Ebook: Cytochromes P-450 and b5 : Structure, Function, and Interaction

P-450 has in common with our nation that it can call Phila­ delphia its hometown. Yet there are differences, too. The U. S. A. was born and named in this city. P-450 was first recog­ nized here -- an odd CO-combining pigment without family or func­ tion. Japanese workers identified it as an unusual member of the cytochrome family. Finally, in Philadelphia, its biological func­ tion was established and its growth to a global power initiated. Since discovery of its function as terminal oxidase of the 21-steroid hydroxylase system of adrenocortical microsomes, P-450 has proved to play the same role in a wide variety of other mixed function oxidase systems involved in biosynthesis and catabolism of specific cell or body components as well as in the metabolism of foreign substances entering organisms. P-450-like oxygenating enzymes appear to be fundamental cellular constituents in most forms of aerobic organisms. Activation of molecular oxygen and incorporation of one of its atoms into organic compounds as cata­ lyzed by P-450 enzymes are reactions of vital importance not only for biosynthesis and degradation of steroid hormones necessary for sustaining life, but also for metabolic activation or inactivation of foreign agents such as drugs, food preservatives and additives, insecticides and carcinogens. Moreover, P-450 linked enzyme sys­ tems can either be induced or suppressed by these agents with significant biological consequences.

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Content:
Front Matter....Pages i-xiii
Partial Purification and Separation of Multiple Forms of Cytochrome P-450 and Cytochrome P-448 from Rat Liver Microsomes....Pages 1-24
Biochemical Characterization of Highly Purified Cytochrome P-450 and Other Components of the Mixed Function Oxidase System of Liver Microsomal Membranes....Pages 25-46
Immunochemical and Compositional Comparison of Cytochrome P-450cam of Pseudomonas Putida and P-450LM of Phenobarbital-Induced Rabbit Liver Microsomes....Pages 47-53
Immunochemical and Functional Similarities and Differences among Iron-Sulfur Proteins Involved in Mammalian Steroidogenesis....Pages 55-71
General Discussion....Pages 73-80
Optical and Electron Paramagnetic Resonance Studies of Partially Purified Rabbit Liver Cytochrome P-450....Pages 175-187
Studies on the Spin State of 3-Methylcholanthrene Induced Cytochrome P-450 from Rat Liver....Pages 189-202
An Analysis of the Optical Titrations of the 430 and 455 NM Chromophores of Ethyl Isocyanide Complexes of Mammalian Hepatic Cytochrome P-450....Pages 203-212
Implication of Ligand Modified Spectra of Cytochrome P-450 Associated with Pregnenolone Synthesis in Mitochondria from Corpus Luteum....Pages 213-227
General Discussion....Pages 229-237
Comparison of the Induction Course, Biophysical Chemical Interactions and Photochemical Action Spectra of Phenobarbital and 3-Methylcholanthrene Induced Hepatic Microsomal P-450....Pages 81-102
Cytochrome P-450 in the Activation and Inactivation of Carcinogens....Pages 103-115
Effect of Cyclic Amp on the Phenobarbital Induced Increase in Cytochrome P-450 and Hypertrophy of the Endoplasmic Reticulum of the Rat Liver....Pages 117-126
Evidence for the Activation of 3-Methylcholanthrene as a Carcinogen in vivo and as a Mutagen in vitro by P1-450 from Inbred Strains of Mice....Pages 127-149
Increased Translation as a Result of Elevated Initiation Factor Activity after Administration of 3-Methylcholanthrene....Pages 151-164
Discussion....Pages 165-173
Studies on the Interaction of Water with Microsomal Cytochrome P-450....Pages 239-249
Drug Metabolism in Isolated Rat Liver Cells....Pages 251-269
Role of Phospholipids in Adrenocortical Microsomal Hydroxylation Reactions: Activation of Lipid-Depleted Microsomal Preparations by Non-Ionic Detergents....Pages 271-286
On the Structure of Putidaredoxin and Cytochrome P-450cam and their Mode of Interaction....Pages 287-309
Optical and Electron Paramagnetic Resonance Studies of Partially Purified Rabbit Liver Cytochrome P-450....Pages 175-187
Studies on the Spin State of 3-Methylcholanthrene Induced Cytochrome P-450 from Rat Liver....Pages 189-202
An Analysis of the Optical Titrations of the 430 and 455 NM Chromophores of Ethyl Isocyanide Complexes of Mammalian Hepatic Cytochrome P-450....Pages 203-212
Implication of Ligand Modified Spectra of Cytochrome P-450 Associated with Pregnenolone Synthesis in Mitochondria from Corpus Luteum....Pages 213-227
General Discussion....Pages 229-237
Studies on the Interaction of Water with Microsomal Cytochrome P-450....Pages 239-249
Drug Metabolism in Isolated Rat Liver Cells....Pages 251-269
Role of Phospholipids in Adrenocortical Microsomal Hydroxylation Reactions: Activation of Lipid-Depleted Microsomal Preparations by Non-Ionic Detergents....Pages 271-286
On the Structure of Putidaredoxin and Cytochrome P-450cam and their Mode of Interaction....Pages 287-309
General Discussion....Pages 311-324
Relationship Between Microsomal Hydroxylase and Glucuronyltransferase....Pages 325-333
A Possible Role of Copper in the Regulation of Heme Biosynthesis Through Ferrochelatase....Pages 335-341
Mixed Function Oxidation and Intermediary Metabolism: Metabolic Interdependencies in the Liver....Pages 343-354
Dissociation of Microsomal Ethanol Oxidation from Cytochrome P-450 Catalyzed Drug Metabolism....Pages 355-367
General Discussion....Pages 369-382
Interaction Between Microsomal Electron Transfer Pathways....Pages 383-386
Role of Cytochrome b5 in the NADH Synergism of NADPH-Dependent Reactions of the Cytochrome P-450 Monooxygenase System of Hepatic Microsomes....Pages 387-404
The Role of Cytochrome b5 in Cytochrome P-450 Enzymes....Pages 405-434
Role of Cytochrome b5 in NADPH- and NADH-Dependent Hydroxylation by the Reconstituted Cytochrome P-450- or P-448-Containing System....Pages 435-445
The Role of Cytochrome b5 in Mixed Function Oxidations: Effect of Microsomal Binding of the Hemoprotein on Hepatic N-Demethylations....Pages 447-466
Comparison of Methods to Study Enzyme Induction in Man....Pages 467-483
General Discussion....Pages 485-502
Back Matter....Pages 503-510
....Pages 511-554