Ebook: Applied Microbiology and Molecular Biology in Oilfield Systems: Proceedings from the International Symposium on Applied Microbiology and Molecular Biology in Oil Systems (ISMOS-2), 2009

Applied Microbiology and Molecular Biology in Oil Field Systems addresses the major problems microbes cause in oil fields, (e.g. biocorrosion and souring) and how beneficial microbial activities may be exploited (e.g. MEOR and biofuels). The book describes theoretical and practical approaches to specific Molecular Microbiological Methods (MMM), and is written by leading authorities in the field from both academia and industry. The book describes how MMM can be applied to faciliate better management of oil reservoirs and downstream processes. The book is innovative in that it utilises real industrial case studies which gives useful technical and scientific information to researchers, engineers and microbiologists working with oil, gas and petroleum systems.

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Applied Microbiology and Molecular Biology in Oil Field Systems addresses the major problems microbes cause in oil fields, (e.g. biocorrosion and souring) and how beneficial microbial activities may be exploited (e.g. MEOR and biofuels). The book describes theoretical and practical approaches to specific Molecular Microbiological Methods (MMM), and is written by leading authorities in the field from both academia and industry. The book describes how MMM can be applied to faciliate better management of oil reservoirs and downstream processes. The book is innovative in that it utilises real industrial case studies which gives useful technical and scientific information to researchers, engineers and microbiologists working with oil, gas and petroleum systems.

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Applied Microbiology and Molecular Biology in Oil Field Systems addresses the major problems microbes cause in oil fields, (e.g. biocorrosion and souring) and how beneficial microbial activities may be exploited (e.g. MEOR and biofuels). The book describes theoretical and practical approaches to specific Molecular Microbiological Methods (MMM), and is written by leading authorities in the field from both academia and industry. The book describes how MMM can be applied to faciliate better management of oil reservoirs and downstream processes. The book is innovative in that it utilises real industrial case studies which gives useful technical and scientific information to researchers, engineers and microbiologists working with oil, gas and petroleum systems.
Content:
Front Matter....Pages i-xvi
Front Matter....Pages 1-1
Introduction....Pages 3-5
Sampling and Nucleic Extraction Procedures from Oil Reservoir Samples....Pages 7-16
Front Matter....Pages 17-17
Application of Molecular Microbiological Methods to the Oil Industry to Analyse DNA, RNA and Proteins....Pages 19-26
Which Microbial Communities Are Present? Importance of Selecting Appropriate Primers and Probes for Use in Molecular Microbiological Methods (MMM) in Oilfields....Pages 27-31
Which Microbial Communities are Present? Application of PCR-DGGE: Case Study on an Oilfield Core Sample....Pages 33-43
Which Microbial Communities Are Present? Application of Clone Libraries: Syntrophic Acetate Degradation to Methane in a High-Temperature Petroleum Reservoir – Culture-Based and 16S rRNA Genes Characterisation....Pages 45-53
Which Microbial Communities Are Present? Using Fluorescence In Situ Hybridisation (FISH): Microscopic Techniques for Enumeration of Troublesome Microorganisms in Oil and Fuel Samples....Pages 55-61
Which Microbial Communities Are Present? Sequence-Based Metagenomics....Pages 63-76
How Many Microorganisms Are Present? Quantitative Reverse Transcription PCR (qRT-PCR)....Pages 77-84
How Many Microorganisms Are Present? Techniques for Enumerating Microorganisms in Oilfields....Pages 85-91
Which Members of the Microbial Communities Are Active? Microarrays....Pages 93-99
Front Matter....Pages 101-101
Problems Caused by Microbes and Treatment Strategies Monitoring and Preventing Reservoir Souring Using Molecular Microbiological Methods (MMM)....Pages 103-107
Problems Caused by Microbes and Treatment Strategies The Effect of Nitrate Injection in Oil Reservoirs – Experience with Nitrate Injection in the Halfdan Oilfield....Pages 109-115
Problems Caused by Microbes and Treatment Strategies: Monitoring Microbial Responses to Biocides; Bioassays – A Concept to Test the Effect of Biocides on both Archaea and Bacteria in Oilfield Systems....Pages 117-124
Problems Caused by Microbes and Treatment Strategies: Identification of H2S-Producing Bacteria in Corrosion Product of a Gas Pipeline....Pages 125-131
Problems Caused by Microbes and Treatment Strategies: Rapid Diagnostics of Microbiologically Influenced Corrosion (MIC) in Oilfield Systems with a DNA-Based Test Kit....Pages 133-140
Problems Caused by Microbes and Treatment Strategies Anaerobic Hydrocarbon Biodegradation and Biocorrosion: A Case Study....Pages 141-149
Problems Caused by Microbes and Treatment Strategies Health and Safety Issues from the Production of Hydrogen Sulphide....Pages 151-157
Problems Caused by Microbes and Treatment Strategies Downstream Petroleum Microbiology – An Industry Perspective....Pages 159-167
Front Matter....Pages 169-169
How Specific Microbial Communities Benefit the Oil Industry: Biorefining and Bioprocessing for Upgrading Petroleum Oil....Pages 171-178
Front Matter....Pages 169-169
How Specific Microbial Communities Benefit the Oil Industry: Microbial-Enhanced Oil Recovery (MEOR)....Pages 179-187
How Specific Microbial Communities Benefit the Oil Industry: Anaerobic Microbial Processes and the Prospect for Methane Production from Oil....Pages 189-192
How Specific Microbial Communities Benefit the Oil Industry: Case Study – Proof of Concept that Oil Entrained in Marginal Reservoirs Can Be Bioconverted to Methane Gas as a Green Energy Recovery Strategy....Pages 193-198
How Specific Microbial Communities Benefit the Oil Industry: Dynamics of Alcanivorax spp. in Oil-Contaminated Intertidal Beach Sediments Undergoing Bioremediation....Pages 199-209
How Specific Microbial Communities Benefit the Oil Industry: Significant Contribution of Methyl/Methanol-Utilising Methanogenic Pathway in a Subsurface Biogas Environment....Pages 211-216
Front Matter....Pages 217-217
Fuel for the Future: Development of New Fuels, e.g. Biofuels....Pages 219-228
Fuel for the Future: Biodiesel – A Case study....Pages 229-235
Fuel for the Future: Unlocking New Fuel Resources....Pages 237-244
Back Matter....Pages 245-279

