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Ebook: Progress in Photosynthesis Research: Volume 2 Proceedings of the VIIth International Congress on Photosynthesis Providence, Rhode Island, USA, August 10–15, 1986

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These Proceedings comprise the majority of the scientific contributions that were presented at the VIIth International Congress on Photosynthesis. The Congress was held August 10-15 1986 in Providence, Rhode Island, USA on the campus of Brown University, and was the first in the series to be held on the North American continent. Despite the greater average travel distances involved the Congress was attended by over 1000 active participants of whom 25% were registered students. This was gratifying and indicated that photosynthesis will be well served by excellent young scientists in the future. As was the case for the VIth International Congress held in Brussels, articles for these Proceedings were delivered camera ready to expedite rapid publication. In editing the volumes it was interesting to reflect on the impact that the recent advances in structure and molecular biology had in this Congress. It is clear that cognizance of structure and molecular genetics will be even more necessary in the design of experiments and the direction of future research.








Content:
Front Matter....Pages I-XXVII
Structural Principles of the Antenna System of Photosynthetic Organisms....Pages 1-8
The Molecular Size of Photosynthetic Membrane Protein Complexes....Pages 9-11
Structural Studies on the Light-Harvesting Polypeptides of RP. Acidophila....Pages 13-16
Localization and Comparative Chemical Activity of SH-Groups of the Subunits in the Reaction Centers from Rhodopseudomonas Sphaeroides R-26....Pages 17-19
Studies of Chloroplast Photosystem I Using Monoclonal Antibodies....Pages 21-24
Bound Acceptors of Photosystem I....Pages 25-28
Photosystem I Complex of Higher Plants: Purification and Characterization of its Two Chlorophyll-Protein Complexes....Pages 29-32
Preparation and Properties of Photosystem-1 Reaction Center Complex....Pages 33-36
The Organization of the Fe-S Acceptors of Photosystem 1....Pages 37-40
Analysis of Isolated PS I Polypeptides for Acid Labile Sulfide....Pages 41-44
Light-Harvesting Chlorophyll-Proteins of Barley Photosystem I....Pages 45-48
Measurement of the Optical Cross Section of Photosystem I in Chlorella....Pages 49-52
Isolation of a Photosystem II Reaction Center Consisting of ? and ? Subunits (D-1 and D-2) and Cytochrome b-559....Pages 53-56
Quantitation of Plastoquinone and Functional Electron Carriers in the Photosystem II Reaction Center Complex....Pages 57-60
Identification of QA-, QB- and Manganese-Carrying Subunit Polypeptides of Synechococcus Photosystem II Reaction Center Complex....Pages 61-64
The Organisation of Photosystem II Chlorophyll-Proteins....Pages 65-68
Phosphorylated Polypeptides of the Photosystem 2 Core Complex....Pages 69-72
Organization of Photosystem II Core and Light-Harvesting Complexes....Pages 73-76
Chemical Composition of Photosystem II Reaction Centers (PSII); Phosphorylation of PSII Polypeptides....Pages 77-80
Topology of Polypeptides and Redox Components in PS II and the Cytochrome b/f-Complex....Pages 81-88
Does the Rapidly Metabolized 32 kDa Protein Carry the Reaction Center of PS II?....Pages 89-92
Analysis of Photosystem II Proteins by Two-Dimensional Electrophoresis....Pages 93-96
The 5 kDa, 10 kDa and 22 kDa Polypeptides of Higher Plant Photosystem II....Pages 97-100
Low Molecular Weight Polypeptides in Photosystem II and Protein Dependent Acceptor Requirement for Photosystem II....Pages 101-104
Characterization of a Monoclonal Antibody which Reacts with the 49 kDa Polypeptide of Photosystem II from Spinach....Pages 105-108
Monoclonal Antibodies to the Light Harvesting Chlorophyll-Protein Complex....Pages 109-112
Isolation and Immunological Characterization of a Carotenoid-Binding Protein from the Cyanobacteria Synechocystis Sp. PCC6714 and Anacystis Nidulans R2....Pages 113-116
