Ebook: Squid as Experimental Animals

The predecessor to this book was A Guide to the Laboratory Use of the Squid Loligo pealei published by the Marine Biological Laboratory, Woods Hole, Massachusetts in 1974. The revision of this long out of date guide, with the approval of the Marine Biological Laboratory, is an attempt to introduce students and researchers to the cephalopods and particularly the squid as an object of biological research. Therefore, we have decided to expand on its original theme, which was to present important practical aspects for using the squid as experimental animals. There are twenty two chapters instead of the original eight. The material in the original eight chapters has been completely revised. Since more than one method can be used for accomplishing a given task, some duplication of methods was considered desirable in the various chapters. Thus, the methodology can be chosen which is best suited for each reader's requirements. Each subject also contains a mini-review which can serve as an introduction to the various topics. Thus, the volume is not just a laboratory manual, but can also be used as an introduction to squid biology. The book is intended for laboratory technicians, advanced undergraduate students, graduate students, researchers, and all others who want to learn the purpose, methods, and techniques of using squid as experimental animals. This is the reason why the name has been changed to its present title. Preceding the chapters is a list of many of the abbreviations, prefixes, and suffixes used in this volume.

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Content:
Front Matter....Pages i-xxxi
Front Matter....Pages 1-1
Evolution and Intelligence of the Cephalopods....Pages 3-9
Natural History and Collection....Pages 11-25
Maintenance, Rearing, and Culture of Teuthoid and Sepioid Squids....Pages 27-34
Front Matter....Pages 35-62
Squid Mating Behavior....Pages 63-63
Embryonic Development of the Squid....Pages 65-75
Front Matter....Pages 77-90
Electrophysiology and Biophysics of the Squid Giant Axon....Pages 91-91
Internal Dialysis in the Squid Giant Axon....Pages 93-132
The Cut-Open Axon Technique....Pages 133-152
Optical Measurements on Squid Axons....Pages 153-159
The Preparation of the Squid Giant Synapse for Electrophysiological Investigation....Pages 161-170
Front Matter....Pages 171-192
Tissue Culture of Squid Neurons, Glia, and Muscle Cells....Pages 193-193
Squid Optic Lobe Synaptosomes: Structure and Function of Isolated Synapses....Pages 195-212
The Cytoskeleton of the Squid Giant Axon....Pages 213-233
Studying Axoplasmic Transport by Video Microscopy and Using the Squid Giant Axon as a Model System....Pages 235-302
Lipid Metabolism In The Squid Nervous System....Pages 303-321
Front Matter....Pages 323-368
Structure and Function of the Squid Eye....Pages 369-369
Development of the Squid’s Visual System....Pages 371-397
The Statocysts of Squid....Pages 399-419
Front Matter....Pages 421-439
Gas Transport in the Blood....Pages 441-441
Front Matter....Pages 443-468
An Organophosphorus Detoxifying Enzyme Unique to Squid....Pages 441-441
Squid as Elite Athletes: Locomotory, Respiratory, and Circulatory Integration....Pages 469-480
Back Matter....Pages 481-503
....Pages 505-516