Ebook: Progress in Plant Protoplast Research: Proceedings of the 7th International Protoplast Symposium, Wageningen, the Netherlands, December 6–11, 1987

`The amount of information included in the volume enables the reader to rapidly obtain a general overview of this field of research. In this respect it is of value to scientists already established in the field of plant genetic manipulation and those wishing to enter this exciting area of plant research.'
M.R. Davey, Plant, Cell and Environment, Vol. 12, No. 5, 1989.

`By the wide volume of data presented as well as topicality of the problems and the high scientific content, the book is a valuable guide and source of information in a modern and important field of biological sciences, for phytologists, cytologists, geneticians and pecialists in biotechnology, in general.'
A. Brezeanu, Romanian Review of Biochemistry, 1989

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Content:
Front Matter....Pages I-XVII
Progress in Plant Protoplast Research....Pages 1-5
Obstacles and Perspectives in Protoplast Research....Pages 7-14
Aspects of Protoplast Culture and Plant Regeneration....Pages 15-25
Recent Developments in the Regeneration of Agronomically Important Crops from Protoplasts....Pages 27-31
Selection, Experimental Manipulation and Culture of Single Protoplasts....Pages 33-34
Uniparental, Fusant, and Chimeric Plants Regenerated from Protoplasts after Streak Plating in Agarose Gels....Pages 35-37
Electroporation-Mediated Enhancement of Plant Protoplast Division and Plant Regeneration....Pages 39-40
Selection of Potato Cell Suspension Protoplast Subpopulation by Rhodamine 123....Pages 41-42
Protoplast Culture Of Dioscorea Opposita Thunb. (D. Batatas DECNE)....Pages 43-44
Isolation and Culture of Buckwheat (Fagopyrum Esculentum Moench.) Callus Protoplasts....Pages 45-46
Partial Purification of a Cellulase Used for Protoplast Isolation from Barley (Hordeum vulgare)....Pages 47-48
Plant Regeneration from Cell Suspension Protoplasts of Festuca Arundinacea Schreb., Lolium Perenne L. and L. Multiflorum Lam....Pages 49-52
Isolation, Culture and Regeneration of Lolium Perenne and Lolium Multiflorum Protoplasts....Pages 53-54
Mature Plant Regeneration from Cultivated Protoplasts of Rice (Oryza Sativa L.)....Pages 55-56
The Plants Regenerated from Protoplasts of Suspension Cells of Rice(Oryza Sativa L)....Pages 57-58
Regeneration of Plantlets from Protoplasts of Poa Pratensis L. (Kentucky Bluegrass)....Pages 59-60
Somatic Embryogenesis and Plant Regeneration from Protoplast of Maize (Zea mays L.)....Pages 61-62
Protoplasts from Actinidia Deliciosa (Kiwi): Obtention and Culture....Pages 63-64
Colony Formation from Cell Suspension Culture Protoplasts of Kiwifruit....Pages 65-66
Isolation and Culture of Grapevine Protoplasts (Vitis Vinifera L.)....Pages 67-68
Studies Towards a Protoplast Isolation and Culture Procedure in Sugarbeet (Beta Vulgaris L.) Applicable to a Variety of Genotypes....Pages 69-70
Improvement of Protoplast Culture in Helianthus annuus (L)....Pages 71-72
Factors Influencing Protoplasts Plating Efficiency and Direct Somatic Embryogenesis Induction in Sunflower (Helianthus Annuus L.)....Pages 73-74
Comparison of Sunflower Protoplast Development in Liquid or Solidified Media....Pages 75-76
Plant Regeneration from Protoplasts of Rudbeckia and Dimorphotheca Species (Compositae)....Pages 77-78
Optimization of lettuce calli regeneration from protoplasts....Pages 79-80
Optimal Density and Osmolality of Protoplasts for Improved Cultural Performance....Pages 81-82