Isolation and Characterization of A Carotenoid-Associated Membrane Protein from The Cyanobacterium, Anacystis Nidulans R2....Pages 117-120
Structural Principles of Cryptomonad Biliproteins and Cyanobacterial Phycobiliproteins....Pages 121-124
Non-Hemidiscoidal Phycobilisome in Cyanophytes....Pages 125-128
Comparative Immunology of the Phycobilisome Linker Polypeptides....Pages 129-132
Structural Studies on Cytochrome b6 and the Rieske FeS-Center....Pages 133-136
Isolation and Characterization of the Qbc -Complex from the Thermophilic Cyanobacterium Synechococcus 6716....Pages 137-140
Photosynthetic Pigment Organization in Membranes of Eucartotic Plants....Pages 141-144
Pigment-Protein Complexes and Light Harvesting in Eustigmatophyte Algae....Pages 145-148
Immunological Studies on the Chlorophyll a+b Antenna Complexes of Photosystem I and Photosystem II....Pages 149-152
Large-Scale Preparation of Highly Purified Chloroplast Lipids for Liposome Permeability Studies....Pages 153-156
The Association between Galactolipids and Chlorophyll-Protein Complexes....Pages 157-160
Characterization of Galactosyltransferases in Spinach Chloroplast Envelope Membranes. UDPgal-Dependent and -Independent Galactolipid Synthesis....Pages 161-164
Effects of Monogalactosyldiacylglycerol (MGDG) Depletion on Photosynthetic Activities in OAT Thylakoid Membranes....Pages 165-172
Galactolipids and Thylakoid Intramembrane Particles....Pages 173-176
Reconstitution of Functional Phycobilisome-Thylakoid Association in Cyanobacteria....Pages 177-180
Molecular Organization of the Inner Etioplast Membranes....Pages 181-184
Surface Properties of the Prolamellar Body Membrane....Pages 185-188
P700 Spectra and Concentrations in Several Plants and a Cyanobacterium....Pages 189-192
Recovery Time of Physiologically Inactive Photosystem II Reaction Centers Measured by the Electrochromic Absorbance Change at 515nm....Pages 193-196
Identification of Photosystem II Centers Inactive in Plastoquinone Reduction....Pages 197-200
Photosystem Stoichiometry in Higher Plant Chloroplasts....Pages 201-204
Cause and Effect Relationship Between Environmental Conditions and Photosystem Stoichiometry in Synechococcus 6301....Pages 205-208
Differential Phosphorylation of Thylakoid Membrane Polypeptides During Chloroplast Adaptation to Light Quality....Pages 209-212
The Physiological Significance of Thylakoid Membrane Protein Phosphorylation....Pages 213-216
Development of The Light-Harvesting Antenna of the Photosystems in Chlorophyll-b Deficient Mutants....Pages 217-220
Comparative Studies on the Light Absorption Capacity of Photosystem II and Photosystem I....Pages 221-224
An Analysis of the Salt-Induced Rise of Chlorophyll Fluorescence to Investigate Ion-Specific Effects Between and within Valency Groups....Pages 225-228
Differential Thylakoid Membrane Stacking in Mangrove and Spinach....Pages 229-232
The Role of LHCII in Thylakoid Membranes....Pages 233-236
Subfractionation of Inside-Out Thylakoid Vesicles-Preparation of Pure Photosystem II Particles without using Detergent....Pages 237-240
Composition of the Marginal Regions of Thylakoid Membranes....Pages 241-244
Effect of Divalent Cations on the Aging of Wheat Chloroplasts....Pages 245-248
Cytochrome F Exists as Two Functionally Separate Pools in Pea Thylakoid Membranes....Pages 249-252
Structural Identification of the Oxygen-Evolving Apparatus on the Lumenal Surface of Freeze-Etched Thylakoid Membranes....Pages 253-256
Lateral Distribution of the Photosystem I Complex between the Appressed and Non-Appressed Regions of Spinach Thylakoid Membranes: An Immunocytochemical Study....Pages 257-260
Differential Solubilization of the Integral Electron Transport Complexes from the Thylakoid Membrane of Spinach Chloroplasts. Localization of Photosystem I, Photosystem II and the Cytochrome B6-F Complex....Pages 261-264
Immunogold Localization of the Phycobiliprotein of a Cryptophyte Alga to the Intrathylakoidal Space....Pages 265-268
