Ebook: Modern Methods of Plant Analysis / Moderne Methoden der Pflanzenanalyse
- Tags: Life Sciences general, Biomedicine general
- Series: Modern Methods of Plant Analysis / Moderne Methoden der Pflanzenanalyse 5
- Year: 1962
- Publisher: Springer-Verlag Berlin Heidelberg
- Edition: 1
- Language: English-German-French
- pdf
123 phase and hence have no direct bearing on the retention time of solutes. However in gas-solid chromatography, a considerable quantity of the mobile phase may be adsorbed on the surface of the stationary adsorbent which diminishes the column's effective length and ability to retain solutes. In this respect helium has been found to be preferable to most other gases (GREENE and Roy, 1957) because it is adsorbed to the least extent. 3. Packed columns offer a considerable resistance to flow, which may create a pressure differential between inlet and outlet of sufficient magnitude to cause an unfavorable flow rate through a significant length of the column. A reduced inlet/outlet pressure ratio can be obtained by using light molecular weight gases toward which the column packing shows the greatest permeability. The flow rate of the mobile phase is normally adjusted by altering the column inlet pressure, for which purpose commercial pressure regulators of sufficient accuracy are available. Quantitative measurements of the flow rate can be made by a number of methods, including rotameters, orifice meters, soapfilm flow meters and displacement of water. The former two methods are the most con venient but the least accurate; moreover they create a back pressure and are temperature dependent whereas although the moving soap bubble is cumbersome to employ and unusable for continuous readings, it is preferred when the highest accuracy is required.
Content:
Front Matter....Pages II-XXVII
Emission and Atomic Absorption Spectrochemical Methods....Pages 1-25
Mass Spectrometric Methods....Pages 26-50
Plant Spectra: Absorption and Action....Pages 51-72
Gefriertrocknung....Pages 73-96
Vapour Phase Chromatography....Pages 97-158
Ion-Exchange Chromatography....Pages 159-204
Molecular Sieving other than Dialysis....Pages 205-213
Dunnschicht-Chromatographie....Pages 214-229
Paper Chromatography on a Preparative Scale....Pages 230-249
Determination of Size, Shape and Homogeneity of Macromolecules in Solution....Pages 250-324
Optical Rotatory Dispersion. Its Application to Protein Conformation....Pages 325-335
Diffuse Rontgenkleinwinkelstreuung....Pages 336-358
Methodes Calorimetriques pour l’;Analyse des Vegetaux....Pages 359-367
Surface Factors Affecting the Penetration of Compounds into Plants....Pages 368-382
Tissue and Single Cell Cultures of Higher Plants as a Basic Experimental Method....Pages 383-421
Immunological Methods....Pages 422-463
Polarography and Tensammetry....Pages 464-493
Fallout Contamination in Plants....Pages 494-509
Back Matter....Pages 510-536
Content:
Front Matter....Pages II-XXVII
Emission and Atomic Absorption Spectrochemical Methods....Pages 1-25
Mass Spectrometric Methods....Pages 26-50
Plant Spectra: Absorption and Action....Pages 51-72
Gefriertrocknung....Pages 73-96
Vapour Phase Chromatography....Pages 97-158
Ion-Exchange Chromatography....Pages 159-204
Molecular Sieving other than Dialysis....Pages 205-213
Dunnschicht-Chromatographie....Pages 214-229
Paper Chromatography on a Preparative Scale....Pages 230-249
Determination of Size, Shape and Homogeneity of Macromolecules in Solution....Pages 250-324
Optical Rotatory Dispersion. Its Application to Protein Conformation....Pages 325-335
Diffuse Rontgenkleinwinkelstreuung....Pages 336-358
Methodes Calorimetriques pour l’;Analyse des Vegetaux....Pages 359-367
Surface Factors Affecting the Penetration of Compounds into Plants....Pages 368-382
Tissue and Single Cell Cultures of Higher Plants as a Basic Experimental Method....Pages 383-421
Immunological Methods....Pages 422-463
Polarography and Tensammetry....Pages 464-493
Fallout Contamination in Plants....Pages 494-509
Back Matter....Pages 510-536
....