Development of Protoplast Culture Procedures in Brassica Crops Suitable for in Vitro Selection Herbicide Resistance Mutants and Cybrids....Pages 83-84
Somatic Embryogenesis in Stationary Phase Suspension Cultures Derived from Hypocotyl Protoplasts of Brassica Napus L.....Pages 85-90
Plant Regeneration from Cucumber Protoplasts....Pages 91-94
Callus Formation from Mesophyll Protoplasts of Forage Legume Astragalus Huangheensis Fu Et Lin....Pages 95-96
Preliminary Studies of Lupinus Protoplasts....Pages 97-98
Regeneration and Differentiation of Protoplasts in Grain Legumes....Pages 99-100
Plant Regeneration from Strawberry (Fragaria Ananassa) Mesophyll Protoplasts....Pages 101-102
Isolation and Culture of Apple Mesophyll Protoplasts....Pages 103-104
Rhizogenesis as an Intermediate Step towards Plant Regeneration of Protoplasts of Sour Cherry (Prunus Cerasus L.), Rosaceae....Pages 105-106
A Protoplast System for Populus Which will Support Genetic Engineering Research of Tree Species....Pages 107-108
Factors Influencing Digitalis Obscura Mesophyll Protoplast Development....Pages 109-110
Cotyledon Protoplasts from Solanum Melongena L. Regeneration to Fertile Plants....Pages 111-112
Isolation, Culture and Regeneration of Leaf Protoplasts in the Genus Capsicum....Pages 113-114
Isolation and Properties of Protoplasts of Theobroma Cacao L.....Pages 115-116
Plantlet Regeneration from Protoplasts of Levisticum Officinale Koch.and Its Cytological Investigation....Pages 117-118
Differences in developmental morphology of protoplasts from two cell lines of white spruce (Picea glauca)....Pages 119-120
Protoplast Development from Porphyra Linearis — An Edible Marine Red Alga....Pages 121-122
Structure and Physiology of the Protoplast Plasma Membrane....Pages 123-124
Why do Mesophyll Protoplasts Dedifferentiate?....Pages 125-131
Release of Phytotoxic Factors from Plant Cell Walls during Protoplast Isolation....Pages 133-134
Role of Peroxidase in Protoplast Development: The Activity and Molecular Forms of Peroxidase in Mitogenic and Non-Mitogenic Protoplasts....Pages 135-136
Measurement of Cytoplasmic Ca2+ and H+ in Barley Aleurone Protoplasts....Pages 137-138
The Effect of Secretion on Lipids and their Distribution in Barley Aleurone Protoplasts....Pages 139-142
Incidence of Growth Regulators on RNA/ DNA Levels in Protoplasts as Analysed by Acridine Orange Staining and Flow Cytometry....Pages 143-144
IAA, ABA Content and Metabolism in Maize Root Protoplasts....Pages 145-146
Some Metabolic Peculiarities of Isolated Protoplasts....Pages 147-148
Gene Transfer by Individual Manipulation of Plant Cells....Pages 149-150
Potentials of Protoplast Fusion in Plant Breeding Programmes....Pages 151-157
Use of Protoplasts on Plant Breeding: Cytoplasmic Aspects....Pages 159-168
Protoplast Fusion between Eruca Sativa and Brassica Napus — Analysis of Regenerated Plants....Pages 169-176
Fertility and Chromosome Stability in Brassica Napus Resynt-Hesised by Protoplast Fusion....Pages 177-178
Involvement of Protoplast Fusion in Cauliflower Breeding....Pages 179-180
Selection of a Universal Hybridizer in Sinapis Turgida Del. and Regeneration of Plantlets from Somatic Hybrids with Brassica Species....Pages 181-182
Towards the Introduction of Cytoplasmic Male Sterility (CMS) into Brassica Napus Through Protoplast Fusion....Pages 183-184
Somatic Hybridization of Diploid Potato Lines, a Tool in Potato Breeding....Pages 185-186
Somatic Hybridization between Anther-Derived Dihaploid Lines of Solanum Tuberosum L. (Potato)....Pages 187-188
Fusion of Dihaploid Solanum Tuberosum Protoplasts and Trials of Hybrid Selection with Phytotoxins....Pages 189-190
