Ebook: Protein Sequence Determination: A Sourcebook of Methods and Techniques
- Tags: Life Sciences general, Biomedicine general
- Series: Molecular Biology Biochemistry and Biophysics 8
- Year: 1975
- Publisher: Springer-Verlag Berlin Heidelberg
- Edition: 2
- Language: English
- pdf
In the few years since the first edition of this book made its appearance, under takings in the area of protein sequence determination have increased at an almost logarithmic pace. The logic governing such efforts has, predictably, retreated from a simple sense of curiosity in achieving something that had not previously been done, to be replaced by an absolute requirement for sequence information as the best means for understanding heretofore unattainable aspects of chemical, and structural and functional mechanistic phenomena. Witness, for example, recent volumes of the Annual Review of Biochemistry and other review journals, which treat the genetic control of mitochondrial proteins, mechanisms of enzyme action, the immunochemistry of collagen, and the role of lysosomal enzymes in the lipid storage diseases - all on the basis of known protein sequence information. One must note, too, the appearance of related works in the area of nucleic acid sequencing. The technologic advances in chemical sequencing procedures have proliferated at a remarkable rate. It is in the area of instrumentation, however, that the greatest advances have taken place. As a consequence of this, each chapter (with one single exception) in this book has been extensively revised and updated. Entire chapters have been replaced in several cases, without minimizing the value of their original content. In addition, a second volume will appear soon, dealing with subjects not covered in the present book.
Content:
Front Matter....Pages I-XVIII
General Considerations....Pages 1-4
Physical Characterization of the Protein Molecule....Pages 5-29
End Group Determination....Pages 30-103
Improved Tritium-Labeling for Quantitative C-Terminal Analysis....Pages 104-113
Fragmentation of Proteins for Sequence Studies and Separation of Peptide Mixtures....Pages 114-161
Identification of Specific Amino Acid Residues....Pages 162-203
Amino Acid Composition by Column Chromatography....Pages 204-231
Sequence Determination....Pages 232-279
Analysis of Amino Acid Phenylthiohydantoins by Gas Chromatography and High Performance Liquid Chromatography....Pages 280-297
Reconstruction of the Primary Sequence of a Protein from Peptides of Known Sequence....Pages 298-321
Peptide Synthesis....Pages 322-356
Back Matter....Pages 357-396
Content:
Front Matter....Pages I-XVIII
General Considerations....Pages 1-4
Physical Characterization of the Protein Molecule....Pages 5-29
End Group Determination....Pages 30-103
Improved Tritium-Labeling for Quantitative C-Terminal Analysis....Pages 104-113
Fragmentation of Proteins for Sequence Studies and Separation of Peptide Mixtures....Pages 114-161
Identification of Specific Amino Acid Residues....Pages 162-203
Amino Acid Composition by Column Chromatography....Pages 204-231
Sequence Determination....Pages 232-279
Analysis of Amino Acid Phenylthiohydantoins by Gas Chromatography and High Performance Liquid Chromatography....Pages 280-297
Reconstruction of the Primary Sequence of a Protein from Peptides of Known Sequence....Pages 298-321
Peptide Synthesis....Pages 322-356
Back Matter....Pages 357-396
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