Ebook: Dynamics and the Problem of Recognition in Biological Macromolecules

From within complex structures of organisms and cells down to the molecular level, biological processes all involve movement. Muscular fibers slide on each other to activate the muscle, as polymerases do along nucleic acids for replicating and transcribing the genetic material. Cells move and organize themselves into organs by recognizing each other through macromolecular surface-specific interactions. These recognition processes involve the mu­ tual adaptation of structures that rely on their flexibility. All sorts of conformational changes occur in proteins involved in through-membrane signal transmission, showing another aspect of the flexibility of these macromolecules. The movement and flexibility are inscribed in the polymeric nature of essential biological macromolecules such as proteins and nucleic acids. For instance, the well-defined structures formed by the long protein chain are held together by weak noncovalent interac­ tions that design a complex potential well in which the protein floats, permanently fluctuating between several micro- or macroconformations in a wide range of frequencies and ampli­ tudes. The inherent mobility of biomolecular edifices may be crucial to the adaptation of their structures to particular functions. Progress in methods for investigating macromolecular structures and dynamics make this hypothesis not only attractive but more and more testable.

---

Protein Structures: The Cooperative Substructure of Protein Molecules; Y. Bai, S.W. Englander.Protein Folding: Approaches to the Determination of More Accurate Crossrelaxation Rates and the Effects of Improved Distance Constraints on Protein Solution Structures; G.C.Hoogstraten, J.L. Markley.Protein Interactions: How Conventional Antigens and Superantigens Interact with the Human MHC Class II Molecule HLADR1; T. Jardetzky.Nucleic Acids and Nucleic Acid-ProteinInteractions: Stimulating the Dynamics of the DNA Double Helix in Solution; M. Hirshberg, M. Levitt.Membranes: Applications of Multidimensional Solidstate NMR Spectroscopy to Membrane Proteins; A.Ramamoorthy, et al.Carbohydrate Structure: Conformation, Mobility, and Function of the N-linked Glycan in the Adhesion Domain of Human CD2; G. Wagner, et al.Abstracts from Course: Protein StructuresAbstracts: Solution Structure of the ETS-domain from Murine Ets1: A Winged-helix-turn-helix Motif; L.W. Donaldson.Protein InteractionsAbstracts: Designing Mutant Hemoglobins; M. Madrid, et al.NucleicAcids and Nucleic Acid-Protein Interactions Abstracts: A Study on the Dynamics of a DNA Binding Protein; L.M. Horstink, et al. Poster Abstracts. 55 additional articles. Index.

---

Protein Structures: The Cooperative Substructure of Protein Molecules; Y. Bai, S.W. Englander.Protein Folding: Approaches to the Determination of More Accurate Crossrelaxation Rates and the Effects of Improved Distance Constraints on Protein Solution Structures; G.C.Hoogstraten, J.L. Markley.Protein Interactions: How Conventional Antigens and Superantigens Interact with the Human MHC Class II Molecule HLADR1; T. Jardetzky.Nucleic Acids and Nucleic Acid-ProteinInteractions: Stimulating the Dynamics of the DNA Double Helix in Solution; M. Hirshberg, M. Levitt.Membranes: Applications of Multidimensional Solidstate NMR Spectroscopy to Membrane Proteins; A.Ramamoorthy, et al.Carbohydrate Structure: Conformation, Mobility, and Function of the N-linked Glycan in the Adhesion Domain of Human CD2; G. Wagner, et al.Abstracts from Course: Protein StructuresAbstracts: Solution Structure of the ETS-domain from Murine Ets1: A Winged-helix-turn-helix Motif; L.W. Donaldson.Protein InteractionsAbstracts: Designing Mutant Hemoglobins; M. Madrid, et al.NucleicAcids and Nucleic Acid-Protein Interactions Abstracts: A Study on the Dynamics of a DNA Binding Protein; L.M. Horstink, et al. Poster Abstracts. 55 additional articles. Index.
Content:
Front Matter....Pages i-viii
The Cooperative Substructure of Protein Molecules....Pages 1-7
NMR Structures of Serine Proteinase Inhibitors LDTI and RBI, and Comparison With X-ray Structures....Pages 9-27
Solution Structure of the Long Neurotoxin LSIII with Possible Implications for Binding to the Acetylcholine Receptor....Pages 29-35
NMR Studies of Enzyme-Substrate and Protein-Protein Interactions....Pages 37-47
The Direct Determination of Protein Structure from Multidimensional NMR Spectra without Assignment....Pages 49-55
Accuracy of Nuclear Magnetic Resonance Derived Molecular Structures....Pages 57-63
What Limits Protein Folding....Pages 65-71
Approaches to the Determination of More Accurate Cross-Relaxation Rates and the Effects of Improved Distance Constraints on Protein Solution Structures....Pages 73-111
Protein Dynamics....Pages 113-126
Insights into Protein Dynamics by NMR Techniques....Pages 127-138
Heteronuclear Relaxation and the Experimental Determination of the Spectral Density Function....Pages 139-162
How Conventional Antigens and Superantigens Interact with the Human MHC Class II Molecule HLA-DR1....Pages 163-172
Simulating the Dynamics of the DNA Double Helix in Solution....Pages 173-191
Developments in NMR Structure Determination of Nucleic Acids....Pages 193-207
Solution Dynamics of the TRP-Repressor Studied by NMR Spectroscopy....Pages 209-222
A Refined NMR Solution Structure of the POU-Specific Domain of the Human OCT-1 Protein....Pages 223-236
Applications of Multidimensional Solid-State NMR Spectroscopy to Membrane Proteins....Pages 237-255
Conformation, Mobility, and Function of the N-Linked Glycan in the Adhesion Domain of Human CD2....Pages 257-266
Abstracts....Pages 267-299
Back Matter....Pages 301-311

