits or are still being tested. Radioimmunoassays for the various peptide fragments such as 53-84, 44-68, 28-48, and 1-34 are described, as are immunochemiluminescence methods and adenylate cyclase bioassays. The same is true for the vitamin D metabolites, where the various assays for calcidiol and calcitriol such as HPLC, protein-binding assay with second antibody separation, and double antibody RIA are de§ scribed. Finally, determination of calcitonin and cAMP is discussed in detail. This is a practically and clinically oriented monograph for working in the fields of internal medicine, endocrinology, and laboratory medicine.
Content:
Front Matter....Pages I-XI
Front Matter....Pages 1-1
Clinical Application of Intact Parathyroid Hormone Determination....Pages 3-23
Clinical Use of Vitamin D Metabolite Assays (Calcidiol and Calcitriol)....Pages 24-47
Diagnostic Value of the Peptides from the Calcitonin Gene....Pages 48-59
An Immunoassay Vial for Improved Assay Performance....Pages 60-66
Front Matter....Pages 67-67
Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53–84) with Tyrosylated Peptide as Tracer....Pages 69-83
Sensitive Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53–84) to Diagnose Hypoparathyroid Conditions: Guidelines for the Establishment of Highly Sensitive Assays....Pages 84-95
Rapid Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53–84) as a Diagnostic Aid in Hypercalcemic Crisis....Pages 96-106
Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 44–68) with Tyrosylated Peptide as Tracer....Pages 107-115
Purification of 125Iodine-Labeled Tyrosylated Human Parathyroid Hormone Fragment (Residues 44–68), Optimized by Reverse-Phase High-Performance Liquid Chromatography....Pages 116-126
Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 28–48) with Tyrosylated Peptide as Tracer....Pages 127-136
Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 1–34) with Biotinylated Peptide as Tracer....Pages 137-150
Measurement of Intact Parathyroid Hormone by an Extracting Two-Site Immunoradiometric Assay....Pages 151-166
Measurement of Intact Parathyroid Hormone by a Two-Site Immunochemiluminometric Assay....Pages 167-189
Sensitive Adenylate Cyclase Bioassay for Parathyroid Hormone....Pages 190-202
Immunological Properties of Asparagine versus Aspartic Acid at Residue 76 of Human Parathyroid Hormone (Residues 53–84)....Pages 203-209
Front Matter....Pages 211-211
Parathyroid Hormone-Related Protein....Pages 213-227
Front Matter....Pages 229-229
Plasma and Urinary Cyclic AMP, Assay and Clinical Application....Pages 231-243
Front Matter....Pages 245-245
Simultaneous Determination of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 by High-Performance Liquid Chromatography....Pages 247-257
Competitive Protein-Binding Assay for the Diagnosis of Hyper- and Hypovitaminosis D....Pages 258-279
A Competitive Protein-Binding Assay with Second Antibody Separation for the Diagnosis of Hyper- and Hypovitaminosis D....Pages 280-285
Front Matter....Pages 245-245
Interlaboratory Comparison of Calcidiol Determination....Pages 286-299
A Sensitive Radioimmunoassay for 1,25-Dihydroxyvitamin D3 (Calcitriol) after High-Performance Liquid Chromatography of Plasma or Serum Extracts....Pages 300-317
A Double Antibody Radioimmunoassay for Measurement of 1?,25-Dihydroxyvitamin D in Serum....Pages 318-333
Competitive Protein-Binding Assay of Calcitriol with an Advanced Preparation of Bovine Calf Thymus Cytosol....Pages 334-345
Front Matter....Pages 347-347
Design of New Methods for Measuring Calcitonin and Related Peptides Using Monoclonal Antipeptide Antibodies....Pages 349-364
Back Matter....Pages 365-370
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