Immunogold Labeling of Thylakoid Membranes....Pages 269-272
Assignments of Stroma-Exposed PSII Proteins after Two-Dimensional Electrophoresis using Affinity Purified Polyclonal Antibodies....Pages 273-276
Determination of the Insideout to Rightsideout Ratio of: 1) Thylakoids Exposed to Phase Partition, 2) Thylakoids Washed with Edta, and 3) Detergent Derived PSII Membranes....Pages 277-280
Localization of the b6/f Complex By Freeze-Fracture Analysis of the Chlamy-Pomonas Reinhardtii and Chlorella Sorokiniana Mutants Lacking in this Complex....Pages 281-284
Immunocytochemical Studies on the Organization of Thylakoid Membrane Proteins....Pages 285-288
Collapse Mechanism of some Carotenoid Monomolecular Films - Membrane Model....Pages 289-292
Adaptation of Barley Thylakoid Membranes to Changing Light Intensity....Pages 293-296
Pigment-Protein Complexes of Algal Thylakoid Membranes: Variations in Pattern, Pigment Composition and Reaction Centre II Types During The Cell Cycle of Chlorella Fusca and after Adaptation to Low Light Intensities....Pages 297-300
On the Protective Role of Carotenoids in the Ps I Reaction Centre and LHCI Complexes of The Thylakoid Membrane....Pages 301-304
Response of Chlamydomonas Reinhardtii Reaction Center Stoichiometry to Light Intensity Variation: A Spectroscopic and Lds-Page Analysis....Pages 305-308
Effects of Nitrate Nutrition on Chloroplast Composition and Photosynthetic Activities in Wheat....Pages 309-312
The Effect of Different Growth Light Intensities On Photosystem II Components....Pages 313-315
Changes in Pool Sizes of Photosynthetic Electron Transport Components and Rubisco with Growth Irradiance - Evidence for the Limiting Step in Light Saturated Photosynthesis....Pages 317-320
Chlorophyll-Protein Complexes Changes Associated with Chloroplast Development in a Virescent Petunia Hybrida Mutant....Pages 321-324
Altered Photosynthetic Pigment Complements in Mutants of the Cyanobacterium Synechococcus Sp. Spp 7002....Pages 325-328
Characterization of a Novel Chlorophyll-Binding Component of Cyanobacterial Thylakoids....Pages 329-332
Some Aspects of Altered Structure and Functioning of the Photosynthetic Apparatus in Phytochrome-Less Mutants of Tomato....Pages 333-337
Stigmatellin and Other Electron Transfer Inhibitors as Probes for the QB Binding Site in the Reaction Center of Photosynthetic Bacteria....Pages 339-342
Reconstitution of Subunits LM of the Reaction Center from Rhodopseudomonas Sphaeroides into Liposomes....Pages 343-346
Covalent Binding of Halogen Substituted 1,4-Quinones to Proteins of the Thylakoid Membrane....Pages 347-350
Competition of Inhibitors with The Secondary Quinone in Dark Adapted Thylakoid Membranes....Pages 351-354
Kinetics of the Changes in Oxidation-Reduction States of the Acceptors and Donors of Photosystem II in Pea Thylakiods Measured by Flash Fluorescence....Pages 355-358
The Effect of pH and Flash Frequency on Electron Transfer Through the Quinone Acceptor Complex of Ps II in Bicarbonate Depleted or Anion Inhibited Thylakoid Membranes....Pages 359-362
Manipulations of the Pool Characteristics of the Quinone Electron Donor and Acceptor to Ubiquinol-Cytochrome c Oxidoreductase. Evidence for Redox Contact between the Cytochrome b Hemes....Pages 363-366
Inhibition of Electron Transfer in the Cytochrome b/f Complex By NQNO and DBMIB: Evidence for Two Quinone Binding Sites....Pages 367-370
The Electrogenic Reaction and Proton Release During Quinol Oxidation by the Cytochrome b/F Complex....Pages 371-374
Electron and Proton Transfer in The Cytochrome bf Complex....Pages 375-377
The Effect of Pre-Reduction of the b-type Cytochromes on Electron Transport Through the Cytochrome bf Complex in Chloroplasts....Pages 379-382
The Plastoquinone/Plastoquinol Oxidoreductase Sites of the Chloroplast Cytochrome b/f Complex....Pages 383-386
Proposed Alignment of Amino Acid Sequences for Cytochromes c1 and f and Identification of Putative Binding Sites for Cytochrome c and Plastocyanin....Pages 387-396
