Ebook: Methods in Porphyrin Photosensitization

The use of porphyrins for localization and photodynamic therapy of neoplastic disease has been the topic of several international symposia, reviews and books during the preceeding five years. The literature on this topic has continued to grow, as have numbers of presentations at national and international meetings relating to photobiology, chemistry, and lasers. In this volume, it is the intention of the editor to provide both information on current research projects, and detailed methodology used in such investigations. A bibliography on the subjects of porphyrin localization and therapy is included. The manuscripts contained in this volume are based on reports given at a Porphyrin Photosensitization Workshop which was held in Philadel­ phia, July 6-7, 1984. This Workshop was supported by NIH grant CA 36746, together with funds from the Fogarty International Center. Authors were requested to update their contributions to provide a summary of progress to mid 1985. Manuscripts containing material not presented in Philadelphia are also included, notably a series of articles describ­ ing current clinical and pre-clinical results from China. Since the Philadelphia Workshop, a meeting was held in Alghero, Sardinia (May, 1985), and additional conferences are now being planned; this attests to the continued interest in photodynamic therapy involving porphyrin photo­ sensitization.

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Content:
Front Matter....Pages i-ix
Hematoporphyrin Photodynamic Therapy for Transitional Cell Carcinoma of the Bladder — An Update....Pages 3-12
Photodynamic Therapy in the Management of Cancer: An Analysis of 114 Cases....Pages 13-19
Preliminary Results of Hematoporphyrin Derivative-Laser Treatment for 13 Cases of Early Esophageal Carcinoma....Pages 21-25
Optimalization of the Depth of Selective Response of Neoplastic Tissue....Pages 27-34
Dosimetry Methods in Photoradiation Therapy....Pages 35-41
Fluorescence Diagnosis of Cancer....Pages 43-50
Photodynamic Effects And Hyperthermia In Vitro....Pages 51-67
Preliminary Studies with Implanted Polyvinyl Alcohol Sponges as a Model for Studying the Role of Neointerstitial and Neovascular Compartments of Tumors in the Localization, Retention and Photodynamic Effects of Photosensitizers....Pages 69-76
Quantatiye Studies of Tetraphenytlporphinesulfonate and Hematoporphyrin Derivative Distribution in Animal Tumor Systems....Pages 77-89
Correlation Of Tumor Blood Flow To Tumor Regression After Hematoporphyrin Derivative (HPD) Photodynamic Therapy To Transplantable Bladder Tumors....Pages 91-96
The Biological Effects of Photodynamic Therapy on Normal Skin in Mice-I. A Light Microscopic Study....Pages 97-103
The Biological Effects of Photodynamic Therapy on Normal Skin in Mice-II. An Electron Microscopic Study....Pages 105-109
Studies on Hematoporphyrin-Photosensitized Effects on Human Cancer Cells in Vitro: Tem and Sem Observations....Pages 111-115
Photodynamic Effect of Hematoporphyrin on Human Cancer Cells and Normal Diploid Fibroblasts in Vitro....Pages 117-122
Phototoxic Effects of Hematoporphyrin Derivative and its Chromatographic Fractions on Hormone-Producing Human Malignant Trophoblast Cells in Vitro....Pages 123-128
Examination of Potentially Lethal Damage in Cells Treated with Hematoporphyrin Derivative and Red Light....Pages 129-145
Porphyrin Photosensitization of Bacteria....Pages 147-153
Photosensitization of Mitochondrial Adenosine-Triphosphatase and Adenylate Kinase by Hematoporphyrin Derivative in Vitro....Pages 155-159
Porphyrin-Membrane Interactions: Structural, Kinetic and Thermodynamic Aspects Studied Using Fluorescence Techniques....Pages 161-167
Photophysics and Photochemistry of Hematoporphyrin, Hematoporphyrin Derivative and Uroporphyrin I....Pages 169-180
Binding of Porphyrins to Serum Proteins....Pages 181-192
Separation of Different Fractions of Hematoporphyrin Derivative by a Two-Phase Extraction System....Pages 193-205
Chemical, Biologic and Biophysical Studies on ‘Hematoporphyrin Derivative’....Pages 207-211
Some Preparations and Properties of Porphyrins....Pages 213-227
The Structure of Protoporphyrin and of the Aggregate Fraction of Haematoporphyrin Derivative in Solution....Pages 229-266
Chemical Manipulation of the Vinyl Groups in Protoporphyrin-IX....Pages 267-276
Aggregation Effects on the Photophysical Properties of Porphyrins in Relation to Mechanisms Involved in Photodynamic Therapy....Pages 277-292
Studies on the Solvophobic Nature of Porphyrin Self Aggregation vs. the Lipophylic Nature of Porphyrin-Membrane Binding....Pages 293-302
Photodynamic Therapy....Pages 303-311
....Pages 313-328