Ebook: Biophysics and Synchrotron Radiation

This book the second volume in the "Springer Series in Biophysics" col­ lects together contributions to the conference on "Biophysics and Syn­ chrotron Radiation" held in July 86 at Frascati. This meeting addressed the advances on the structure of biological molecules obtained by using synchrotron radiation. In fact it was most timely to review the results of the research in biophysics which is rapidly developing at synchrotron radiation facilities. Moreover, there was interest to discuss the new perspectives opened up by the future high brilliance synchrotron radia­ tion sources. With the use of synchrotron radiation, x-ray spectroscopy of biological molecules is firmly established in the techniques of EXAFS and XANES. Contributions to the detailed knowledge of local structure of active sites of metalloproteins by this approach are presented in this volume, together with a number of studies of -the interaction of metal ions with other important biological macromolecular systems. Structural determination of very large biological systems at high reso­ lution, including a protein and its substrate, are reported. The experi­ mental advances in protein crystallography presented here reduce the time for solving protein structures, thus satisfying a major require­ ment of the rapidly-expanding field of protein engineering.

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This book, the second volume in the "Springer Series in Biophysics", contains contributions to the conference on Biophysics and Synchrotron Radiation held in July 1986 at Frascati. It is devoted to advances in the resolution of biological molecule structure obtainable through synchroton radiation studies. Moreover, new perspectives are opened up by the future high brilliance synchroton radiation sources. The use of synchroton radiation has firmly established x-ray spectroscopy of biological molecules. More detailed knowledge on the local structure of active sites of metalloproteins, as well as a number of studies on the interaction of metal ions with other important biological macromolecular systems are presented. This new method for protein structure analysis is a major improvement for the rapidly expanding field of protein engineering.

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This book, the second volume in the "Springer Series in Biophysics", contains contributions to the conference on Biophysics and Synchrotron Radiation held in July 1986 at Frascati. It is devoted to advances in the resolution of biological molecule structure obtainable through synchroton radiation studies. Moreover, new perspectives are opened up by the future high brilliance synchroton radiation sources. The use of synchroton radiation has firmly established x-ray spectroscopy of biological molecules. More detailed knowledge on the local structure of active sites of metalloproteins, as well as a number of studies on the interaction of metal ions with other important biological macromolecular systems are presented. This new method for protein structure analysis is a major improvement for the rapidly expanding field of protein engineering.
Content:
Front Matter....Pages I-XII
Front Matter....Pages 1-1
The Impact of Synchrotron Radiation on Protein Crystallography....Pages 3-9
Crystallography of the Complex tRNA Asp-Aspartyl-tRNA Synthetase from Yeast....Pages 10-18
Time-Resolved X-Ray Fibre Diffraction Studies of Structural Transitions in the DNA Double-Helix Using the Daresbury SRS....Pages 19-31
Multiwavelength Anomalous Diffraction in Protein Crystallography: Phase Calculations, Experimental Procedures and Results....Pages 32-40
X-Ray Diffuse Scattering from Protein Crystals....Pages 41-46
Laue Diffraction from Protein Crystals: Theoretical Aspects....Pages 47-51
White Beam Laue Diffraction: Data Processing Developments and Results for Single Crystal Data....Pages 52-60
A New Area Detector (Imaging Plate) and Its Application to Diffraction Studies at the Photon Factory....Pages 61-72