Quinol Specificity of the Cytochrome b6f-Complex - Evidence against a Permanently Bound Plastoquinone Molecule....Pages 397-400
The Antimycin-binding Site of Thylakoid Membranes from Chloroplasts....Pages 401-404
Inhibition of High Energy State Quenching in Spinach Chloroplasts by Low Concentrations of Antimycin A....Pages 405-408
Plastoquinol and Plastoquinone Diffusion in Model Membranes....Pages 409-412
Temperature Dependence of DF-Kinetics and PQ-conductivity....Pages 413-416
A Carbon-13 NMR Study of Plastoquinone in Membranes....Pages 417-419
Lateral Electron Transport in Thylakoids of Higher Plants....Pages 421-424
On the Electron Transfer from Plastocyanin to P700....Pages 425-428
Use of Affinity Chromatography to Purify Plastocyanin and Cytochrome f....Pages 429-432
The Effect of Ethylenediamine Chemical Modification on the Electron Transport Properties and Redox Potential of Spinach Plastocyanin....Pages 433-436
The Effect of pH on the near UV Circular Dichroic Spectra of Parsley Plastocyanin....Pages 437-440
Ferredoxin-Dependent Cyclic Electron Transport and Photophosphorylation....Pages 441-444
Cyclic Electron Transport Around Photosystem I in Washed Thylakoids....Pages 445-452
Ferredoxin-NADP+ Oxidoreductase: Studies on the Thylakoid Membrane Bound Enzyme....Pages 453-460
Inhibitors of Ferredoxin-Catalysed Cyclic Photphosphorylation in Chloroplasts....Pages 461-464
Photosynthetic Control of Electron Transport in Leaves of Phaseolus Vulgaris: Evidence for Regulation of Photosystem 2 by the Proton Gradient....Pages 465-468
Analysis of the ? and ? Centres of Photosystem II, in Presence of Atrazine and TMPD....Pages 469-472
Senescence of Chloroplasts in Fern Leaves....Pages 473-476
The Role of Cu in the Regulation of the Photosynthetic Electron Flow....Pages 477-480
Copper in Photosystem II....Pages 481-484
Inhibition of the Photosynthetic Electron Transport of Isolated Chloroplasts by Hemolyzed Rabbit Sera....Pages 485-488
Effect of Dimethylaminoethyl p-Chlorophenoxyacetate (Centro-Phenoxine) on Chloroplast Electron Transport Activity....Pages 489-492
Effect of Resorcinols on Electron Transport in Pea Chloroplasts....Pages 493-496
Ascorbate As A Pro-Oxidant and an Anti-Oxidant During a Mehler Reaction....Pages 497-500
Effects of Deuterium Oxide on Photosynthetic Light Reactions....Pages 501-504
Senescence and Heat Stress Induced Alterations of Photosynthetic Electron Transport....Pages 505-508
Oscillation in Photosystem II Activity of Chloroplasts Isolated from Dark Adapted Wheat Seedlings....Pages 509-512
Diurnal Changes in Electron Transport Capacity in Pea Thylakoids....Pages 513-520
Modelling of Photosynthetic Energy Conversion I Description and Basic Principles of the Model....Pages 521-524
Modelling of Photosynthetic Energy Conversion. II. Charge Separation and Secondary Electron Flow....Pages 525-528
Modelling of Photosynthetic Energy Conversion III. Electrochemical Events at the Membrane....Pages 529-532
Photoacoustic Action Spectra of Bundle-Sheath Cells of Zea Mays....Pages 533-536
Photoacoustic Study of Photosynthetic Activity in Bundle-Sheath Cells of Zea Mays....Pages 537-540
Sulfide-Dependent Electron Transport in Thylakoids from the Cyanobacterium Oscillatoria Limnetica....Pages 541-544
Energetics of Hydrogen Recycling in Nitrogen-Fixing Cyanobacteria....Pages 545-548
Photosynthesis, Nitrogen Fixation, Ammonia Photoproduction and Structure of Anabaena Azollae Immobilized in Natural and Artificial Systems....Pages 549-552
Sustained Ammonia Production by Immobilized Cyanobacteria....Pages 553-556
Cellular Biochemical Activities and Induction of Synchronous Growth in a Unicellular Aerobic Nitrogen-Fixing Cyanobacterium, Synechococcus Sp. Strain Miami BG 043511....Pages 557-560
Inhibition of Respiration By Short Saturating Flashes in Chlamydomonas: Evidence for a Chlororespiration....Pages 561-564
Studies on the Linking of Photosynthetic and Respiratory Ellectron Transport Chains and Phosphorylation....Pages 565-568
