Ebook: Epstein-Barr Virus and Human Disease • 1988

Epstein-Barr virus (EBV) is one of the most widespread human viruses, with over 80% of the general population exposed by young adulthood, as determined by antibody studies. Initial infection usu­ ally occurs during childhood or the teenage years. It is clear that, de­ pending on the age of the recipient, clinical manifestations of the primary infection can vary. It has been known for 20 years that EBV is the etiologic agent of acute infectious mononucleosis (IM) and is also closely associated with African Burkitt's lymphoma (BL) and naso­ it is a pharyngeal carcinoma (NPC). There is increasing evidence that factor in the etiology of B-celllymphomas, which arise at a high fre­ quency in immunodeficient populations. EBV may also contribute to the development of autoimmune disease. Thus, this virus continues to attract world-wide attention. The major target cell for EBV infection has now been conclusively identified as the complement receptor-type 2 (CR2), the Cd/Cdg 3 3 receptor on B lymphocyte. It is apparent, however, that other cells also can become infected by EBV, such as epithelial cells in the parotid gland and other epithelial cells in the upper respiratory tract. This might help account for the EBV-assodated carcinomas of the upper respiratory tract. The first in a series of international symposia on EBV -associated diseases focused primarily on Burkitt's-type lymphomas or on NPC (Kyoto 1977; Dusseldorf 1980; Kuala Lumpur 1982) and emphasized mainly the clinical elements of these diseases. Subsequent symposia (Loutraki, 1984; St.

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Content:
Front Matter....Pages i-xxvii
Front Matter....Pages 1-1
Epstein-Barr Virus Transformation of B Lymphocytes: Molecular Pathogenesis....Pages 3-15
Role of The Zebra Protein in the Switch Between Epstein-Barr Virus Latency and Replication....Pages 17-35
DNA:EBMA-1 Interactions and Latency of Epstein-Barr Virus....Pages 37-42
The EBV DR Enhancer Contains two Functionally Different Domains: A and B.....Pages 43-47
Identification of the Gene Product Encoded by the Notl Repeats of the EBV Genome....Pages 49-53
Effect of the BZLF1 Transactivator on Different Classes of EBV Promoters....Pages 55-60
EBNA Expression and CpG Methylation of Viral DNA in EBV Associated Tumours....Pages 61-62
Transcription and Protein Expression Pattern of EBV in Freshly Infected Lymphoid Cells....Pages 63-67
Characterization of a cDNA of Epstein-Barr Virus DNase Gene....Pages 69-74
The 5’Flanking Region of the Gene for EBNA2 Contains a Cell Type Specific cis-Acting Regulatory Element that Activates Transcription in Transfected B-Cells....Pages 75-78
Establishment and Characterization of a Tumor Cell Line from Nasopharyngeal Carcinoma Tissue....Pages 79-82
Front Matter....Pages 83-83
EBV DNA Structure and Expression in EBV-Induced Proliferations....Pages 85-97
The Role of Differentially Expressed EB-Viral and Cellular Genes for Development of Virus-Related Tumors and Possibilities of the Control of These Diseases....Pages 99-103
Detection of Proteins Binding to the EBV EBNA-2 Promoter....Pages 105-112
Anti EBNA-1 Protein Induced with TPA....Pages 113-118
Characterization of the EBV Alkaline Exonuclease Expressed in a Recombinant Prokaryotic System....Pages 119-124
Immunocytochemistry of EBV Envelope Glycoproteins on Freeze-Fractured Nuclear Membranes of Virus Producer Cells....Pages 125-130
Detection of a Transformation-Incompetent Epstein-Barr Virus Genotype in a Seronegative Host....Pages 131-135