---

Protein Structures: The Cooperative Substructure of Protein Molecules; Y. Bai, S.W. Englander.Protein Folding: Approaches to the Determination of More Accurate Crossrelaxation Rates and the Effects of Improved Distance Constraints on Protein Solution Structures; G.C.Hoogstraten, J.L. Markley.Protein Interactions: How Conventional Antigens and Superantigens Interact with the Human MHC Class II Molecule HLADR1; T. Jardetzky.Nucleic Acids and Nucleic Acid-ProteinInteractions: Stimulating the Dynamics of the DNA Double Helix in Solution; M. Hirshberg, M. Levitt.Membranes: Applications of Multidimensional Solidstate NMR Spectroscopy to Membrane Proteins; A.Ramamoorthy, et al.Carbohydrate Structure: Conformation, Mobility, and Function of the N-linked Glycan in the Adhesion Domain of Human CD2; G. Wagner, et al.Abstracts from Course: Protein StructuresAbstracts: Solution Structure of the ETS-domain from Murine Ets1: A Winged-helix-turn-helix Motif; L.W. Donaldson.Protein InteractionsAbstracts: Designing Mutant Hemoglobins; M. Madrid, et al.NucleicAcids and Nucleic Acid-Protein Interactions Abstracts: A Study on the Dynamics of a DNA Binding Protein; L.M. Horstink, et al. Poster Abstracts. 55 additional articles. Index.
Content:
Front Matter....Pages i-viii
The Cooperative Substructure of Protein Molecules....Pages 1-7
NMR Structures of Serine Proteinase Inhibitors LDTI and RBI, and Comparison With X-ray Structures....Pages 9-27
Solution Structure of the Long Neurotoxin LSIII with Possible Implications for Binding to the Acetylcholine Receptor....Pages 29-35
NMR Studies of Enzyme-Substrate and Protein-Protein Interactions....Pages 37-47
The Direct Determination of Protein Structure from Multidimensional NMR Spectra without Assignment....Pages 49-55
Accuracy of Nuclear Magnetic Resonance Derived Molecular Structures....Pages 57-63
What Limits Protein Folding....Pages 65-71
Approaches to the Determination of More Accurate Cross-Relaxation Rates and the Effects of Improved Distance Constraints on Protein Solution Structures....Pages 73-111
Protein Dynamics....Pages 113-126
Insights into Protein Dynamics by NMR Techniques....Pages 127-138
Heteronuclear Relaxation and the Experimental Determination of the Spectral Density Function....Pages 139-162
How Conventional Antigens and Superantigens Interact with the Human MHC Class II Molecule HLA-DR1....Pages 163-172
Simulating the Dynamics of the DNA Double Helix in Solution....Pages 173-191
Developments in NMR Structure Determination of Nucleic Acids....Pages 193-207
Solution Dynamics of the TRP-Repressor Studied by NMR Spectroscopy....Pages 209-222
A Refined NMR Solution Structure of the POU-Specific Domain of the Human OCT-1 Protein....Pages 223-236
Applications of Multidimensional Solid-State NMR Spectroscopy to Membrane Proteins....Pages 237-255
Conformation, Mobility, and Function of the N-Linked Glycan in the Adhesion Domain of Human CD2....Pages 257-266
Abstracts....Pages 267-299
Back Matter....Pages 301-311
....