Ebook: Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions

This volume represents a collection of pioneering papers introducing immunochemical techniques to the aquatic sciences. It is the product of a workshop entitled "Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions" held at the University of Southern Maine, Portland, Oct. 5-7, 1986. Funding from many sources made this workshop possible, and is gratefully acknowledged. The reader will note great variability in both the quality and scope of the papers which we have divided 'into four sections: I. Background material on the immune system (borrowed from a recent NIH publication) II. Background material related to aquatic sciences III. Techniques IV. Applications Readers familiar with terminology are directed to start with the original contributions related to aquatic sciences, page 21. Some of the contributions are abstracts only. Others are preliminary, for which we are grateful to the authors for their willingness to share recent sometimes tentative findings with a broader community. Some offer novel and tempting insights, but as they exist, lack adequate standards and controls. Others are thorough and represent major scientific contributions. The promise rests in generating and working with reagents of great specificity enabling orders of magnitude improvements in the sensitivity of our measurements. Authors also address the problems and limitations. We thank the numerous authors and reviewers. In support of every successful endeavor is someone of unusual savvy. For us, this has been David A. Phinney. His leadership, wisdom and wit have been important to the introduction of flow cytometry/sorting and immunochemistry into aquatic research.

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This important new volume provides researchers in marine sciences with an overview of pioneering work that has introduced the use of immunochemical techniques to aquatic sciences. The promise of these techniques rests in the ability to generate and work with reagents of great specificity ensuing improvements in the sensitivity of measurements in the order of magnitudes. In a series of chapters ranging from major scientific contributions to stimulating progress reports, the authors not only summarize their findings but also address the problems and limitations at the current state of the art.

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This important new volume provides researchers in marine sciences with an overview of pioneering work that has introduced the use of immunochemical techniques to aquatic sciences. The promise of these techniques rests in the ability to generate and work with reagents of great specificity ensuing improvements in the sensitivity of measurements in the order of magnitudes. In a series of chapters ranging from major scientific contributions to stimulating progress reports, the authors not only summarize their findings but also address the problems and limitations at the current state of the art.
Content:
Front Matter....Pages N2-XVI
Front Matter....Pages 1-1
Understanding The Immune System....Pages 2-21
Front Matter....Pages 23-23
What Does the Immunological Approach Offer Aquatic Research? An Overview....Pages 24-40
Molecular Biology, Single Cell Analysis and Quantitative Genetics: New Evolutionary Genetic Approaches in Phytoplankton Ecology....Pages 41-71
Front Matter....Pages 73-73
The Production of Monospecific Antisera to Soft-Bottom Benthic Taxa....Pages 74-98
Preparation and use of Monospecific Antisera to Capitella SP. I.....Pages 99-99
Cell Affinity Chromatography for a Marine Nitrifying Bacterium....Pages 100-116
Application of Immunoblotting for Dietary Analysis....Pages 117-129
Two-Dimensional Electrophoresis with Silver Staining: A Sensitive Method For Analyzing Complex Protein Mixtures....Pages 130-133
Brevetoxins and Binding: Sodium Channels Versus Antibodies....Pages 134-143
Preparation of Model Haptens to Express Common Epitopes of PSP Toxins....Pages 145-154
Monoclonal Antibodies (Monoabs) to Ciguatoxin and Related Polyethers....Pages 155-166
Immunofluorescent Reagents: Preparation and Analysis....Pages 167-183
Flow Cytometry and Immunofluorescence in Aquatic Sciences....Pages 184-193
Detection Schemes for Antigen-Antibody Reactions....Pages 194-206
Front Matter....Pages 207-207
Identification of Marine Chroococcoid Cyanobacteria by Immunofluorescence....Pages 208-229
An Immunofluorescence Approach toward the Identification of Endosymbiotic Diatoms in Several Species of Larger Foraminifera....Pages 230-241
Immunofluorescent Characterization of the Eukaryotic Ultraplankton....Pages 242-242
Probes for Assessing Single-Cell Primary Production: Antibodies Against Ribulose-1,5-Bisphosphate Carboxylase (RuBPCase) and Peridinin/Chlorophyll A Protein (PCP)....Pages 243-262
Examining Nitrate Reduction by Phytoplankton with an Immunoassay....Pages 263-276
A Possible Immunological Approach to the Study of Plasmalemma Redox Enzymes in Phytoplankton....Pages 277-277
Front Matter....Pages 207-207
Environmental Induction of Teleost Cytochrome P-450 Detected by Immunochemical Analysis....Pages 278-282
Quantifying Stomach Contents Using Immunoassays: A Critique....Pages 283-290
Euphausiid Predation on Larval Anchovy at Two Contrasting Sites Off California Determined with an Elispot Immunoassay....Pages 291-294
Enzyme Immunoassay Utilizing Monoclonal Antibodies for Identification of European Eel Virus (EEV), an Aquatic Birnavirus....Pages 295-303
Monoclonal Antibodies to Atlantic Salmon (Salmo Salar) Immunoglobulin: Production and Preliminary Characterization....Pages 304-311
Immunochemical Evaluation of The Infectious Pancreatic Necrosis (IPN) Carrier State in Salmonids and Other Species....Pages 312-317
Back Matter....Pages 318-330
....Pages 331-343