Propagation and Cytogenetic Analysis of NPC Biopsies in Nude Mice: Evidence for a Translocation in Chromosome #8 at 8q 2 4 which is the Localization of the c-myc Gene....Pages 137-140
Epstein-Barr Virus Gene Expression in Lymphomas Induced by the Virus in the Cottontop Tamarin....Pages 141-144
Front Matter....Pages 83-83
The DNA Demethylating Agent 5-Azacytidine Induces the Expression of EBNA 2–6 and LMP in Burkitt Lymphoma Lines....Pages 145-148
Epstein-Barr Virus mRNA Expression in Biopsies of Lymphoma Induced by the Virus in the Cottontop Tamarin....Pages 149-153
Establishment of Salivary Gland Epithelial Cell Lines from Patients with Sjogren’s Syndrome and Normal Individual....Pages 155-161
The Transforming Activity of a Nasopharyngeal Carcinoma-Derived Epstein-Barr Virus Isolate....Pages 163-167
Front Matter....Pages 169-169
Immune Control Mechanisms over EBV Infection....Pages 171-178
Epstein-Barr Virus Infection and Immunoregulation....Pages 179-187
Helper T Lymphocyte Proliferation is Stimulated by the Presence of EBV-Carrying B Lymphocytes....Pages 189-193
Role of the Recognition of Virion Antigens in the T-Cell Mediated Inhibition of EBV-Induced B-Cell Transformation....Pages 195-200
An Analysis of the Cytotoxic T-Cell Response of A- and B-Type EB Virus....Pages 201-206
The Immune Response to Glycine-Rich Sequences of Epstein-Barr Nuclear Antigen-1 (EBNA-1) in Autoimmunity: B and T Cell Epitopes....Pages 207-212
Cytotoxic Tumor Infiltrating Lymphocytes in Nasopharyngeal Carcinoma....Pages 213-218
Generation of Cytotoxic T Cells Against EBV Infected B Lymphocytes....Pages 219-222
T Cell Response to Peptides Encompassing a 5 Amino Acid Sequence Shared by the HLA DW4 DR Beta 1 Chain and the Epstein Barr Virus Glycoprotein GP110....Pages 223-226
Growth Stimulation and Immunoglobulin (Ig) Secretion in Epstein-Barr Virus (Ebv)-Infected B Cells by Interleukin 6 (IL-6)....Pages 227-229
Presence of EBV Infected Cells in the Bone Marrow from Transplant Donors....Pages 231-235
Front Matter....Pages 237-237
Clinical Syndromes Associated with Epstein-Barr Virus....Pages 239-250
The Natural History and Treatment of EBV Infections and Their Nonmalignant Complications....Pages 251-260
Epstein-Barr Virus Polymorphic Diffuse Lymphoma Associated with Leukemia and with Congenital Immunodeficiencies....Pages 261-266
EB-Viral Expression Depends on the Differentiated Status of Epithelial Cells in Oral Hairy L eukoplakia....Pages 267-270
EBV-Associated Lymphoproliferative Disorders in a Large Transplant Population....Pages 271-275
Front Matter....Pages 237-237
EBV Associated Head and Neck Tumours and Thymic Lesions....Pages 277-280
Expression of EBV in the Nasopharyngeal Carcinoma-Derived Nude Mouse Tumour, C15....Pages 281-286
Killer Cell Defect and Lack or Loss of Antibodies to Epstein-Barr Virus Glycoproteins in Chronic Active EBV....Pages 287-291
Characterization of Cell Types and EBV Gene Expression in Lymphoproliferative Diseases (LPD) of Patients Receiving Bone Marrow Transplants (BMT) after T-Cell Depletion....Pages 293-296
Epstein-Barr (EBV) Viral DNA in Tissues of Immunocompetent and Immunodeficient Children....Pages 297-301
Different Cell Susceptibility to Epstein-Barr Virus Infection in a Patient with X-Linked Lymphoproliferative Syndrome....Pages 303-306
Front Matter....Pages 307-307
Detection of EBV Specific IgA Antibodies to EA, MA and EBNA-1 Recombinant Proteins in NPC Patients and Controls....Pages 309-313