Fusion of Solanum tuberosum L. cv. Explorer(Potato) and Solanum melongena L. cv. Imperial Black Beauty(Eggplant) Protoplasts and Subsequent Callus Regeneration....Pages 191-194
“Somatic Fusions of Solanum Species”....Pages 195-196
Flow Cytometric Sorting of Potato Heterokaryons with Inhibition of Cell Wall Synthesis before Sorting....Pages 197-198
An Improved Peg Fusion Procedure for Flow Sorting of Plant Protoplasts....Pages 199-200
Interspecific Expression of AEC-Resistance in Somatic Hybrids of AEC-Resistant Solanum Tuberosum and Nitrate-Reductase-Deficient Nicotiana Plumbaginifolia....Pages 201-201
Interspecific Protoplast Fusion for the Transfer of Atrazine Resistance from Solanum Nigrum to Tomato (Lycopersicon Esculentum.L)....Pages 203-204
Somatic hybridization between tomato and other Lycopersicon or Solanum species....Pages 205-207
Transfer of Regeneration Capacity from Lycopersicon Peruvianum to L. Esculentum by Protoplast Fusion....Pages 209-210
Somatic Hybridization in Lycopersicon; Using Gamma-Irradiated Nicotiana Tabacum SR-1 or Lycopersicon Peruvianum....Pages 211-212
Regeneration of Somatic Hybrid Plants between Tobacco (Nicotiana Tabacum L.) and Black Nightshade (Solanum Nigrum L.)....Pages 213-214
Intra- and Interspecific Gametosomatic Hybridisation within the Genus Petunia....Pages 215-218
The Transfer of Cytoplasmic Male Sterility (CMS) by Intraspecific Somatic Hybridisation in Petunia Hybrida (Hort) VILM....Pages 219-220
Application of Protoplast Fusion to an Ornamental Breeding Programme....Pages 221-224
Formation of Carrot Cybrids Based on Metabolic Complementation by Fusion between X-Irradiated Cells and Iodoacetamide-Treated Cells....Pages 225-226
Optimization of Conditions for Somatic Cybridization of Daucus Carota L....Pages 227-230
Isolation and Fusion of Apium Graveolens (Celery) and Levisticum Officinale (Lovage) Mesophyll Protoplasts....Pages 231-232
Electrofusion of Plant and Fungal Protoplasts....Pages 233-234
Interspectfic Hybrid Lines of M. Sativa and M. Arborea by Protoplast Electrofusion....Pages 235-236
Electrofusion and Electroporation of Sugar Beet (Beta Vulgaris L.) Protoplasts....Pages 237-240
The Production and Electrofusion of Beta Cytoplasts....Pages 241-242
Inactivation of Sugarbeet Protoplasts Using Acridin Orange, an Agent for Late Selection of Fusion Products....Pages 243-244
Mitochondrial DNA Amplification in Somatic Hybrid Cells of Grasses....Pages 245-248
Electrofusion of Chemically-Aggregated Protoplasts Derived from Mesophyll Tissue and Habituated Cells of Catharanthus Roseus....Pages 249-250
Studies on the Transfer of the Mitochondrial Genome Through Protoplast Fusion in Rice....Pages 251-252
Increase of Genetic Variability by Asymmetric Cell Hybridization and Isolated Chromosome Transfer....Pages 253-254
Cell-Mediated Gene Transfer....Pages 255-256
Induction of Micronuclei by Amiprophos-Methyl and their Application for Transfer of Single, Specific Chromosomes in Plant Cells....Pages 257-258
Isolation of Microprotoplasts....Pages 259-260
Direct Cell to Cell Transfer of Mitochondria by Microinjection....Pages 261-262
Classification and Sorting of Plant Chromosomes Using Flow Cytometry....Pages 263-264
Electroporation for DNA Delivery to Plant Protoplasts....Pages 265-266
Electromanipulation of Protoplasts....Pages 267-268
Plant Protoplast Electropermeabilization: Role of Electrical and Biological Parameters....Pages 269-270
The Fusogenic State of Plant Protoplasts as Related to DYE/DNA Uptake....Pages 271-272
Genetic Manipulation of Plant Cells and Organelles with a Laser Microbeam....Pages 273-274
