Ebook: Reaction Centers of Photosynthetic Bacteria: Feldafing-II-Meeting

Reaction Centers of Photosynthetic Bacteria is an updated record on the most recent insight into the struc- ture/function relationship of reaction centers from photosynthetic bacteria. It addresses in particular, interactions and dynamics which determine the ultra-high quantum yield of photoinduced charge separation in these energy-transforming molecular machines. Of particular interest is the still controversial issue of the primary charge separation mechanism as well as the effects of well-defined modifications, introduced either by mutagenic replacements in the protein matrix or by chemical exchange of reaction center pigments. Also described are the methods used for the characterization of interactions and dynamics important for electron transfer processes in the reaction center.

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Reaction Centers of Photosynthetic Bacteria is an updated record on the most recent insight into the struc- ture/function relationship of reaction centers from photosynthetic bacteria. It addresses in particular, interactions and dynamics which determine the ultra-high quantum yield of photoinduced charge separation in these energy-transforming molecular machines. Of particular interest is the still controversial issue of the primary charge separation mechanism as well as the effects of well-defined modifications, introduced either by mutagenic replacements in the protein matrix or by chemical exchange of reaction center pigments. Also described are the methods used for the characterization of interactions and dynamics important for electron transfer processes in the reaction center.

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Reaction Centers of Photosynthetic Bacteria is an updated record on the most recent insight into the struc- ture/function relationship of reaction centers from photosynthetic bacteria. It addresses in particular, interactions and dynamics which determine the ultra-high quantum yield of photoinduced charge separation in these energy-transforming molecular machines. Of particular interest is the still controversial issue of the primary charge separation mechanism as well as the effects of well-defined modifications, introduced either by mutagenic replacements in the protein matrix or by chemical exchange of reaction center pigments. Also described are the methods used for the characterization of interactions and dynamics important for electron transfer processes in the reaction center.
Content:
Front Matter....Pages I-XIV
Front Matter....Pages 1-1
Reaction Center from Wild Type Y Rhodobacter Sphaeroides: Primary Structure of the L and M Subunits; Carotenoid and Detergent Structures in the Three-Dimensional Crystals....Pages 3-9
Probing Reaction Center Asymmetry Using Low Temperature Absorption Spectroscopy of Rhodobacter sphaeroides Reaction Centers Containing Bacteriopheophytin Anions....Pages 11-18
Cytochrome Arrangement in Reaction Centers of Different Species of Photosynthetic Bacteria....Pages 19-29
Infrared Difference Spectroscopy of Electro-chemically Generated Redox States in Bacterial Reaction Centers....Pages 31-44
EPR and ENDOR Studies of the Oxidized Primary Donor in Single Crystals of Reaction Centers of Rhodobacter sphaeroides R-26....Pages 45-55
Triplet State ADMR of Bacterial Reaction Centers at Low Transition Frequencies....Pages 57-68
Investigation of Quinone Reduction in Rhodopseudomonas viridis by FTIR Difference Spectroscopy and X-Ray Diffraction Analysis....Pages 69-74
Steady-State and Time-Resolved FTIR Spectroscopy of Quinones in Bacterial Reaction Centers....Pages 75-85
Front Matter....Pages 87-98
Spectral Hole Burning Studies of Photosystem II Reaction Centers: Excited State Structure, Charge Separation and Energy Transfer Dynamics....Pages 99-99
Evidence for an Inhomogeneous Distribution of Reaction Centers on the Timescale of the Primary Electron Transfer Events....Pages 101-111
Analysis of Transient Absorption Data from Reaction Centers of Purple Bacteria....Pages 113-125
Picosecond Resonance Raman Spectroscopy of Rhodobacter sphaeroides Reaction Centers....Pages 127-134
Electric Field Modulation of the Fluorescence Lineshape for Photosynthetic Reaction Centers: A New Probe of the Primary Electron Transfer Mechanism....Pages 135-140
One Step Electron Transfer to P+H? in Reaction Centers of Rhodobacter sphaeroides Derived from Dichroic Excitation Spectra of Electric Field Modulated Fluorescence Yield....Pages 141-146
Energy Transfer and Charge Separation Kinetics in the Reaction Center of Chloroflexus aurantiacus Studied by Picosecond Time-Resolved Fluorescence Spectroscopy....Pages 147-155
Analog Effects of Low Temperature and Lipid Rigidity on the Distribution of Two Conformational States of the Reaction Centers....Pages 157-168
Front Matter....Pages 169-180
Recent Advances in the Structure Analysis of Rhodopseudomonas viridis Reaction Center Mutants....Pages 181-188
Front Matter....Pages 189-196
Genetic Coding Algorithms for Engineering Membrane Proteins....Pages 197-197
EPR and ENDOR Studies of the Oxidized Donor in Reaction Centers of Rhodobacter sphaeroides Strain R-26 and two Heterodimer Mutants in which Histidine M202 or L173 was Replaced by Leucine....Pages 199-208
Site-Directed Mutations Affecting Primary Photochemistry in Reaction Centers: Effects of Dissymmetry in the Special Pair....Pages 197-197
Electrostatic Effects on the Speed and Directionality of Electron Transfer in Bacterial Reaction Centers: The Special Role of Tyrosine M-208....Pages 209-218
Transmembrane Helix Exchanges Between Quasi-Symmetric Subunits of the Photosynthetic Reaction Center....Pages 219-228
Ground State and Femtosecond Transient Absorption Spectroscopy of a Mutant of Rhodobacter capsulatus which Lacks the Initial Electron Acceptor Bacteriopheophytin....Pages 229-238
Effect of Cofactor Structure on Control of Electron Transfer Rates at the QA Site of the Reaction Center Protein....Pages 239-249
Front Matter....Pages 251-264
Energy Transfer and Trapping in Spectrally Disordered Photosynthetic Membranes....Pages 265-271
Multi-Step Electron Transfer in Rigid Photo-synthetic Models at Low Temperature: Requirements for Charge Separation and Spin-Polarized Radical Ion Pair Formation....Pages 273-282
Electronic Couplings for Light Induced Charge Transfer in Covalently Bonded Donor-Acceptor Systems....Pages 283-291
Micro-Environmental Effects on Photosynthetic Chromophores....Pages 293-302
Simulation of the Absorption and Circular Dichroism Spectra for the Primary Electron Donor in Reaction Centers of Purple Bacteria and Photosystem II....Pages 303-312
On the Primary Charge Separation in Bacterial Photosynthesis....Pages 313-326
Multiple Excited States of Photosynthetic Reaction Centers....Pages 327-336
Front Matter....Pages 337-337
An Extended Model for Electron Spin Polarization in Photosynthetic Bacteria....Pages 339-348
Can Electron Transfer be Influenced by Protein Dynamics: The Transfer from Cytochrome C to the Special Pair in Photosynthetic Reaction Centers....Pages 349-357
Front Matter....Pages 359-365
Beyond Native Reaction Centers....Pages 367-376
Back Matter....Pages 377-388
....Pages 389-400