Ebook: Biophysics of RNA Folding
- Tags: Molecular Medicine, Biophysics and Biological Physics, Biochemistry general, Nucleic Acid Chemistry, Single Molecule Studies Molecular Motors
- Series: Biophysics for the Life Sciences 3
- Year: 2013
- Publisher: Springer-Verlag New York
- Edition: 1
- Language: English
- pdf
This volume, written by experts in the field, discusses the current understanding of the biophysical principles that govern RNA folding, with featured RNAs including the ribosomal RNAs, viral RNAs, and self-splicing introns. In addition to the fundamental features of RNA folding, the central experimental and computational approaches in the field are presented with an emphasis on their individual strengths and limitations, and how they can be combined to be more powerful than any method alone; these approaches include NMR, single molecule fluorescence, site-directed spin labeling, structure mapping, comparative sequence analysis, graph theory, course-grained 3D modeling, and more. This volume will be of interest to professional researchers and advanced students entering the field of RNA folding.
Structured RNAs are everywhere, functioning throughout gene expression with key roles ranging from catalysis to regulation. New functional RNAs are being discovered all the time; in fact, it is now clear that a much greater fraction of eukaryotic genomes is devoted to coding for RNA than protein. Many of these RNAs must traverse complex energy landscapes to find their functional three-dimensional structures. Along the way, they may encounter native and non-native folding intermediates, chaperone proteins, and assemble with partner proteins.
This volume, written by experts in the field, discusses the current understanding of the biophysical principles that govern RNA folding, with featured RNAs including the ribosomal RNAs, viral RNAs, and self-splicing introns. In addition to the fundamental features of RNA folding, the central experimental and computational approaches in the field are presented with an emphasis on their individual strengths and limitations, and how they can be combined to be more powerful than any method alone; these approaches include NMR, single molecule fluorescence, site-directed spin labeling, structure mapping, comparative sequence analysis, graph theory, course-grained 3D modeling, and more. This volume will be of interest to professional researchers and advanced students entering the field of RNA folding.
Structured RNAs are everywhere, functioning throughout gene expression with key roles ranging from catalysis to regulation. New functional RNAs are being discovered all the time; in fact, it is now clear that a much greater fraction of eukaryotic genomes is devoted to coding for RNA than protein. Many of these RNAs must traverse complex energy landscapes to find their functional three-dimensional structures. Along the way, they may encounter native and non-native folding intermediates, chaperone proteins, and assemble with partner proteins.
This volume, written by experts in the field, discusses the current understanding of the biophysical principles that govern RNA folding, with featured RNAs including the ribosomal RNAs, viral RNAs, and self-splicing introns. In addition to the fundamental features of RNA folding, the central experimental and computational approaches in the field are presented with an emphasis on their individual strengths and limitations, and how they can be combined to be more powerful than any method alone; these approaches include NMR, single molecule fluorescence, site-directed spin labeling, structure mapping, comparative sequence analysis, graph theory, course-grained 3D modeling, and more. This volume will be of interest to professional researchers and advanced students entering the field of RNA folding.
Content:
Front Matter....Pages i-vi
Introduction and Overview....Pages 1-10
Comparative Analysis of the Higher-Order Structure of RNA....Pages 11-22
Graph Applications to RNA Structure and Function....Pages 23-51
Prediction and Coarse-Grained Modeling of RNA Structures....Pages 53-68
Studying RNA Folding Using Site-Directed Spin Labeling....Pages 69-87
The RNA Recognition Motif and Messenger RNA....Pages 89-116
Memory Effects in RNA Folding Dynamics Revealed by Single-Molecule Fluorescence....Pages 117-133
An Integrated Picture of HDV Ribozyme Catalysis....Pages 135-167
Combining Biochemical and Structural Information to Model RNA-Protein Complex Assembly....Pages 169-186
Following RNA Folding From Local and Global Perspectives....Pages 187-203
The Roles of Chaperones in RNA Folding....Pages 205-230
Back Matter....Pages 231-236
Structured RNAs are everywhere, functioning throughout gene expression with key roles ranging from catalysis to regulation. New functional RNAs are being discovered all the time; in fact, it is now clear that a much greater fraction of eukaryotic genomes is devoted to coding for RNA than protein. Many of these RNAs must traverse complex energy landscapes to find their functional three-dimensional structures. Along the way, they may encounter native and non-native folding intermediates, chaperone proteins, and assemble with partner proteins.
This volume, written by experts in the field, discusses the current understanding of the biophysical principles that govern RNA folding, with featured RNAs including the ribosomal RNAs, viral RNAs, and self-splicing introns. In addition to the fundamental features of RNA folding, the central experimental and computational approaches in the field are presented with an emphasis on their individual strengths and limitations, and how they can be combined to be more powerful than any method alone; these approaches include NMR, single molecule fluorescence, site-directed spin labeling, structure mapping, comparative sequence analysis, graph theory, course-grained 3D modeling, and more. This volume will be of interest to professional researchers and advanced students entering the field of RNA folding.
Content:
Front Matter....Pages i-vi
Introduction and Overview....Pages 1-10
Comparative Analysis of the Higher-Order Structure of RNA....Pages 11-22
Graph Applications to RNA Structure and Function....Pages 23-51
Prediction and Coarse-Grained Modeling of RNA Structures....Pages 53-68
Studying RNA Folding Using Site-Directed Spin Labeling....Pages 69-87
The RNA Recognition Motif and Messenger RNA....Pages 89-116
Memory Effects in RNA Folding Dynamics Revealed by Single-Molecule Fluorescence....Pages 117-133
An Integrated Picture of HDV Ribozyme Catalysis....Pages 135-167
Combining Biochemical and Structural Information to Model RNA-Protein Complex Assembly....Pages 169-186
Following RNA Folding From Local and Global Perspectives....Pages 187-203
The Roles of Chaperones in RNA Folding....Pages 205-230
Back Matter....Pages 231-236
....