Ebook: Protein Structure and Engineering

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Content:
Front Matter....Pages i-ix
Introduction....Pages 1-2
Fundamentals of Neutron Diffraction....Pages 3-33
Purification of Complexes between Peptide Antigens and Class II Major Histocompatibility Complex Antigens Using Biotinylated Peptides....Pages 35-48
NMR Method for Protein Structure Determination in Solution....Pages 49-59
Determination of Structural Uncertainty from NMR and Other Data: The Lac Repressor Headpiece....Pages 61-68
The Generation of Three-Dimensional Structures from NMR-Derived Constraints....Pages 69-78
2D-NMR for 3D-Structure of Membrane Spanning Polypeptides: Gramacidin A and Fragments of Bacteriorhodopsin....Pages 79-95
The Dynamics of Oligonucleotides and Peptides Determined by Proton NMR....Pages 97-109
Methods of Stable-Isotope-Assisted Protein NMR Spectroscopy in Solution....Pages 111-138
NMR Studies of Protein Dynamics and Folding....Pages 139-154
Understanding the Specificity of the Dihydrofolate Reductase Binding Site....Pages 155-192
Histone H1 Solution Structure and the Sealing of Mammalian Nucleosome....Pages 193-207
Spectroscopy of Molecular Structure and Dynamics....Pages 209-220
Molecular Dynamics: Applications to Proteins....Pages 221-241
Protein Engineering and Biophysical Studies of Metal Binding Proteins....Pages 243-256
Growth Hormones: Expression, Structure and Protein Engineering....Pages 257-267
Site-Directed Mutagenesis and the Mechanism of Flavoprotein Disulphide Oxidoreductases....Pages 269-290
Resonance Raman and Site-Directed Mutagenesis Studies of Myoglobin Dynamics....Pages 291-308
Comparison of the Secondary Structures of Human Class I and Class II MHC Antigens by Ftir and CD Spectroscopy....Pages 309-324
Specificities of Germ Line Antibodies....Pages 325-331
Back Matter....Pages 333-346
....Pages 347-354