Ebook: The Photosynthetic Bacterial Reaction Center: Structure and Dynamics

This volume contains the contributions from the speakers at the NATO Advanced Research Workshop on "Structure of the Photosynthetic Bacterial Reaction Center X-ray Crystallography and Optical Spectroscopy with Polarized Light" which was held at the "Maison d'Hotes" of the Centre d'Etudes Nucleaires de Cadarache in the South of France, 20-25 September, 1987. This meeting continued in the spirit of a previous workshop which took place in Feldafing (FRG), March 1985. Photosynthetic reaction centers are intrinsic membrane proteins which, by performing a photoinduced transmembrane charge separation, are responsible for the conversion and storage of solar energy. Since the pioneering work of Reed and Clayton (1968) on the isolation of the reaction center from photosynthetic bacteria, optical spectroscopy with polarized light has been one of the main tools used to investigate the geometrical arrangement of the various chromophores in these systems. The recent elucidation by X-ray crystallography of the structure of several bacterial reaction centers, a breakthrough initiated by Michel and Deisenhofer, has provided us with the atomic coordinates of the pigments and some details about their interactions with neighboring aminoacid residues. This essential step has given a large impetus both to experimentalists and to theoreticians who are now attempting to relate the X-ray structural model to the optical properties of the reaction center and ultimately to its primary biological function.

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Content:
Front Matter....Pages i-x
Structure of the Reaction Center from Rhodobacter sphaeroides R-26 and 2.4.1....Pages 1-3
Crystallographic Studies of the Photosynthetic Reaction Center from Wild Type Rhodobacter sphaeroides (Y Strain)....Pages 5-11
Single Crystals of the Photochemical Reaction Center from Rhodobacter sphaeroides Wild Type Strain 2.4.1 Analyzed by Polarized Light....Pages 13-20
Protein-prosthetic Group Interactions in Bacterial Reaction Centers....Pages 21-25
Circular Dichroism Spectroscopy of Photoreaction Centers....Pages 27-32
Anisotropic Magnetic Field Effects of the Photosynthetic Bacterial Reaction Center of Rhodobacter sphaeroides R-26, Studied by Linear Dichroic Magneto-optical Difference Spectroscopy (LD-MODS) in the Temperature Range 1.2 – 310 K....Pages 33-39
Bacterial Reaction Centers are Intrinsically Heterogeneous....Pages 41-50
Reaction Centers of Purple Bacteria with Modified Chromophores....Pages 51-57
Quantitative Analysis of Genetically Altered Reaction Centers Using an In Vitro Cytochrome Oxidation Assay....Pages 59-69
Structural and Functional Properties of the Reaction Center of Green Bacteria and Heliobacteria....Pages 71-88
The Stark Effect in Photosynthetic Reaction Centers from Rhodobacter sphaeroides R-26, Rhodopseudomonas viridis and the D1D2 Complex of Photosystem II from Spinach....Pages 89-99
The Nature of Excited States and Intermediates in Bacterial Photosynthesis....Pages 101-111
The Primary Electron Transfer in Photosynthetic Purple Bacteria: Long Range Electron Transfer in the Femtosecond Domain at Low Temperature....Pages 113-118
The Problem of Primary Energy Conversion in Reaction Centers of Photosynthetic Bacteria....Pages 119-127
Temperature Effects on the Ground State Absorption Spectra and Electron Transfer Kinetics of Bacterial Reaction Centers....Pages 129-138
ENDOR of Exchangeable Protons of the Reduced Intermediate Acceptor in Reaction Centers from Rhodobacter sphaeroides R-26....Pages 139-150
FTIR Spectroscopic Investigations of the Intermediary Electron Acceptor Photoreduction in Purple Photosynthetic Bacteria and Green Plants....Pages 151-164
Charge Recombination at Low Temperature in Photosynthetic Bacteria Reaction Centers....Pages 165-176
Pressure Effects on Electron Transfer in Bacterial Reaction Centers....Pages 177-183
The Spectral Properties of Chlorophyll and Bacteriochlorophyll Dimers; a Comparative Study....Pages 185-194
Spectroscopic Properties and Electron Transfer Dynamics of Reaction Centers....Pages 195-203
Analysis of A,LD,CD,ADMR and LD-ADMR Spectra for the Reaction Centers of Rps.viridis, Rb.sphaeroides, C.aurantiacus and Modified Rb.sphaeroides....Pages 205-218
Modified-CI Model of Protein-induced Red Shifts of Reaction Center Pigment Spectra....Pages 219-228
Discussion of the Large Homogeneous Width of the P-Band in Bacterial- and Plant Reaction Centers....Pages 229-235
Theoretical Models of Electrochromic and Environmental Effects on Bacterio-Chlorophylls and -Pheophytins in Reaction Centers....Pages 237-250
Early Steps in Bacterial Photosynthesis. Comparison of Three Mechanisms....Pages 251-258
Mechanism of the Primary Charge Separation in Bacterial Photosynthetic Reaction Centers....Pages 259-269
A Configuration Interaction (CI) Description of Vectorial Electron Transfer in Bacterial Reaction Centres....Pages 271-287
Charge Transfer States and the Mechanism of Charge Separation in Bacterial Reaction Centers....Pages 289-293
Light Reflections....Pages 295-307
Back Matter....Pages 309-317
....Pages 319-329