Ebook: Gas Chromatography of Steroids in Biological Fluids: Proceedings of the Workshop on Gas-Liquid Chromatography of Steroids in Biological Fluids

During the past decade we have witnessed a revolution in analytical methods. The development of vapor phase chromatography for the separation and analysis of classes of substances ranging from metals and gases to a wide variety of organic materials has been one of the most exciting of these new techniques. Gas-liquid chromatography for the measurement of steroids is particularly significant for endocrinologists and reports during the past several years have demonstrated its usefulness. Because of the growing interest in this method, a committee of the Endocrinology Study Section composed of Drs. R. M. Dodson, Seymour Lieberman, Hilton A. Salhanick, and Ralph E. Peterson, felt that the time was propitious to hold this Workshop and it is on their behalf that I welcome you. We hope to obtain enough data during these sessions so that those attending this conference and those who may read the proceedings will be able to make an informed judgement about the usefulness of gas-liquid chromatography for the analysis of steroids in biological fluids. Thus, I hope that there will be adequate documentation of the reliability of the methods as well as a comparison of the advantages and disadvantages of this analytical method with other classical methods. If we can do this, this Workshop will provide a significant base of practical considerations about gas chromatographic analytic techniques. I would like to thank Drs. T. F. Gallagher, H. Wilson, H. Salhanick and L. Engel for agreeing to serve as Chairmen of the sessions.

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Content:
Front Matter....Pages i-xix
The Specificity of a Gas Chromatographic Method for the Determination of Urinary Androsterone, Etiocholanolone and Dehydroepiandrosterone in Urine....Pages 1-9
The Indirect Analysis of Urinary 17-Oxygenated C19 Steroid Sulphates by Gas-Liquid Chromatography....Pages 11-15
A Technic for Fractionation of 17-Ketosteroids from Urine....Pages 17-18
Gas Chromatographic Method for the Determination of Testosterone in Human Urine....Pages 19-22
Estimation of Testosterone in Blood by Gas-Liquid Chromatography....Pages 23-34
The Estimation of Plasma Androgens by Gas-Liquid Chromatography....Pages 35-42
The Estimation of Unconjugated Cortisol Metabolites in Urine by Gas Chromatography....Pages 57-65
Gas Chromatography of Cortols and Cortolones....Pages 67-72
Urinary Corticoids by Gas Chromatography....Pages 73-88
A Specific Method for the Gas Chromatographic Determination of Aldosterone and Adrenocortical Steriods: Application to Adrenal Venous Blood of Vertebrates....Pages 89-100
Analysis of Aldosterone in Urine by Double Isotope Dilution and Gas-Liquid Chromatography....Pages 101-111
Measurement of Urinary Steroids by Gas Chromatography. 3?, 17-Dihydroxypregnane-20-One and C21 Triols....Pages 127-133
Analysis of Urinary Pregnanediol and Pregnanetriol by Gas-Liquid Chromatography....Pages 135-142
The Application of Gas-Liquid Chromatography to the Measurement of Plasma Progesterone....Pages 143-151
Determination of Progesterone in Human Peripheral Blood Using Gas-Liquid Chromatography with Electron Capture Detection....Pages 153-168
The Analysis of Plasma Progestins by Gas Chromatography....Pages 169-176
Gas Chromatography and its Role in the Versatile Analysis of Urinary Estrogens....Pages 195-214
Determination of Urinary Estrogens by Gas Chromatography....Pages 215-228
Gas Chromatography of Estriol in Pregnancy Urine....Pages 229-232
Analysis of Estrogens in the Urine of Non-Pregnant Women by Gas Liquid Chromatography....Pages 233-236
The Measurement of Estriol in Urine and Amniotic Fluid by Gas-Liquid Chromatography....Pages 237-241
Gas Chromatography of Free and Conjugated Estrogens in Blood Plasma....Pages 243-246
Estimation of Estradiol-17? by Gas-Liquid Chromatography with Electron Capture Detection....Pages 247-253
Gas Chromatography of Estrogens and Other Steroids from Endocrine Tissues....Pages 255-261
Some Aspects of the Chemistry of Gas-Liquid Chromatography....Pages 277-295
Some Practical Problems in Gas-Liquid Chromatography....Pages 297-300
Comments on Techniques on Gas Chromatography....Pages 301-307
The Utility of GLC for Analyzing Specific Steroids in Biological Fluids....Pages 309-312
Back Matter....Pages 313-315

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Content:
Front Matter....Pages i-xix
The Specificity of a Gas Chromatographic Method for the Determination of Urinary Androsterone, Etiocholanolone and Dehydroepiandrosterone in Urine....Pages 1-9
The Indirect Analysis of Urinary 17-Oxygenated C19 Steroid Sulphates by Gas-Liquid Chromatography....Pages 11-15
A Technic for Fractionation of 17-Ketosteroids from Urine....Pages 17-18
Gas Chromatographic Method for the Determination of Testosterone in Human Urine....Pages 19-22
Estimation of Testosterone in Blood by Gas-Liquid Chromatography....Pages 23-34
The Estimation of Plasma Androgens by Gas-Liquid Chromatography....Pages 35-42
The Estimation of Unconjugated Cortisol Metabolites in Urine by Gas Chromatography....Pages 57-65
Gas Chromatography of Cortols and Cortolones....Pages 67-72
Urinary Corticoids by Gas Chromatography....Pages 73-88
A Specific Method for the Gas Chromatographic Determination of Aldosterone and Adrenocortical Steriods: Application to Adrenal Venous Blood of Vertebrates....Pages 89-100
Analysis of Aldosterone in Urine by Double Isotope Dilution and Gas-Liquid Chromatography....Pages 101-111
Measurement of Urinary Steroids by Gas Chromatography. 3?, 17-Dihydroxypregnane-20-One and C21 Triols....Pages 127-133
Analysis of Urinary Pregnanediol and Pregnanetriol by Gas-Liquid Chromatography....Pages 135-142
The Application of Gas-Liquid Chromatography to the Measurement of Plasma Progesterone....Pages 143-151
Determination of Progesterone in Human Peripheral Blood Using Gas-Liquid Chromatography with Electron Capture Detection....Pages 153-168
The Analysis of Plasma Progestins by Gas Chromatography....Pages 169-176
Gas Chromatography and its Role in the Versatile Analysis of Urinary Estrogens....Pages 195-214
Determination of Urinary Estrogens by Gas Chromatography....Pages 215-228
Gas Chromatography of Estriol in Pregnancy Urine....Pages 229-232
Analysis of Estrogens in the Urine of Non-Pregnant Women by Gas Liquid Chromatography....Pages 233-236
The Measurement of Estriol in Urine and Amniotic Fluid by Gas-Liquid Chromatography....Pages 237-241
Gas Chromatography of Free and Conjugated Estrogens in Blood Plasma....Pages 243-246
Estimation of Estradiol-17? by Gas-Liquid Chromatography with Electron Capture Detection....Pages 247-253
Gas Chromatography of Estrogens and Other Steroids from Endocrine Tissues....Pages 255-261
Some Aspects of the Chemistry of Gas-Liquid Chromatography....Pages 277-295
Some Practical Problems in Gas-Liquid Chromatography....Pages 297-300
Comments on Techniques on Gas Chromatography....Pages 301-307
The Utility of GLC for Analyzing Specific Steroids in Biological Fluids....Pages 309-312
Back Matter....Pages 313-315
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