Electric Field Mediated DNA Uptake and Gene Expression in Mesophyll, Suspension Culture, and Tuber Protoplasts of Wild and Cultivated Potato....Pages 275-281
Introduction of Selectable Markers into Different Diploid Potato (Solanum Tuberosum L.) Genotypes by Direct Gene Transfer....Pages 283-285
Direct Gene Transfer in Petunia Hybrida Protoplasts: Evidence for Co-Transformation....Pages 287-288
Electroporation of Tobacco Protoplasts: A Model System to Study Plant Gene Expression....Pages 289-292
Infection of Tobacco Protoplasts with RNA of a Pepper Strain of Tobacco Mosaic Virus by Electroporation....Pages 293-296
Comparison of Direct Gene Transfer into Nicotiana Protoplasts by Electroporation and Mg2+/PEG Methods....Pages 297-298
Quantification of Transient Expression Levels of Genes Transferred to Plant Protoplasts by Electroporation....Pages 299-300
Gene Transfer by Electroporation in Alnus Incana Protoplast....Pages 301-302
Transformation of Rice Protoplasts by Direct DNA Uptake....Pages 303-304
Isolation, Culture and Morphogenesis from Wheat Protoplasts, and Study of Expression of DNA Constructs by Direct Gene Transfer....Pages 305-310
UV Inducible Transient Expression of Chimeric CHS-NPTII in Parsley Proto — Plasts....Pages 311-314
Modification of Plants Using Intracellular Associations of L-Form Bacteria....Pages 315-316
Transformation of Brassica Nigra Through Protoplast Cocultivation with Agrobacterium Tumefaciens....Pages 317-318
Transformation of Brassica Species Via Direct Gene Transfer....Pages 319-322
Genetic Transformation of Diploid Solanum Tuberosum by Direct DNA Transfer and Mediated by Agrobacterium Tumefaciens....Pages 323-324
Optimization of Direct Gene Transfer in Tomato....Pages 325-327
Transformation Studies in Tobacco, Moth Bean and Brassica Species....Pages 329-330
Transposition and Regulation of the En Elements of Maize Assayed in Transformed Tobacco....Pages 331-332
Initiation of Cell Suspensions, Protoplast Culture, and Direct Gene Transfer to Protoplasts of Secale cereale L. (rye)....Pages 333-334
Protoplast Culture and DNA Uptake in Echinocloa....Pages 335-336
Transient Expression of Foreign Genes in Wheat Protoplasts....Pages 337-338
Protoplast culture and transformation studies of Triticale (x Triticosecale Wittmack)....Pages 339-340
Analysis of the Zea Mays Sucrose Synthase Promotor by Transient Gene Expression in Maize Protoplasts....Pages 341-342
Protoplast Culture of 2n S. Tuberosum as a Source of Mutant Phenotypes....Pages 343-346
Isolation of Temperature-Sensitive Auxin-Requiring Variants of Nicotiana Plumbaginifolia....Pages 347-348
The use of phosphinothricin resistance as a selectable marker in tobacco protoplast transformation....Pages 349-350
Selection for Salt/Drought Tolerance Using Isolated Protoplasts and Protoplast-Derived Calli of Colt Cherry (Prunus Avium X Pseudocerasus)....Pages 351-352
Field Tests of Progeny from Protoplast Regenerated Oilseed Rape....Pages 353-354
Studies of lettuce plants regenerated from protoplasts....Pages 355-356
Somaclonal Variations for Indole Alkaloid Accumulation in Protoclones Derived from an Habituated Strain of Catharanthus Roseus....Pages 357-358
Somaclonal Variations of Dihydrofuroquinoline Alkaloids in Cell Lines Issued from Strains and Leaf Mesophyll of Choisya Ternata by Protoplast Cloning....Pages 359-362
Some Aspects of Salinity Tolerance of Calli Derived from Leaf Tomato Protoplasts....Pages 363-364
Proto-Variation in Protoplast Derived Brassica Napus Plants....Pages 365-367
Back Matter....Pages 369-370
....Pages 371-372