Ebook: Cytoskeletal and Extracellular Proteins: Structure, Interactions and Assembly The 2nd International EBSA Symposium

In this volume the contributions of the 2nd International EBSA (European Biophysical Societies Association) Symposium devoted to the biophysical and biochemical aspects of the structure and interaction of cytoskeletal and extracellular proteins are presented. Topics such as supramolecular structure and organization, thermodynamics and kinetics of assembly, as well as the basic mechanisms of protein-protein interactions are discussed, and special emphasis is given to applied biophysical techniques.

---

In this volume the contributions of the 2nd International EBSA (European Biophysical Societies Association) Symposium devoted to the biophysical and biochemical aspects of the structure and interaction of cytoskeletal and extracellular proteins are presented. Topics such as supramolecular structure and organization, thermodynamics and kinetics of assembly, as well as the basic mechanisms of protein-protein interactions are discussed, and special emphasis is given to applied biophysical techniques.

---

In this volume the contributions of the 2nd International EBSA (European Biophysical Societies Association) Symposium devoted to the biophysical and biochemical aspects of the structure and interaction of cytoskeletal and extracellular proteins are presented. Topics such as supramolecular structure and organization, thermodynamics and kinetics of assembly, as well as the basic mechanisms of protein-protein interactions are discussed, and special emphasis is given to applied biophysical techniques.
Content:
Front Matter....Pages I-XIV
Front Matter....Pages 1-1
Functional Properties of ?-Tubulin In Vivo....Pages 3-8
Paracrystals of Tau Protein — A Molecular Accordion....Pages 9-14
Structure and Assembly of Intermediate Filaments: Multi- Faceted, Myosin-like (But Non-Motile) Cytoskeletal Polymers....Pages 15-26
Structure and Assembly of Calf Hoof Keratin Filaments....Pages 27-32
Structure and Assembly of Neurofilaments (NF): Analysis of Native NF and of Specific NF Subunit Combinations....Pages 33-38
Mitochondrial Creatine Kinase (Mi-CK) Forms Octameric Molecules: Structure-Function Relationship and Implications for the CP-Shuttle....Pages 39-41
Current State of the Structural Analysis of the Actin: DNase I Complex....Pages 42-47
The Structure of F-Actin Calculated from X-Ray Fibre Diagrams and the 0.6 nm Crystal Structure....Pages 48-50
The “Lateral Slipping” Model of F-Actin Filaments....Pages 51-53
A New Model for Actin — A Rigid Helical Backbone with Flexible Outer Domains....Pages 54-56
Biochemical and Structural Analysis of the Interaction of ?-Actinin with Actin Filaments and Lipids....Pages 57-59
Functional Analysis of Actin Binding Proteins In Vitro and In Vivo....Pages 60-61
Comparison of Various Gelsolin-Like Proteins and Their Interaction with Actin Filaments....Pages 62-64
Front Matter....Pages 65-66
Basement Membrane (Type IV) Collagen — Its Molecular and Macromolecular Structure....Pages 67-67
Effects of Mutations that Change Primary Structure of Collagen on the Self-Assembly of the Protein into Fibrils....Pages 69-80
Molecular Cloning of Chicken Type VI Collagen....Pages 81-89
Structure and Biology of the Laminin-Nidogen Complex....Pages 90-91
Structure of the Basement Membrane Protein Laminin: Variations on a Theme....Pages 92-101
Triple Coiled-Coil Regions of Laminin: Specificity and Chain Stability....Pages 102-105
Front Matter....Pages 106-107
A Fragment of Laminin Comprising the Entire Long Arm....Pages 67-67
5?-Nucleotidase as Receptor for the Extracellular Matrix Proteins Laminin and Fibronectin....Pages 108-109
Monoclonal Antibody 127 Recognizes a Subpopulation of Chicken Hexabrachion/Tenascin....Pages 110-113
Functional Domains of Tenascin....Pages 114-116
Complex Formation of Endothelial Cell Thrombospondin with Heparin and Fibronectin....Pages 117-118
