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In 1980, a conference on tissue culture of fruit crops was held at Beltsville to summarize the current status of this technology and to stimulate interest in it among research scientists, students, and commercial producers in the U. S. Interest in that conference and the proceedings from it far exceeded the expectations of the organizing committee. Since that time, micropropagation of fruit crops in the U. S. has increased significantly, but still lags far behind applications to production of ornamental plants. Within the past two years, a number of new laboratories have been established and some of the existing laboratories have expanded to a size far larger than any previously anticipated. Creation of new laboratories capable of producing more than 400,000 plants per week will test the ingenuity of laboratory managers and the skills of marketing departments. In recent years, numerous symposia have been held on various aspects of biotechnology and genetic engineering. Although micro propagation is the key to providing large numbers of genetically engineered plants, it is a topic that has been relegated to a minor position, or ignored completely, at such meetings. Accordingly, the time seemed propitious for a conference devoted solely to all aspects of micropropagation as applicable to horticultural crops.








Content:
Front Matter....Pages i-x
Principles of Plant Tissue Culture....Pages 1-13
The Application of Tissue Culture to Plant Improvement and Propagation in the Ornamental Horticulture Industry....Pages 15-33
New Technologies in Plant Tissue Culture....Pages 35-51
Plug Systems for Micropropagules....Pages 53-60
Totipotent Suspensions as a Means of Multiplication....Pages 61-72
Phenotypic and Genotypic Stability of Tissue Cultured Plants....Pages 73-94
Summary of Panel Discussion on Phenotypic and Genotypic Stability of Tissue Cultured Plants....Pages 95-96
Pathogen Detection and Elimination....Pages 97-117
Production of Culture Virus-Indexed Geraniums....Pages 119-123
Diagnosis of Plant Viruses Using Double-Stranded RNA....Pages 125-134
Pathogen Indexing in Large-Scale Propagation of Florist Crops....Pages 135-138
Certification of Horticultural Crops--A State Perspective....Pages 139-146
Plant Quarantine and International Shipment of Tissue Culture Plants....Pages 147-164
Summary of Panel Discussion on Plant Quarantines and International Shipment of Tissue Cultured Plants....Pages 165-166
Determining and Minimizing Production Costs....Pages 167-173
Determining Markets and Market Potential of Horticultural Crops....Pages 175-182
Propagation of Fruit, Nut, and Vegetable Crops — Overview....Pages 183-200
Small Fruit and Grape Tissue Culture from 1980 to 1985: Commercialization of the Technique....Pages 201-220
Temperate Fruits and Nuts....Pages 221-236
Tropical and Subtropical Fruits and Vegetables....Pages 237-251
Commercial in vitro Propagation and Plantation Crops....Pages 253-257
Use of Tissue Culture for Micropropagation of Vegetable Crops....Pages 259-270
Tissue Culture Techniques and Plant Introduction/Quarantine Procedures....Pages 271-282
Tissue Culture Propagation of Ornamental Crops: An Overview....Pages 283-292
Tissue Culture as a Plant Production System for Foliage Plants....Pages 293-299
Commercial Micropropagation of Florist Flower Crops....Pages 301-315
Woody Ornamentals, Shade Trees, and Conifers....Pages 317-331
Orchid Tissue Culture....Pages 333-342
Laboratory Design....Pages 343-349
Summary of Panel Discussion on Laboratory Design....Pages 351-364
Large Scale Tissue Culture Production for Horticultural Crops....Pages 365-366
....Pages 367-371
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