Ebook: Molecular Biology of Erythropoiesis

This volume presents the proceedings of the Fourth Annual Symposium on the Molecular Biology of Hemopoiesis, held in Reno, Nevada, November 1 and 2, 1988. Its focus on erythropoiesis represents an attempt to cover a rapidly expanding field, which has gone from elegant studies of erythro­ poietin physiology, to molecular biology, to clinical applications and again to physiology. The rapid development has been made possible by cloning of erythropoietin gene and the availability of recombinant hormone. The regulation of heme and its derivatives has also been aided by techniques of molecular biology; there is now a concerted effort to better understand how these enzymes contribute to proliferation, differentiation and maturation of the erythron. Globin gene derrangements have been targets of recent research in an attempt to correct the defect by genetic engineering. In the chapters of this book, several groups "expressed" their views on this subject. Finally, we analyze various regulators of erythropoiesis, both in vivo and in vitro. Dr. Richard Levere was a pioneer in many studies of heme metabolism and of erythropoiesis. He has been a generous supporter of research in this field and of our past meetings. It is only. fitting that this volume should be dedicated to him.

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Content:
Front Matter....Pages i-xiii
Erythropoietin: From Mountain Top to Bedside....Pages 1-7
Serum Erythropoietin and Hemoglobin Affinity for Oxygen in Patients Phlebotomized for Polycythemia Vera....Pages 9-15
Erythropoietin and the Polycythemia of High-Altitude Dwellers....Pages 17-21
Enhanced Effect Of Increased Erythrocyte Production Rate On Plasma Erythropoietin Levels Of Mice During Subsequent Exposure To Hypobaria....Pages 23-27
Metabolism of Recombinant Human Erythropoietin in the Rat....Pages 29-38
Regulation of Extrarenal Erythropoietin Production....Pages 39-51
Structure and Role of Carbohydrate in Human Erythropoietin....Pages 53-67
Cellular Localization of Erythropoietin Gene Transcription....Pages 69-74
Regulation of the Erythropoietin Gene....Pages 75-85
Studies of the Effect of Erythropoietin on Heme Synthesis....Pages 87-94
Effects of Hemin on Erythropoiesis....Pages 95-102
The Effect of Dimethyl Sulfoxide on Heme Synthesis and the Acute Phase Reaction in Human HepG2 Hepatoma Cells....Pages 103-114
Cytochrome P450 Dependent Arachidonic Acid Metabolism in Hemopoietic Cells....Pages 115-121
Regulation of Heme Biosynthesis in Chick Embryo Liver Cells....Pages 123-133
Long-Term Expression of the Human ?-Globin Gene After Retroviral Transfer into Pluripotent Hematopoietic Stem Cells of the Mouse....Pages 135-148
Retroviral Gene Transfer in Mice: The Use of a Unique Packaging Line Improves Efficiency....Pages 149-160
Expression of the Globin Genes and Hematopoiesis in Beta-Thalassemic Mice....Pages 161-176
Butyric Acid Modulates Developmental Globin Gene Switching in Man and Sheep....Pages 177-183
Tumor Necrosis Factor ? and the Anemia of Chronic Disease: Effects of Chronic Exposure to TNF on Erythropoiesis in Vivo....Pages 185-196
Erythropoiesis in Cancer Patients Undergoing Immunotherapy....Pages 197-204
Erythropoiesis in Murine Long Term Marrow Cultures....Pages 205-209
Expression of Homeobox Genes in Human Erythroleukemia Cells....Pages 211-219
Back Matter....Pages 221-227

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Content:
Front Matter....Pages i-xiii
Erythropoietin: From Mountain Top to Bedside....Pages 1-7
Serum Erythropoietin and Hemoglobin Affinity for Oxygen in Patients Phlebotomized for Polycythemia Vera....Pages 9-15
Erythropoietin and the Polycythemia of High-Altitude Dwellers....Pages 17-21
Enhanced Effect Of Increased Erythrocyte Production Rate On Plasma Erythropoietin Levels Of Mice During Subsequent Exposure To Hypobaria....Pages 23-27
Metabolism of Recombinant Human Erythropoietin in the Rat....Pages 29-38
Regulation of Extrarenal Erythropoietin Production....Pages 39-51
Structure and Role of Carbohydrate in Human Erythropoietin....Pages 53-67
Cellular Localization of Erythropoietin Gene Transcription....Pages 69-74
Regulation of the Erythropoietin Gene....Pages 75-85
Studies of the Effect of Erythropoietin on Heme Synthesis....Pages 87-94
Effects of Hemin on Erythropoiesis....Pages 95-102
The Effect of Dimethyl Sulfoxide on Heme Synthesis and the Acute Phase Reaction in Human HepG2 Hepatoma Cells....Pages 103-114
Cytochrome P450 Dependent Arachidonic Acid Metabolism in Hemopoietic Cells....Pages 115-121
Regulation of Heme Biosynthesis in Chick Embryo Liver Cells....Pages 123-133
Long-Term Expression of the Human ?-Globin Gene After Retroviral Transfer into Pluripotent Hematopoietic Stem Cells of the Mouse....Pages 135-148
Retroviral Gene Transfer in Mice: The Use of a Unique Packaging Line Improves Efficiency....Pages 149-160
Expression of the Globin Genes and Hematopoiesis in Beta-Thalassemic Mice....Pages 161-176
Butyric Acid Modulates Developmental Globin Gene Switching in Man and Sheep....Pages 177-183
Tumor Necrosis Factor ? and the Anemia of Chronic Disease: Effects of Chronic Exposure to TNF on Erythropoiesis in Vivo....Pages 185-196
Erythropoiesis in Cancer Patients Undergoing Immunotherapy....Pages 197-204
Erythropoiesis in Murine Long Term Marrow Cultures....Pages 205-209
Expression of Homeobox Genes in Human Erythroleukemia Cells....Pages 211-219
Back Matter....Pages 221-227
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