Ebook: Single-Channel Recording

Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this Second Edition includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass pipettes; and patch clamping.

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Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this Second Edition includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass pipettes; and patch clamping.

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Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this Second Edition includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass pipettes; and patch clamping.
Content:
Front Matter....Pages i-xxvii
Front Matter....Pages 1-1
A Practical Guide to Patch Clamping....Pages 3-30
Tight-Seal Whole-Cell Recording....Pages 31-52
Guide to Data Acquisition and Analysis....Pages 53-91
Front Matter....Pages 93-93
Electronic Design of the Patch Clamp....Pages 95-127
Low-Noise Recording....Pages 129-145
Voltage Offsets in Patch-Clamp Experiments....Pages 147-153
Techniques for Membrane Capacitance Measurements....Pages 155-198
Patch-Pipette Recordings from the Soma, Dendrites, and Axon of Neurons in Brain Slices....Pages 199-211
Patch Clamp and Calcium Imaging in Brain Slices....Pages 213-229
Fast Application of Agonists to Isolated Membrane Patches....Pages 231-243
Electrochemical Detection of Secretion from Single Cells....Pages 245-275
Technical Approaches to Studying Specific Properties of Ion Channels in Plants....Pages 277-305
The Giant Membrane Patch....Pages 307-327
A Fast Pressure-Clamp Technique for Studying Mechanogated Channels....Pages 329-340
Electrophysiological Recordings from Xenopus Oocytes....Pages 341-356
Polymerase Chain Reaction Analysis of Ion Channel Expression in Single Neurons of Brain Slices....Pages 357-373
Force Microscopy on Membrane Patches....Pages 375-393
Front Matter....Pages 395-395
The Principles of the Stochastic Interpretation of Ion-Channel Mechanisms....Pages 397-482
Fitting and Statistical Analysis of Single-Channel Records....Pages 483-587
A Q-Matrix Cookbook....Pages 589-633
Front Matter....Pages 635-635
Geometric Parameters of Pipettes and Membrane Patches....Pages 637-650
Conformational Transitions of Ionic Channels....Pages 651-662
Back Matter....Pages 663-700

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Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this Second Edition includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass pipettes; and patch clamping.
Content:
Front Matter....Pages i-xxvii
Front Matter....Pages 1-1
A Practical Guide to Patch Clamping....Pages 3-30
Tight-Seal Whole-Cell Recording....Pages 31-52
Guide to Data Acquisition and Analysis....Pages 53-91
Front Matter....Pages 93-93
Electronic Design of the Patch Clamp....Pages 95-127
Low-Noise Recording....Pages 129-145
Voltage Offsets in Patch-Clamp Experiments....Pages 147-153
Techniques for Membrane Capacitance Measurements....Pages 155-198
Patch-Pipette Recordings from the Soma, Dendrites, and Axon of Neurons in Brain Slices....Pages 199-211
Patch Clamp and Calcium Imaging in Brain Slices....Pages 213-229
Fast Application of Agonists to Isolated Membrane Patches....Pages 231-243
Electrochemical Detection of Secretion from Single Cells....Pages 245-275
Technical Approaches to Studying Specific Properties of Ion Channels in Plants....Pages 277-305
The Giant Membrane Patch....Pages 307-327
A Fast Pressure-Clamp Technique for Studying Mechanogated Channels....Pages 329-340
Electrophysiological Recordings from Xenopus Oocytes....Pages 341-356
Polymerase Chain Reaction Analysis of Ion Channel Expression in Single Neurons of Brain Slices....Pages 357-373
Force Microscopy on Membrane Patches....Pages 375-393
Front Matter....Pages 395-395
The Principles of the Stochastic Interpretation of Ion-Channel Mechanisms....Pages 397-482
Fitting and Statistical Analysis of Single-Channel Records....Pages 483-587
A Q-Matrix Cookbook....Pages 589-633
Front Matter....Pages 635-635
Geometric Parameters of Pipettes and Membrane Patches....Pages 637-650
Conformational Transitions of Ionic Channels....Pages 651-662
Back Matter....Pages 663-700
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