Challenges in the Treatment of EBV-Associated Diseases: New Approaches....Pages 315-327
Epstein-Barr Virus Replication and Gene Expression in Oral “Hairy” Leukoplakia....Pages 329-331
Changes in virus-host genome relationship in two sublines of the same Burkitt’s lymphoma....Pages 333-339
Molecular Weight Variation of EBNAs as a Means to Identify Different Epstein-Barr Virus Isolates (EBNotyping)....Pages 341-347
Cell-Mediated Responses to Epstein-Barr Virus Lytic Antigens....Pages 349-353
Successful Therapy for EBV Associated B Cell Lymphoproliferative Disorders in Immunodeficiency Using Alpha Interferon and Intravenous Immunoglobulin....Pages 355-360
Serological Correlation of HHV-6 and EBV Infections in Infectious Mononucleosis....Pages 361-367
Natural Epstein-Barr Virus Isolates from Southern China and California Differ in the Bam Hl I Region....Pages 369-374
Antibodies Against EBNA-1 Derived Synthetic Peptide in Various Patient Groups....Pages 375-378
Nasopharyngeal Carcinoma (NPC): The Influence of Different Types of Interferon (IFN) on Peripheral Blood Lymphocytes....Pages 379-383
Early Diagnosis of Nasopharyngeal Carcinoma: A Multi-Pronged Approach....Pages 385-389
Front Matter....Pages 391-391
Cellular Receptors for Epstein-Barr Virus....Pages 393-403
The Post-Infectious Chronic Fatigue Syndrome....Pages 405-438
Front Matter....Pages 391-391
Post Viral Chronic Fatigue Syndrome: Persistence of Epstein-Barr Virus DNA in Muscle....Pages 439-444
The Post-Infectious Chronic Fatigue Syndrome: Cell Surface Expression of LFA-1 And ICAM-1....Pages 445-447
Front Matter....Pages 449-449
Epstein-Barr Virus (EBV)-Induced Transformation and EBV Genome Reorganization in Lymphoblastoid Cell Lines Established from Patients with AIDS Following Enhancement with Chemical Carcinogens....Pages 451-460
Human Herpesvirus-6 (Human B-Lymphotrophic Virus)....Pages 461-472
Interactions of Epstein-Barr Virus with Human Retroviruses....Pages 473-480
An Environmental Plant Factor Enhancing EBV-Specific Events in East Africa: Reduction of Killer T-Cell Function and Its Protection by Hot Spring Water....Pages 481-484
Epstein-Barr Virus Antigens and Genome in Lymph Nodes from Patients with Persistent Generalized Lymphadenopathy (PGL)....Pages 485-488
Interaction of HBLV (HHV-6) With EBV and HIV....Pages 489-494
Enhanced SV40 Immortalization of Primary Human Epidermal Cells Following Phorbol Ester Dependent EBV Transformation....Pages 495-500
Food Extracts from High Risk Areas for NPC Induce an EBV Early Promoter....Pages 501-504
Back Matter....Pages 505-522

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Content:
Front Matter....Pages i-xxvii
Front Matter....Pages 1-1
Epstein-Barr Virus Transformation of B Lymphocytes: Molecular Pathogenesis....Pages 3-15
Role of The Zebra Protein in the Switch Between Epstein-Barr Virus Latency and Replication....Pages 17-35
DNA:EBMA-1 Interactions and Latency of Epstein-Barr Virus....Pages 37-42
The EBV DR Enhancer Contains two Functionally Different Domains: A and B.....Pages 43-47
Identification of the Gene Product Encoded by the Notl Repeats of the EBV Genome....Pages 49-53
Effect of the BZLF1 Transactivator on Different Classes of EBV Promoters....Pages 55-60
EBNA Expression and CpG Methylation of Viral DNA in EBV Associated Tumours....Pages 61-62
Transcription and Protein Expression Pattern of EBV in Freshly Infected Lymphoid Cells....Pages 63-67
Characterization of a cDNA of Epstein-Barr Virus DNase Gene....Pages 69-74
