Ebook: Spectroscopy of Biological Molecules: Theory and Applications — Chemistry, Physics, Biology, and Medicine

This volume contains the proceedings of the NATO-Advanced Study Institute on the "Spectroscopy of Biological Molecules", which took place on July 4-15, 1983 in Acquafredda di Maratea, Italy. The institute concentrated on three main subiects: the structure and dymanics of DNA, proteins, and visual and plant pigments. Its timeliness has been linked to rapid advances in certain spectroscopic techniques which yielded a consider­ able amount of new information on the structure and inter­ actions of biologically important molecules. Among these techniques Fourier transform infrared, resonance and surface enhanced 'Raman spectroscopies, Raman microscopy and micro­ probing, time resolved techniques, two photon and ultrafast electronic, and C-13, N-15 and P-31 NMR spectroscopies and kinetic and static IR difference spectroscopy receiced a great deal of attention at the Institute. In addition, an entirely new technique, near-millimeter-wave spectroscopy has been presented and discussed. Two introductory quantum chemical lectures, one on the structure of water in DNA, and another pn the energy bands in DNA and proteins set the stage for the experimentally oriented lectures that followed. Fundamental knowledge on hydrogen bonding was the topic of two other lectures. Panel discussions were held on the structure and confor­ mations of DNA, metal-DNA adducts and proteins and on visual pigments. Many scientists who normally attend different conferences and never meet, met at Aquafredda di Maratea. We feel, that at the end of our Institute a synthetic vi~w emerged on the powerful spectroscopic and theoretical methods which are now available for the study of biological molecules.

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Content:
Front Matter....Pages i-x
Energy Bands in DNA....Pages 1-13
H-Bond Charge-Relay Chains in Multi-Heme Cytochromes and Other Biomolecules....Pages 15-37
Hydrogen Bonding — Theoretical and Spectroscopic Aspects....Pages 39-60
Hydrogen Bonds in Biological Structure and Mechanism....Pages 61-85
Raman Studies on Dinucleoside Monophosphates....Pages 87-89
Molecular Interaction between Nucleic Acids and Alkylating Agents by Raman Spectroscopy....Pages 91-111
Spectroscopic Properties of Metal-Nucleotide and Metal-Nucleic Acid Interactions....Pages 113-136
Conformation and Dynamics of Nucleic Acids and Proteins from Laser Raman Spectroscopy....Pages 137-152
Structural Transitions in DNA (A,B,Z) Studied by IR Spectroscopy....Pages 153-170
Raman Spectroscopy of Biomaterials Acting as Bone Prosthesis....Pages 171-189
Teaching the New NMR: A Computer-Aided Introduction to the Density Matrix Formalism of Multipulse Sequences....Pages 191-211
High Resolution 1H NMR Studies of Mononucleotides with Metals....Pages 213-256
Resonance Raman Determination of Retinal Chromophore Structure in Bacteriorhodopsin....Pages 257-290
Structural and Kinetic Studies of Bacteriorhodopsin by Resonance Raman Spectroscopy....Pages 291-301
Static and Time-Resolved Infrared Difference Spectroscopy Applied to Rhodopsin and Bacteriorhodopsin....Pages 303-328
Intermediate States in Vision....Pages 329-346
Correlation of Cellular and Molecular Changes in Visual Photoreceptors by Light Scattering Relaxation Photometry....Pages 347-372
Preparation of 2H and 13C Labeled Retinals....Pages 373-384
An Introduction to Two-Photon Spectroscopy....Pages 385-407
Two-Photon Spectroscopy of Biological Molecules....Pages 409-445
Molecular Structure of the Gramicidin Transmembrane Channel: Utilization of Carbon-13 Nuclear Magnetic Resonance, Ultraviolet Absorption, Circular Dichroism and Infrared Spectroscopies....Pages 447-455
Ionic Mechanisms and Selectivity of the Gramicidin Transmembrane Channel: Cation Nuclear Magnetic Resonance, Dielectric Relaxation, Carbon-13 Nuclear Magnetic Resonance, and Rate Theory Calculation of Single Channel Currents....Pages 457-471
In Situ Monitoring of Membrane-Bound Reactions by Kinetic Light-Scattering....Pages 473-486
Fourier Transform Infrared Spectroscopy as a Probe of Biomembrane Structure....Pages 487-510
Investigations on Biologically Important Hydrogen Bonds....Pages 511-538
A Review of the Fourier Transform Near Infrared Spectrometer for the Determination of Non-Metals in Organic Compounds by Atomic Emission from an Atmospheric Pressure Argon Inductively Coupled Plasma....Pages 539-545
Raman Microprobe and Microscope. Time Resolved Raman Techniques....Pages 547-561
Far-Infrared Spectroscopy of Biomolecules....Pages 563-570
Picosecond Relaxations in Haemoglobin, Lysozyme, and Poly-L-Alanine Observed by MM-Wave Spectroscopy....Pages 571-586
Back Matter....Pages 587-607
....Pages 609-619