High Voltage Electron Diffraction and Computer Graphics of Heavy Meromyosin Crystals....Pages 73-78
Front Matter....Pages 79-79
Higher Order Correlations in Proteins by X-Ray Spectroscopy....Pages 81-88
Oxygen Binding Site Structure in Hemoproteins by XANES....Pages 89-98
Local Structure of Heme-Iron Studied by High-Resolution XANES: Thermal Spin Equilibrium in Myoglobin....Pages 99-106
The High and Low Spin Iron Sites and the T/to R/Transition in Carp Hemoglobin....Pages 107-113
EXAFS and XANES Studies of High-Valent Metal-Oxo Porphyrins Relevant to Horseradish Peroxidase....Pages 114-121
Energy Dispersive X-Ray Absorption Spectroscopy: Strengths and Limitations for Time-Resolved Studies of Biostructures....Pages 122-129
A Rapid-Freeze EXAFS Method for the Studies of Intermediates in Fast Enzymatic Reactions....Pages 130-135
EXAFS and Structure-Function Relationships in Zinc Proteins....Pages 136-146
EXAFS and XANES Studies of Copper Proteins....Pages 147-158
The Active Site of Cu,Zn Superoxide Dismutase as Studied by EXAFS: The Binding of Chloride to the Reduced Enzyme....Pages 159-163
Structural Changes in Hemerythrin and Error Analysis....Pages 164-170
Front Matter....Pages 79-79
The Interaction of Iron With Transferrins....Pages 171-175
Structural Features of the Metal Binding Sites in Transferrins by EXAFS and Other Spectroscopic Techniques....Pages 176-184
Applications of X-Ray Absorption Spectroscopy to the Study of Ferritin....Pages 185-190
Structural Investigations of Biomimetic Complexes of Cytochrome P-450 by Difference EXAFS Spectroscopy....Pages 191-200
Investigation on Metal Binding Sites in DNA by Means of X-Ray High Resolution Spectroscopy....Pages 201-211
Study of Cu-ATP Complexes....Pages 212-222
X-Ray Absorption Spectroscopy of Manganese and Iron in the Photosynthetic Apparatus....Pages 223-230
EXAFS and XANES Studies of Calcium Polysaccharides....Pages 231-237
Application of X-Ray Absorption Spectroscopy to the Investigation of Biological Calcification....Pages 238-245
Ca Site Structure in Bungarotoxin: Possible Generalization to Ca Low Affinity and High Specificity Sites in Ca Binding Proteins....Pages 246-253
Front Matter....Pages 255-255
Resonant X-Ray Scattering of Biological Systems....Pages 257-272
Chromatin, Solution Scattering and Perception....Pages 273-280
Pre-Nucleation and Nucleation Events in Microtubule Assembly: A Time-Resolved X-Ray Scattering Study....Pages 281-294
Sinusoidal Length Change Study of Muscle Contraction and Self-Induced Translation Model of Myosin Motion....Pages 295-302
Small Angle X-Ray Diffraction of Collagen Fibrils Using a Three-Dimensional Imaging Gas Detector....Pages 303-308
Front Matter....Pages 309-309
X-Ray Microscopy at the Electron Storage Ring BESSY....Pages 311-317
Front Matter....Pages 319-319
Quaternary Structure Changes in Aspartokinase-Homoserine Dehydrogenase Studied by Time-Resolved Fluorescence....Pages 321-327
Lifetime of Fluorophores Excited State in Biological Membranes and Their Model Systems....Pages 328-333

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This book, the second volume in the "Springer Series in Biophysics", contains contributions to the conference on Biophysics and Synchrotron Radiation held in July 1986 at Frascati. It is devoted to advances in the resolution of biological molecule structure obtainable through synchroton radiation studies. Moreover, new perspectives are opened up by the future high brilliance synchroton radiation sources. The use of synchroton radiation has firmly established x-ray spectroscopy of biological molecules. More detailed knowledge on the local structure of active sites of metalloproteins, as well as a number of studies on the interaction of metal ions with other important biological macromolecular systems are presented. This new method for protein structure analysis is a major improvement for the rapidly expanding field of protein engineering.