A Portable Double-Flash Spectrophotometer for Measuring the Kinetics of Electron Transport Components in Intact Leaves....Pages 569-572
Dynamic Aspects on the Organization of the Thylakoid Membrane....Pages 573-576
Different Polypeptide Composition of the Peripheral and Tightly Bound Subpopulations of LHCII....Pages 577-580
Interplay between Environmental and Metabolic Factors in the Regulation of Electron Transport in Higher Plants....Pages 581-584
The Importance of Membrane Surface Electrical Charge on the Regulation of Photosynthetic Electron Transport by Reversible Protein Phosphorylation....Pages 585-587
A Light-Sensitive Photoregulatory Mutant in Maize Deficient in LHCI and the ‘Mobile’ Chlorophyll a/b LHCII....Pages 589-592
Immunolocalization of the CHL a/b-Light Harvesting Complex and CP29 under Conditions Favoring Phosphorylation and Dephosphorylation of Thylakoid Membranes (State 1- State 2 Transitions)....Pages 593-596
Comparison between The Effects of Cations, State I-State II Transitions and Protein-Phosphorylation on Lifetime Components of Chlorophyll Fluorescence....Pages 597-604
Does a Correlation between the Xanthophyll Cycle and State 1 -State 2 Changes Exist?....Pages 605-608
Altered Lipid Matrix Changes Energy Distribution in Thylakoids....Pages 609-612
Energy Pipeline Model of the Photosynthetic Apparatus....Pages 613-616
Influence of Thylakoid Protein Phosphorylation on PS I Dependent Photophosphorylation and on Electron Transport....Pages 617-620
The Kinetics of Phosphorylation of the Light Harvesting Chlorophyll a/b Protein and Associated Changes in Photosystem Cross Section and Energy Spillover....Pages 621-624
Reversal Movement of Sub-Population of Light Harvesting Chlorophyll Protein Complexes LHC-II and LHC-I between Grana and Stroma Lamellae under Different Conditions....Pages 625-628
A possible redistribution of excitation energy in spinach chloroplasts due to nitrite ions....Pages 629-632
Multiple Effects of Trypsin- and Heat-Treatments on the Ultrastructure and Surface Charge Density of Pea Chloroplast Membranes. Influence on P700+ Parameters....Pages 633-636
Steady-State Kinetics of Chlorophyll-Fluorescence and Light Scattering....Pages 637-640
Extreme Changes in the Distribution of Excitation Energy in the Photochemical Apparatus of Intact Leaves Induced by Progressive Heat Stress....Pages 641-644
Environmental Control of Photosynthetic Enhancement in Palisade Mesophyll Chloroplasts Measured by Photoacoustic Spectroscopy....Pages 645-648
Regulation of Photosynthetic Unit Function by Protein Phosphorylation....Pages 649-652
The 18.5 kDa Phosphoprotein of the Cyanobacterium Synechococcus 6301 : A Component of the Phycobilisome....Pages 653-656
State 1-State 2 Transitions in Synechococcus 6301 are Influenced by Respiratory Electron Flow; Evidence for the Control of Excitation Energy Distribution by the Redox Level of Plastoquinone in Cyanobacteria....Pages 657-660
Control of State 1 - State 2 Transitions in the Blue-Green Alga Nostoc Muscorum....Pages 661-664
The Relationships between Protein Kinase Activity and Chlorophyll a Fluorescence Changes in Thylakoids from the Cyanobacterium Synechococcus 6301....Pages 665-668
Excitation Energy Transfer in Cryptomonas Ovata. Preillumination Dependent Changees in 77K Picosecond Time Resolved Fluorescence Emission Spectra....Pages 669-676
Effect of Growth Temperature on Membrane Properties in a Thermophilic Cyanobacterium (Synechococcus Sp.)....Pages 677-680
Increased Energy Transfer from the Phycobilisomes to Photosystem II in High Light Adapted Anabaena Cylindrica....Pages 681-688
Purification and Characterization of a Thylakoid Protein Kinase....Pages 689-696
Reconstitution of LHC Phosphorylation by a Protein Kinase Isolated from Spinach Thylakoids....Pages 697-700
The Sensitivity of Pisum Sativum Thylakoid Membrane Protein Kinase Activity to Inhibitors, Detergents and Heating....Pages 701-704
Back Matter....Pages 705-708
....Pages 709-712
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