The 5’Flanking Region of the Gene for EBNA2 Contains a Cell Type Specific cis-Acting Regulatory Element that Activates Transcription in Transfected B-Cells....Pages 75-78
Establishment and Characterization of a Tumor Cell Line from Nasopharyngeal Carcinoma Tissue....Pages 79-82
Front Matter....Pages 83-83
EBV DNA Structure and Expression in EBV-Induced Proliferations....Pages 85-97
The Role of Differentially Expressed EB-Viral and Cellular Genes for Development of Virus-Related Tumors and Possibilities of the Control of These Diseases....Pages 99-103
Detection of Proteins Binding to the EBV EBNA-2 Promoter....Pages 105-112
Anti EBNA-1 Protein Induced with TPA....Pages 113-118
Characterization of the EBV Alkaline Exonuclease Expressed in a Recombinant Prokaryotic System....Pages 119-124
Immunocytochemistry of EBV Envelope Glycoproteins on Freeze-Fractured Nuclear Membranes of Virus Producer Cells....Pages 125-130
Detection of a Transformation-Incompetent Epstein-Barr Virus Genotype in a Seronegative Host....Pages 131-135
Propagation and Cytogenetic Analysis of NPC Biopsies in Nude Mice: Evidence for a Translocation in Chromosome #8 at 8q 2 4 which is the Localization of the c-myc Gene....Pages 137-140
Epstein-Barr Virus Gene Expression in Lymphomas Induced by the Virus in the Cottontop Tamarin....Pages 141-144
Front Matter....Pages 83-83
The DNA Demethylating Agent 5-Azacytidine Induces the Expression of EBNA 2–6 and LMP in Burkitt Lymphoma Lines....Pages 145-148
Epstein-Barr Virus mRNA Expression in Biopsies of Lymphoma Induced by the Virus in the Cottontop Tamarin....Pages 149-153
Establishment of Salivary Gland Epithelial Cell Lines from Patients with Sjogren’s Syndrome and Normal Individual....Pages 155-161
The Transforming Activity of a Nasopharyngeal Carcinoma-Derived Epstein-Barr Virus Isolate....Pages 163-167
Front Matter....Pages 169-169
Immune Control Mechanisms over EBV Infection....Pages 171-178
Epstein-Barr Virus Infection and Immunoregulation....Pages 179-187
Helper T Lymphocyte Proliferation is Stimulated by the Presence of EBV-Carrying B Lymphocytes....Pages 189-193
Role of the Recognition of Virion Antigens in the T-Cell Mediated Inhibition of EBV-Induced B-Cell Transformation....Pages 195-200
An Analysis of the Cytotoxic T-Cell Response of A- and B-Type EB Virus....Pages 201-206
The Immune Response to Glycine-Rich Sequences of Epstein-Barr Nuclear Antigen-1 (EBNA-1) in Autoimmunity: B and T Cell Epitopes....Pages 207-212
Cytotoxic Tumor Infiltrating Lymphocytes in Nasopharyngeal Carcinoma....Pages 213-218
Generation of Cytotoxic T Cells Against EBV Infected B Lymphocytes....Pages 219-222
T Cell Response to Peptides Encompassing a 5 Amino Acid Sequence Shared by the HLA DW4 DR Beta 1 Chain and the Epstein Barr Virus Glycoprotein GP110....Pages 223-226
Growth Stimulation and Immunoglobulin (Ig) Secretion in Epstein-Barr Virus (Ebv)-Infected B Cells by Interleukin 6 (IL-6)....Pages 227-229
Presence of EBV Infected Cells in the Bone Marrow from Transplant Donors....Pages 231-235
Front Matter....Pages 237-237
Clinical Syndromes Associated with Epstein-Barr Virus....Pages 239-250
The Natural History and Treatment of EBV Infections and Their Nonmalignant Complications....Pages 251-260
Epstein-Barr Virus Polymorphic Diffuse Lymphoma Associated with Leukemia and with Congenital Immunodeficiencies....Pages 261-266
EB-Viral Expression Depends on the Differentiated Status of Epithelial Cells in Oral Hairy L eukoplakia....Pages 267-270
EBV-Associated Lymphoproliferative Disorders in a Large Transplant Population....Pages 271-275
