Ebook: Rapid Methods in Clinical Microbiology: Present Status and Future Trends

The papers published herein comprise the presentations given at the eighteenth of an annual series of clinical symposia arranged under the auspices of the Eastern Pennsylvania Branch of the American Society for Microbiology. This symposium allowed approximately 200 persons to gather and exchange ideas on the rapid laboratory diagnosis of infectious diseases. The institution of the Diagnosis Related Group (DRG) method for reimbursement by both government agencies and private insurance carriers has provided a financial aspect to the established clinical reasons for rapid laboratory diagnosis. Now the health of the institution, as well as the patient, is dependent on a timely diagnosis and, hopefully, cure. Accordingly, the goal of this symposium was to present the latest developments in "same-day microbiology". In the face of stable or diminishing resources, the laboratory director is presented with many choices. Do nucleic acid probes, non­ instrumental ELISA techniques, or time-resolved fluorometry have a place in his or her laboratory? Should the laboratory test for newly described human pathogens such as human immunodeficiency virus or human papilloma virus? Can rapid techniques supplant conventional methods? Or are they merely adjunctive? This symposium attempted to assist in the formulation of informed decisions. Bruce Kleger Donald Jungkind Eileen Rinks Linda A. Miller vii ACKNOWLEDGEMENTS We would like to thank the Eastern Pennsylvania Branch of t~e American Society for Microbiology for sponsoring this symposium and for making this publication possible. We especially thank the Symposium Committee for their diligent work in organizing an informative and successful symposium.

---

---

Content:
Front Matter....Pages i-xii
“Where we’ve been and where we may be going”....Pages 1-9
The Use of DNA Probes to Detect and Identify Microorganisms....Pages 11-35
The Influence of Rapid Diagnosis of Streptococcal Infection on Pharyngitis and Rheumatic Fever....Pages 37-50
The Use of DNA Probes for Rapidly Identifying Cultures of Mycobacterium ....Pages 51-56
Use of DNA Probes for the Diagnosis of Infections Caused by Mycoplasma Pneumoniae and Legionellae- A Review....Pages 57-69
Multicenter Clinical Evaluation of the Du Pont HERPCHEKTM HSV Elisa, a New Rapid Diagnostic Test for the Direct Detection of Herpes Simplex Virus....Pages 71-76
Non-Culture Tests for the Diagnosis of Gonorrhea....Pages 77-88
Nucleic Acid Hybridization as a Diagnostic Tool for the Detection of Human Papillomaviruses....Pages 89-109
The Rapid Detection of Clinically Significant Bacteria....Pages 111-114
Rapid Diagnosis of Viral Infections....Pages 115-121
Time-Resolved Fluorometry: Principles and Application to Clinical Microbiology and DNA Probe Technology....Pages 123-130
The Clinical Impact of Automated Susceptibility Reporting Using a Computer Interface....Pages 131-142
Back Matter....Pages 143-146

---

Content:
Front Matter....Pages i-xii
“Where we’ve been and where we may be going”....Pages 1-9
The Use of DNA Probes to Detect and Identify Microorganisms....Pages 11-35
The Influence of Rapid Diagnosis of Streptococcal Infection on Pharyngitis and Rheumatic Fever....Pages 37-50
The Use of DNA Probes for Rapidly Identifying Cultures of Mycobacterium ....Pages 51-56
Use of DNA Probes for the Diagnosis of Infections Caused by Mycoplasma Pneumoniae and Legionellae- A Review....Pages 57-69
Multicenter Clinical Evaluation of the Du Pont HERPCHEKTM HSV Elisa, a New Rapid Diagnostic Test for the Direct Detection of Herpes Simplex Virus....Pages 71-76
Non-Culture Tests for the Diagnosis of Gonorrhea....Pages 77-88
Nucleic Acid Hybridization as a Diagnostic Tool for the Detection of Human Papillomaviruses....Pages 89-109
The Rapid Detection of Clinically Significant Bacteria....Pages 111-114
Rapid Diagnosis of Viral Infections....Pages 115-121
Time-Resolved Fluorometry: Principles and Application to Clinical Microbiology and DNA Probe Technology....Pages 123-130
The Clinical Impact of Automated Susceptibility Reporting Using a Computer Interface....Pages 131-142
Back Matter....Pages 143-146
....