Ebook: Multidimensional Microscopy

Modern cell biology is being revolutionized by the wedding of microscopy and computers. This book describes the new instrumentation and methods which allow three-dimensional reconstruction of specimens. Multidimensional Microscopy will be of interest to cell biologists, microscopists, and basic biomedical researchers whose work involves microscopic techniques. This book presents current results on a very active field in modern biology: methods in light and electron microscopy that allow the reconstruction of three-dimensional objects with the aid of computers. The book emphasizes the methods that can be used and examples of biological systems to which they have been applied. It includes extensive descriptions of confocal microscopy and its applications, as well as chapters on X-ray microscopy, low-voltage electron microscopy, and image reconstruction. This is an impressive summary of state-of-the-art methods in microscopy, in which microscopes and computers are being joined to permit specimens to be examined and reconstructed in three dimensions. Will be of interest to cell biologists, biomedical researchers, and microscopists.

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Modern cell biology is being revolutionized by the wedding of microscopy and computers. This book describes the new instrumentation and methods which allow three-dimensional reconstruction of specimens. Multidimensional Microscopy will be of interest to cell biologists, microscopists, and basic biomedical researchers whose work involves microscopic techniques. This book presents current results on a very active field in modern biology: methods in light and electron microscopy that allow the reconstruction of three-dimensional objects with the aid of computers. The book emphasizes the methods that can be used and examples of biological systems to which they have been applied. It includes extensive descriptions of confocal microscopy and its applications, as well as chapters on X-ray microscopy, low-voltage electron microscopy, and image reconstruction. This is an impressive summary of state-of-the-art methods in microscopy, in which microscopes and computers are being joined to permit specimens to be examined and reconstructed in three dimensions. Will be of interest to cell biologists, biomedical researchers, and microscopists.

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Modern cell biology is being revolutionized by the wedding of microscopy and computers. This book describes the new instrumentation and methods which allow three-dimensional reconstruction of specimens. Multidimensional Microscopy will be of interest to cell biologists, microscopists, and basic biomedical researchers whose work involves microscopic techniques. This book presents current results on a very active field in modern biology: methods in light and electron microscopy that allow the reconstruction of three-dimensional objects with the aid of computers. The book emphasizes the methods that can be used and examples of biological systems to which they have been applied. It includes extensive descriptions of confocal microscopy and its applications, as well as chapters on X-ray microscopy, low-voltage electron microscopy, and image reconstruction. This is an impressive summary of state-of-the-art methods in microscopy, in which microscopes and computers are being joined to permit specimens to be examined and reconstructed in three dimensions. Will be of interest to cell biologists, biomedical researchers, and microscopists.
Content:
Front Matter....Pages i-xvii
Confocal Microscopy: Basic Principles and System Performance....Pages 1-31
Designing a Confocal Fluorescence Microscope....Pages 33-51
Theory and Principal Technologies of the Laser Scanning Confocal Microscope....Pages 53-71
Simultaneous Ultraviolet and Visible Excitation Confocal Microscopy....Pages 73-85
High Resolution Confocal Microscopy of Phase and Amplitude Objects....Pages 87-102
Interferometric Microscopy — An Overview of the Optical Characterization of Inertial-fusion Targets....Pages 103-121
X-Ray Microscopy — Possibilities for Three Dimensional Imaging....Pages 123-132
High Resolution Projection X-Ray Microscopy....Pages 133-150
Cone-beam X-ray Microtomography....Pages 151-169
High Resolution Low Voltage Scanning Electron Microscopy: Reduced Radiation Damage on Cryo-specimens....Pages 171-190
Image Restoration in Light Microscopy....Pages 191-208
Image Quality Considerations in Computerized 2-D and 3-D Microscopy....Pages 209-230
Analysis and Presentation of Three Dimensional Data Sets....Pages 231-250
The Processing of Three-dimensional Confocal Data Sets....Pages 251-266
Effective Laser Lines for Biological Confocal Microscopy....Pages 267-289
Visualizing DNA Replication in Three Dimensions....Pages 291-303
Study of Nuclear Proteins Associated with Cell Proliferation, Differentiation, and Mitosis by Confocal Fluorescence Microscopy....Pages 305-319
Two- and Three-Dimensional Confocal Microscopy of the Eye....Pages 321-338
Confocal Microscopy of Botanical Specimens....Pages 339-380
Back Matter....Pages 381-387

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Modern cell biology is being revolutionized by the wedding of microscopy and computers. This book describes the new instrumentation and methods which allow three-dimensional reconstruction of specimens. Multidimensional Microscopy will be of interest to cell biologists, microscopists, and basic biomedical researchers whose work involves microscopic techniques. This book presents current results on a very active field in modern biology: methods in light and electron microscopy that allow the reconstruction of three-dimensional objects with the aid of computers. The book emphasizes the methods that can be used and examples of biological systems to which they have been applied. It includes extensive descriptions of confocal microscopy and its applications, as well as chapters on X-ray microscopy, low-voltage electron microscopy, and image reconstruction. This is an impressive summary of state-of-the-art methods in microscopy, in which microscopes and computers are being joined to permit specimens to be examined and reconstructed in three dimensions. Will be of interest to cell biologists, biomedical researchers, and microscopists.
Content:
Front Matter....Pages i-xvii
Confocal Microscopy: Basic Principles and System Performance....Pages 1-31
Designing a Confocal Fluorescence Microscope....Pages 33-51
Theory and Principal Technologies of the Laser Scanning Confocal Microscope....Pages 53-71
Simultaneous Ultraviolet and Visible Excitation Confocal Microscopy....Pages 73-85
High Resolution Confocal Microscopy of Phase and Amplitude Objects....Pages 87-102
Interferometric Microscopy — An Overview of the Optical Characterization of Inertial-fusion Targets....Pages 103-121
X-Ray Microscopy — Possibilities for Three Dimensional Imaging....Pages 123-132
High Resolution Projection X-Ray Microscopy....Pages 133-150
Cone-beam X-ray Microtomography....Pages 151-169
High Resolution Low Voltage Scanning Electron Microscopy: Reduced Radiation Damage on Cryo-specimens....Pages 171-190
Image Restoration in Light Microscopy....Pages 191-208
Image Quality Considerations in Computerized 2-D and 3-D Microscopy....Pages 209-230
Analysis and Presentation of Three Dimensional Data Sets....Pages 231-250
The Processing of Three-dimensional Confocal Data Sets....Pages 251-266
Effective Laser Lines for Biological Confocal Microscopy....Pages 267-289
Visualizing DNA Replication in Three Dimensions....Pages 291-303
Study of Nuclear Proteins Associated with Cell Proliferation, Differentiation, and Mitosis by Confocal Fluorescence Microscopy....Pages 305-319
Two- and Three-Dimensional Confocal Microscopy of the Eye....Pages 321-338
Confocal Microscopy of Botanical Specimens....Pages 339-380
Back Matter....Pages 381-387
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