Functional Aspects of Proteoglycan Domain Structure....Pages 119-122
Front Matter....Pages 123-133
Multi-Domain Proteins: Towards Complete Structures....Pages 135-135
Structure and Spatial Organisation of Intermediate Filament and Nuclear Lamin Molecules....Pages 137-139
The Role of Repeating Sequence Motifs in Interactions Between ?-Helical Coiled-Coils such as Myosin, Tropomyosin and Intermediate-Filament Proteins....Pages 140-149
The Self-Assembly of Nonsarcomeric Myosins....Pages 150-159
Shape Changes in Myosin Induced by the Regulatory Light Chain Subunits....Pages 160-162
Plectin/Vimentin Interaction: Molecular Binding Domains and Regulation by Phosphorylation....Pages 163-165
Interaction of Phosphoproteins with Calcium Phosphate....Pages 166-168
Thermal Stability and Folding of the Triple Helices of Interstitial and Basement Membrane Collagens....Pages 169-170
Refolding of Collagen IV and Other Collagens as Monitored by Electron Microscopy....Pages 171-181
Front Matter....Pages 182-183
Scanned and Fixed Beam Microscopy of Cytoskeletal Components....Pages 185-185
Diffraction Studies of Glycated Collagen....Pages 187-202
Oscillations in Microtubule Assembly Studied by Time-Resolved X-Ray Scattering....Pages 203-211
Study of the Secondary Structure of Tubulin in Solution and in Microtubules by Raman Spectroscopy....Pages 212-221
Light Scattering Techniques....Pages 222-224
Front Matter....Pages 225-227
Chain Dynamics, Transport and Structural Properties of Actin Networks: A Quasielastic Light Scattering and Microfluorescence Study....Pages 185-185
Polymerization of Actin on Supported Lipid Bilayers....Pages 228-232
Macrophage Intracellular Motility and Its Correlation with Cell-Substrate Adhesion....Pages 233-234
Adhesion of Pulmonary Macrophages to Langmuir-Blodgett Films, Investigated by Interference Reflection Microscopy....Pages 235-243
Front Matter....Pages 244-245
Microtubule Dynamics: Experimental Evidence and Numerical Modelling....Pages 247-247
The Effects of Magnesium on the Dynamic Instability of Individual Microtubules....Pages 249-258
About the Recognition of Colchicine by Tubulin....Pages 259-261
Phosphate Release Following Nucleotide Hydrolysis Regulates the Dynamics of Actin Filaments and Microtubules....Pages 262-263
Self-Assembly of Supramolecular Structures: The Role of Fluctuations....Pages 264-267
Structure-Function Studies of the Actin Filament System of Acanthamoeba....Pages 268-270
Equilibrium and Kinetics of the Assembly of Capping Proteins with the Ends of Actin Filaments....Pages 271-279
Lithium, Thiocyanate and Actin Assembly....Pages 280-288
Procollagen Processing Control of Type I Collagen Fibril Assembly....Pages 289-291
Supramolecular Organization of Extracellular Matrices: Analogy with Liquid Crystals....Pages 292-301
Front Matter....Pages 302-304
A Modular Model of the Nuclear Pore Complex....Pages 305-305
Strategies in Regulation of Protein Associations of the Spectrin-Based Membrane Skeleton....Pages 307-311
A cAmp-Dependent Phosphorylation Alters Spectrin Binding Properties....Pages 312-315
Inhibition of Tropomyosin Binding to F-Actin by Tropomodulin, a New Tropomyosin-Binding Protein from the Human Erythrocyte Membrane....Pages 316-318
Front Matter....Pages 319-327
A Model for Protein-Protein Interactions Involved in the Linkage of the Actin Cytoskeleton to Transmembrane Receptors for Extracellular Matrix Proteins....Pages 328-336
The Anchorage of the Cytoskeleton to the Plasma Membrane: Interaction of Vinculin with Membranes In Vitro and In Situ....Pages 337-340
Connectors of Supramolecular Assemblies....Pages 305-305
Basement Membrane Synthesis in Drosophila: Indications for more than One Assembly Mechanism for Basement Membranes....Pages 341-344
Laminin Polymerization and Binding to Glycosaminoglycans: A Hypothesis for Modulation of Basement Membrane Structure....Pages 345-346
....Pages 347-354