Content:
Front Matter....Pages I-XII
Front Matter....Pages 1-1
The Impact of Synchrotron Radiation on Protein Crystallography....Pages 3-9
Crystallography of the Complex tRNA Asp-Aspartyl-tRNA Synthetase from Yeast....Pages 10-18
Time-Resolved X-Ray Fibre Diffraction Studies of Structural Transitions in the DNA Double-Helix Using the Daresbury SRS....Pages 19-31
Multiwavelength Anomalous Diffraction in Protein Crystallography: Phase Calculations, Experimental Procedures and Results....Pages 32-40
X-Ray Diffuse Scattering from Protein Crystals....Pages 41-46
Laue Diffraction from Protein Crystals: Theoretical Aspects....Pages 47-51
White Beam Laue Diffraction: Data Processing Developments and Results for Single Crystal Data....Pages 52-60
A New Area Detector (Imaging Plate) and Its Application to Diffraction Studies at the Photon Factory....Pages 61-72
High Voltage Electron Diffraction and Computer Graphics of Heavy Meromyosin Crystals....Pages 73-78
Front Matter....Pages 79-79
Higher Order Correlations in Proteins by X-Ray Spectroscopy....Pages 81-88
Oxygen Binding Site Structure in Hemoproteins by XANES....Pages 89-98
Local Structure of Heme-Iron Studied by High-Resolution XANES: Thermal Spin Equilibrium in Myoglobin....Pages 99-106
The High and Low Spin Iron Sites and the T/to R/Transition in Carp Hemoglobin....Pages 107-113
EXAFS and XANES Studies of High-Valent Metal-Oxo Porphyrins Relevant to Horseradish Peroxidase....Pages 114-121
Energy Dispersive X-Ray Absorption Spectroscopy: Strengths and Limitations for Time-Resolved Studies of Biostructures....Pages 122-129
A Rapid-Freeze EXAFS Method for the Studies of Intermediates in Fast Enzymatic Reactions....Pages 130-135
EXAFS and Structure-Function Relationships in Zinc Proteins....Pages 136-146
EXAFS and XANES Studies of Copper Proteins....Pages 147-158
The Active Site of Cu,Zn Superoxide Dismutase as Studied by EXAFS: The Binding of Chloride to the Reduced Enzyme....Pages 159-163
Structural Changes in Hemerythrin and Error Analysis....Pages 164-170
Front Matter....Pages 79-79
The Interaction of Iron With Transferrins....Pages 171-175
Structural Features of the Metal Binding Sites in Transferrins by EXAFS and Other Spectroscopic Techniques....Pages 176-184
Applications of X-Ray Absorption Spectroscopy to the Study of Ferritin....Pages 185-190
Structural Investigations of Biomimetic Complexes of Cytochrome P-450 by Difference EXAFS Spectroscopy....Pages 191-200
Investigation on Metal Binding Sites in DNA by Means of X-Ray High Resolution Spectroscopy....Pages 201-211
Study of Cu-ATP Complexes....Pages 212-222
X-Ray Absorption Spectroscopy of Manganese and Iron in the Photosynthetic Apparatus....Pages 223-230
EXAFS and XANES Studies of Calcium Polysaccharides....Pages 231-237
Application of X-Ray Absorption Spectroscopy to the Investigation of Biological Calcification....Pages 238-245
Ca Site Structure in Bungarotoxin: Possible Generalization to Ca Low Affinity and High Specificity Sites in Ca Binding Proteins....Pages 246-253
Front Matter....Pages 255-255
Resonant X-Ray Scattering of Biological Systems....Pages 257-272
Chromatin, Solution Scattering and Perception....Pages 273-280
Pre-Nucleation and Nucleation Events in Microtubule Assembly: A Time-Resolved X-Ray Scattering Study....Pages 281-294
Sinusoidal Length Change Study of Muscle Contraction and Self-Induced Translation Model of Myosin Motion....Pages 295-302
Small Angle X-Ray Diffraction of Collagen Fibrils Using a Three-Dimensional Imaging Gas Detector....Pages 303-308
Front Matter....Pages 309-309
X-Ray Microscopy at the Electron Storage Ring BESSY....Pages 311-317
Front Matter....Pages 319-319
Quaternary Structure Changes in Aspartokinase-Homoserine Dehydrogenase Studied by Time-Resolved Fluorescence....Pages 321-327
Lifetime of Fluorophores Excited State in Biological Membranes and Their Model Systems....Pages 328-333
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