Ebook: Structure, Function, and Genetics of Ribosomes

During the past few decades we have witnessed an era of remarkable growth in the field of molecular biology. In 1950 very little was known of the chemical constitution of biological systems, the manner in which information was transmitted from one organism to another, or the extent to which the chemical basis of life is unified. The picture today is dramati­ cally different. We have an almost bewildering variety of information de­ tailing many different aspects of life at the molecular level. These great advances have brought with them some breath-taking insights into the molecular mechanisms used by nature for replicating, distributing, and modifying biological information. We have learned a great deal about the chemical and physical nature of the macromolecular nucleic acids and proteins, and the manner in which carbohydrates, lipids, and smaller mole­ cules work together to provide the molecular setting of living systems. It might be said that these few decades have replaced a near vacuum of information with a very large surplus. It is in the context of this flood of information that this series of mono­ graphs on molecular biology has been organized. The idea is to bring together in one place, between the covers of one book, a concise assessment of the state of the subject in a well-defined field.

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Content:
Front Matter....Pages i-xxii
Structure of Ribosomes....Pages 1-27
Immuno Electron Microscopy on Escherichia coli Ribosomes....Pages 28-46
Ribosome Structure, Function, and Evolution: Mapping Ribosomal RNA, Proteins, and Functional Sites in Three Dimensions....Pages 47-67
Structure of Ribosomes and Their Components by Advanced Techniques of Electron Microscopy and Computer Image Analysis....Pages 68-86
Neutron-Scattering Analysis of Structural and Functional Aspects of the Ribosome: The Strategy of the Glassy Ribosome....Pages 87-100
Studies on Crystals of Intact Bacterial Ribosomal Particles....Pages 101-111
Self-Organization of Ribosomal RNA....Pages 112-127
Studies on the Structure and Function of Ribosomal RNA....Pages 128-142
Comparative Structural and Functional Studies on Small Ribosomal RNAs....Pages 143-163
RNA Structure and RNA-Protein Neighborhoods in the Ribosome....Pages 164-183
RNA Structure in Solution and on the 30S Ribosomal Subunit....Pages 184-202
The Folding of RNA in the 30S Subunit of Escherichia coli Ribosomes....Pages 203-216
Probing Ribosomal Structure and Function....Pages 217-232
How Do Protein L18 and 5S RNA Interact?....Pages 233-252
Structure and Interactions of Ribosomal Components and Ligands....Pages 253-269
Protein Topography of Ribosomal Functional Domains: Effects of Monoclonal Antibodies to Different Epitopes in Escherichia coli Protein L7/L12 on Ribosome Function and Structure....Pages 270-285
On the Structure, Function, and Dynamics of L7/L12 from Escherichia coliRibosomes....Pages 286-308
Ribosomal Proteins: Their Structure and Evolution....Pages 309-325
Photoaffinity Labeling of Escherichia coli Ribosomes: New Approaches and Results....Pages 326-361
Structural Studies of the Factor Binding Domain....Pages 362-378
Studies of the Structure of Eukaryotic (Mammalian) Ribosomes....Pages 379-390
Structural Dynamics of the Translating Ribosome....Pages 391-411
Conformational Change of 50S Ribosomes during Protein Synthesis....Pages 412-424
Three tRNA Binding Sites Involved in the Ribosomal Elongation Cycle....Pages 425-437
Structural and Functional Interactions of the tRNA-Ribosome Complex....Pages 438-453
The Movement of tRNA through Ribosomes during Peptide Elongation: The Displacement Reaction Model....Pages 454-472
Isomers of Aminoacyl- and Peptidyl-tRNA in the Peptidyl Transferase Reaction....Pages 473-494
Mechanism of Ribosomal Translocation....Pages 495-508
The Aminoacyl-tRNA·Elongation Factor Tu·GTP Ternary Complex and Its Role in Aminoacyl-tRNA Selection at the Ribosome....Pages 509-522
Stereochemical Analysis of Ribosomal Transpeptidation, Translocation, and Nascent Peptide Folding....Pages 523-540
Ribosomal Proteins Tune Rate and Accuracy in Translation....Pages 541-555
Involvement of Specific Portions of Ribosomal RNA in Defined Ribosomal Functions: A Study Utilizing Antibiotics....Pages 556-572
Inhibitors of Protein Synthesis as Phylogenetic Markers....Pages 573-585
Role of the Initiation Factors in Escherichia coli Translational Initiation....Pages 586-604
Arrangement of Eukaryotic Initiation Factor 3 and Messenger RNA within Preinitiation Complexes....Pages 605-620
Novel Functions of EF-Tu during Polypeptide Synthesis and tuf Gene Expression....Pages 621-641
Site-Directed Mutagenesis of Escherichia coli Ribosomal RNA....Pages 642-657
Structure and Expression of Ribosomal Protein Genes in Yeast....Pages 658-671
The Ribosomal Genes of Yeast and Their Regulation....Pages 672-685
Mutant Studies on the Prokaryotic Ribosome....Pages 686-698
Regulation of the Escherichia coli L10 Operon....Pages 699-718
Back Matter....Pages 719-732
....Pages 733-748