Ebook: Gene Regulation by Steroid Hormones II

Receptors and Gene Expression It is now more than three years since the last Meadow Brook Conference on Hormones, and a great deal has happened in the meantime. We have become comfortable with the totally unanticipated fact that the coding sequences of genes are in discontinuous arrangements and that the RNA transcribed from them must be extensively processed to form messenger RNA. We have also learned about the strategy of "mixing and matching" of genetic segments so that a small amount of DNA can go a long way in producing a huge variety of different proteins, as in the immunoglobulin system. The explosive effort directed toward DNA sequence analysis has led us to the conclusion that there are signals within the DNA that specify sites of transcription initiation and possibly sites for interacting with regulatory molecules such as hor­ mones and their receptors. The current intense interest in the structure of chromatin beyond the nucleosome-that is, the superstructural characteris­ tics of the genetic material-is finally yielding meaningful results that give promise for understanding the regulation of gene activity. ROBERT F. GOLDBERGER Preface Research on the molecular mechanism of steroid hormone action continues at an extraordinary pace and a great deal of progress has been made. Steroid hormones have been localized on target genes providing the long awaited evidence for the concept of a direct effect of the steroid-receptor complex on gene regulation. Purified steroid receptors have been dissected to identify different functional domains.

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Content:
Front Matter....Pages I-XIII
Gene Structure and Evolution....Pages 1-16
Effects of Steroid Hormones on Chicken Oviduct Chromatin....Pages 17-59
Estrogen Receptor Regulation of Vitellogenin Gene Transcription and Chromatin Structure....Pages 61-78
Ecdysterone, Ecdysterone Receptor, and Chromosome Puffs....Pages 79-91
Ecdysteroid Effects on the Cell Cycle of Drosophila melanogaster Cells....Pages 93-99
Structure and Function of Growth Hormone—A Target for Glucocorticoid Action....Pages 101-103
Mechanism of Extinction of Growth Hormone and Prolactin Genes in Somatic Cell Hybrids....Pages 105-117
Studies of the Mechanism of Glucocorticoid Hormone Action....Pages 119-133
Corticosteroid Binder IB, A Potential Second Glucocorticoid Receptor....Pages 135-149
Functional Analysis of the Glucocorticoid Receptor by Limited Proteolysis....Pages 151-180
Identification of the DNA-Binding Domain of the Chicken Progesterone Receptor A Subunit....Pages 181-190
Immunochemical Studies of Estrogen Receptor....Pages 191-200
A Comparison of Central and Peripheral Estrogen Targets....Pages 201-217
Biological Role of Type II Estrogen-Binding Sites and Steroid Hormone Action....Pages 219-238
Hormone Receptors and the Nuclear Matrix....Pages 239-266
Alpha-Protein: A Marker for Androgen Action in the Rat Ventral Prostate....Pages 267-275
How Changes in Cytosol and Nuclear Androgen Receptors Relate to the Testosterone Responses: Studies with New Exchange Assays....Pages 277-298
Modulation of Androgen Action in Rat Liver by Thyroid and Peptide Hormones....Pages 299-310
A New Class of 5?-Reductase Inhibitors....Pages 311-334
Comments on Steroid Hormone Action....Pages 335-341
Back Matter....Pages 343-353

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Content:
Front Matter....Pages I-XIII
Gene Structure and Evolution....Pages 1-16
Effects of Steroid Hormones on Chicken Oviduct Chromatin....Pages 17-59
Estrogen Receptor Regulation of Vitellogenin Gene Transcription and Chromatin Structure....Pages 61-78
Ecdysterone, Ecdysterone Receptor, and Chromosome Puffs....Pages 79-91
Ecdysteroid Effects on the Cell Cycle of Drosophila melanogaster Cells....Pages 93-99
Structure and Function of Growth Hormone—A Target for Glucocorticoid Action....Pages 101-103
Mechanism of Extinction of Growth Hormone and Prolactin Genes in Somatic Cell Hybrids....Pages 105-117
Studies of the Mechanism of Glucocorticoid Hormone Action....Pages 119-133
Corticosteroid Binder IB, A Potential Second Glucocorticoid Receptor....Pages 135-149
Functional Analysis of the Glucocorticoid Receptor by Limited Proteolysis....Pages 151-180
Identification of the DNA-Binding Domain of the Chicken Progesterone Receptor A Subunit....Pages 181-190
Immunochemical Studies of Estrogen Receptor....Pages 191-200
A Comparison of Central and Peripheral Estrogen Targets....Pages 201-217
Biological Role of Type II Estrogen-Binding Sites and Steroid Hormone Action....Pages 219-238
Hormone Receptors and the Nuclear Matrix....Pages 239-266
Alpha-Protein: A Marker for Androgen Action in the Rat Ventral Prostate....Pages 267-275
How Changes in Cytosol and Nuclear Androgen Receptors Relate to the Testosterone Responses: Studies with New Exchange Assays....Pages 277-298
Modulation of Androgen Action in Rat Liver by Thyroid and Peptide Hormones....Pages 299-310
A New Class of 5?-Reductase Inhibitors....Pages 311-334
Comments on Steroid Hormone Action....Pages 335-341
Back Matter....Pages 343-353
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