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Applied Microbiology and Molecular Biology in Oil Field Systems addresses the major problems microbes cause in oil fields, (e.g. biocorrosion and souring) and how beneficial microbial activities may be exploited (e.g. MEOR and biofuels). The book describes theoretical and practical approaches to specific Molecular Microbiological Methods (MMM), and is written by leading authorities in the field from both academia and industry. The book describes how MMM can be applied to faciliate better management of oil reservoirs and downstream processes. The book is innovative in that it utilises real industrial case studies which gives useful technical and scientific information to researchers, engineers and microbiologists working with oil, gas and petroleum systems.
Content:
Front Matter....Pages i-xvi
Front Matter....Pages 1-1
Introduction....Pages 3-5
Sampling and Nucleic Extraction Procedures from Oil Reservoir Samples....Pages 7-16
Front Matter....Pages 17-17
Application of Molecular Microbiological Methods to the Oil Industry to Analyse DNA, RNA and Proteins....Pages 19-26
Which Microbial Communities Are Present? Importance of Selecting Appropriate Primers and Probes for Use in Molecular Microbiological Methods (MMM) in Oilfields....Pages 27-31
Which Microbial Communities are Present? Application of PCR-DGGE: Case Study on an Oilfield Core Sample....Pages 33-43
Which Microbial Communities Are Present? Application of Clone Libraries: Syntrophic Acetate Degradation to Methane in a High-Temperature Petroleum Reservoir – Culture-Based and 16S rRNA Genes Characterisation....Pages 45-53
Which Microbial Communities Are Present? Using Fluorescence In Situ Hybridisation (FISH): Microscopic Techniques for Enumeration of Troublesome Microorganisms in Oil and Fuel Samples....Pages 55-61
Which Microbial Communities Are Present? Sequence-Based Metagenomics....Pages 63-76
How Many Microorganisms Are Present? Quantitative Reverse Transcription PCR (qRT-PCR)....Pages 77-84
How Many Microorganisms Are Present? Techniques for Enumerating Microorganisms in Oilfields....Pages 85-91
Which Members of the Microbial Communities Are Active? Microarrays....Pages 93-99
Front Matter....Pages 101-101
Problems Caused by Microbes and Treatment Strategies Monitoring and Preventing Reservoir Souring Using Molecular Microbiological Methods (MMM)....Pages 103-107
Problems Caused by Microbes and Treatment Strategies The Effect of Nitrate Injection in Oil Reservoirs – Experience with Nitrate Injection in the Halfdan Oilfield....Pages 109-115
Problems Caused by Microbes and Treatment Strategies: Monitoring Microbial Responses to Biocides; Bioassays – A Concept to Test the Effect of Biocides on both Archaea and Bacteria in Oilfield Systems....Pages 117-124
Problems Caused by Microbes and Treatment Strategies: Identification of H2S-Producing Bacteria in Corrosion Product of a Gas Pipeline....Pages 125-131
Problems Caused by Microbes and Treatment Strategies: Rapid Diagnostics of Microbiologically Influenced Corrosion (MIC) in Oilfield Systems with a DNA-Based Test Kit....Pages 133-140
Problems Caused by Microbes and Treatment Strategies Anaerobic Hydrocarbon Biodegradation and Biocorrosion: A Case Study....Pages 141-149
Problems Caused by Microbes and Treatment Strategies Health and Safety Issues from the Production of Hydrogen Sulphide....Pages 151-157
Problems Caused by Microbes and Treatment Strategies Downstream Petroleum Microbiology – An Industry Perspective....Pages 159-167
Front Matter....Pages 169-169
How Specific Microbial Communities Benefit the Oil Industry: Biorefining and Bioprocessing for Upgrading Petroleum Oil....Pages 171-178
Front Matter....Pages 169-169
How Specific Microbial Communities Benefit the Oil Industry: Microbial-Enhanced Oil Recovery (MEOR)....Pages 179-187
How Specific Microbial Communities Benefit the Oil Industry: Anaerobic Microbial Processes and the Prospect for Methane Production from Oil....Pages 189-192
How Specific Microbial Communities Benefit the Oil Industry: Case Study – Proof of Concept that Oil Entrained in Marginal Reservoirs Can Be Bioconverted to Methane Gas as a Green Energy Recovery Strategy....Pages 193-198
How Specific Microbial Communities Benefit the Oil Industry: Dynamics of Alcanivorax spp. in Oil-Contaminated Intertidal Beach Sediments Undergoing Bioremediation....Pages 199-209
How Specific Microbial Communities Benefit the Oil Industry: Significant Contribution of Methyl/Methanol-Utilising Methanogenic Pathway in a Subsurface Biogas Environment....Pages 211-216
Front Matter....Pages 217-217
Fuel for the Future: Development of New Fuels, e.g. Biofuels....Pages 219-228
Fuel for the Future: Biodiesel – A Case study....Pages 229-235
Fuel for the Future: Unlocking New Fuel Resources....Pages 237-244
Back Matter....Pages 245-279
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