Ebook: Recent Advances in Biological Membrane Studies: Structure and Biogenesis Oxidation and Energetics

A NATO Advanced Study Institute on "New Developments and Methods in Membrane Research and Biological Energy Transduction" was held in order to consider some of the most recent developments in membrane research methodologies and results, with particular emphasis on studies of biological energy transduction. The partic­ ipants in the Institute dealt with three general areas of membrane study: membrane structure (with emphasis on lipid and protein components), membrane component assembly (with particular emphasis on mitochondria and chloroplasts), and the specialized functions of certain membrane systems. This last area included discussions of topics such as drug transformation, the role of membrane electron transport in the generation of oxygen radicals, the effect of oxygen radicals on cellular homeostasis and on the structure, organization and function of the acetylcholine receptor. Lectures and posters were concerned with two central questions: what is the function of membrane structure in energy transduction and how can energy trans­ duction be effectively measured and assessed? This text presents the content of the major lectures and important posters presented during the Institute's program. In issuing this book, the editor hopes to convey the proceedings of the Institute to a larger audi­ ence and to offer a comprehensive account of those developments in membrane research that were considered on the Island of Spetsai between August 16 and August 29, 1984. L. Packer Berkeley, California February 1985 v CONTENTS I. STRUCTURE AND BIOGENSIS Membrane Structure: Neutron Diffraction and Small Angle Scattering Studies •••••••••• 1 G.

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Content:
Front Matter....Pages N1-x
Membrane Structure: Neutron Diffraction and Small Angle Scattering Studies....Pages 1-15
The Nature of Proteins in Membranes....Pages 17-28
Phosphatidylcholine Transfer Protein: Model for Lipid-Protein Interactions and Probe for Membrane Research....Pages 29-43
Phosphatidylinositol Kinase, a Key Enzyme of Phosphatidylinositol Metabolism: Its Role in an Intracellular Second Messenger System....Pages 45-56
Electron Paramagnetic Resonance in Biology: Spin Trapping....Pages 57-64
Structure of Enveloped Viruses and Their Interaction with Cell Surfaces....Pages 65-74
Modification and Spin Labeling Studies of Membrane Bioenergetics....Pages 75-96
Spectroscopic Probes for Conformational Transients of Membrane Proteins....Pages 97-114
Determinations of Functional Heterogeneity in Proteoliposomes....Pages 115-130
Microenvironmental Properties and Conformational Changes in Glycogen Phosphorylase....Pages 131-147
Quantum Mechanical Model for Passive Transport through Bilayer Lipid Membranes....Pages 149-163
Origin of the Plastid Envelope Membranes....Pages 165-178
Pigment-Protein Complexes of Thylakoid Membranes: Assembly, Supramolecular Organization....Pages 179-203
Specific Properties of Higher Plant Mitochondria....Pages 205-236
Fast Membrane Transformation in a Flashing Endoplasmic Reticulum....Pages 237-257
Drug Biotransformation and Drug Toxicity Studied with Intact Mammalian Cells....Pages 259-284
Characterisation of the Nadph — Dependent Superoxide — Generating Oxidase of Phagocytic Leukocytes....Pages 285-300
Mechanisms of Membrane Lipid Peroxidation....Pages 301-317
Fluorescence Studies of Lipid Order in Proteoliposomes Containing Cytochrome P-490 and Its Reductase....Pages 319-344
The Role of Cations in the Photoinduced Electron Transport of Cyanobacteria....Pages 345-358
Halorhodopsin, the Light-Dependent Chloride Transport System of Halobacteria....Pages 359-368
The Determination of the Electrochemical Potential Difference of Protons in Bacterial Chromatophores....Pages 369-391
Light Induced Generation of a Proton Motive Force and Ca++-Transport in Membrane Vesicles of Streptococcus Cremoris Fused with Bacteriorhodopsin Proteoliposomes....Pages 393-407
Control of Mitochondrial Respiration....Pages 409-424
Molecular Study on Solute Carriers in Biomembranes, Exemplified by Two Well-Characterized Carriers from Mitochondria, the ADP/ATP Carrier and the Uncoupling Protein....Pages 425-437
The Mitochondrial F0–F1 Adenosine Triphosphatase: Isolation by Sepharose Hexylammonium Chromatography....Pages 439-462
The Unfolding of a Catalytic Mechanism for the Remarkable ATP Synthase....Pages 463-477
Membrane-Oscillator Hypothesis of Metabolic Control in Photoperiodic Time Measurement and the Temporal Organization of Development and Behaviour in Plants....Pages 479-497
Back Matter....Pages 499-510
....Pages 511-524