Front Matter....Pages 237-237
EBV Associated Head and Neck Tumours and Thymic Lesions....Pages 277-280
Expression of EBV in the Nasopharyngeal Carcinoma-Derived Nude Mouse Tumour, C15....Pages 281-286
Killer Cell Defect and Lack or Loss of Antibodies to Epstein-Barr Virus Glycoproteins in Chronic Active EBV....Pages 287-291
Characterization of Cell Types and EBV Gene Expression in Lymphoproliferative Diseases (LPD) of Patients Receiving Bone Marrow Transplants (BMT) after T-Cell Depletion....Pages 293-296
Epstein-Barr (EBV) Viral DNA in Tissues of Immunocompetent and Immunodeficient Children....Pages 297-301
Different Cell Susceptibility to Epstein-Barr Virus Infection in a Patient with X-Linked Lymphoproliferative Syndrome....Pages 303-306
Front Matter....Pages 307-307
Detection of EBV Specific IgA Antibodies to EA, MA and EBNA-1 Recombinant Proteins in NPC Patients and Controls....Pages 309-313
Challenges in the Treatment of EBV-Associated Diseases: New Approaches....Pages 315-327
Epstein-Barr Virus Replication and Gene Expression in Oral “Hairy” Leukoplakia....Pages 329-331
Changes in virus-host genome relationship in two sublines of the same Burkitt’s lymphoma....Pages 333-339
Molecular Weight Variation of EBNAs as a Means to Identify Different Epstein-Barr Virus Isolates (EBNotyping)....Pages 341-347
Cell-Mediated Responses to Epstein-Barr Virus Lytic Antigens....Pages 349-353
Successful Therapy for EBV Associated B Cell Lymphoproliferative Disorders in Immunodeficiency Using Alpha Interferon and Intravenous Immunoglobulin....Pages 355-360
Serological Correlation of HHV-6 and EBV Infections in Infectious Mononucleosis....Pages 361-367
Natural Epstein-Barr Virus Isolates from Southern China and California Differ in the Bam Hl I Region....Pages 369-374
Antibodies Against EBNA-1 Derived Synthetic Peptide in Various Patient Groups....Pages 375-378
Nasopharyngeal Carcinoma (NPC): The Influence of Different Types of Interferon (IFN) on Peripheral Blood Lymphocytes....Pages 379-383
Early Diagnosis of Nasopharyngeal Carcinoma: A Multi-Pronged Approach....Pages 385-389
Front Matter....Pages 391-391
Cellular Receptors for Epstein-Barr Virus....Pages 393-403
The Post-Infectious Chronic Fatigue Syndrome....Pages 405-438
Front Matter....Pages 391-391
Post Viral Chronic Fatigue Syndrome: Persistence of Epstein-Barr Virus DNA in Muscle....Pages 439-444
The Post-Infectious Chronic Fatigue Syndrome: Cell Surface Expression of LFA-1 And ICAM-1....Pages 445-447
Front Matter....Pages 449-449
Epstein-Barr Virus (EBV)-Induced Transformation and EBV Genome Reorganization in Lymphoblastoid Cell Lines Established from Patients with AIDS Following Enhancement with Chemical Carcinogens....Pages 451-460
Human Herpesvirus-6 (Human B-Lymphotrophic Virus)....Pages 461-472
Interactions of Epstein-Barr Virus with Human Retroviruses....Pages 473-480
An Environmental Plant Factor Enhancing EBV-Specific Events in East Africa: Reduction of Killer T-Cell Function and Its Protection by Hot Spring Water....Pages 481-484
Epstein-Barr Virus Antigens and Genome in Lymph Nodes from Patients with Persistent Generalized Lymphadenopathy (PGL)....Pages 485-488
Interaction of HBLV (HHV-6) With EBV and HIV....Pages 489-494
Enhanced SV40 Immortalization of Primary Human Epidermal Cells Following Phorbol Ester Dependent EBV Transformation....Pages 495-500
Food Extracts from High Risk Areas for NPC Induce an EBV Early Promoter....Pages 501-504
Back Matter....Pages 505-522
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