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The optical trapping of colloidal matter is an unequalled field of technology for enabling precise handling of particles on microscopic scales, solely by the force of light. Although the basic concept of optical tweezers, which are based on a single laser beam, has matured and found a vast number of exciting applications, in particular in the life sciences, there are strong demands for more sophisticated approaches. This thesis gives an introductory overview of existing optical micromanipulation techniques and reviews the state-of-the-art of the emerging field of structured light fields and their applications in optical trapping, micromanipulation, and organisation. The author presents established, and introduces novel concepts for the holographic and non-holographic shaping of a light field. A special emphasis of the work is the demonstration of advanced applications of the thus created structured light fields in optical micromanipulation, utilising various geometries and unconventional light propagation properties. While most of the concepts developed are demonstrated with artificial microscopic reference particles, the work concludes with a comprehensive demonstration of optical control and alignment of bacterial cells, and hierarchical supramolecular organisation utilising dedicated nanocontainer particles.




The optical trapping of colloidal matter is an unequalled field of technology for enabling precise handling of particles on microscopic scales, solely by the force of light. Although the basic concept of optical tweezers, which are based on a single laser beam, has matured and found a vast number of exciting applications, in particular in the life sciences, there are strong demands for more sophisticated approaches. This thesis gives an introductory overview of existing optical micromanipulation techniques and reviews the state-of-the-art of the emerging field of structured light fields and their applications in optical trapping, micromanipulation, and organisation. The author presents established, and introduces novel concepts for the holographic and non-holographic shaping of a light field. A special emphasis of the work is the demonstration of advanced applications of the thus created structured light fields in optical micromanipulation, utilising various geometries and unconventional light propagation properties. While most of the concepts developed are demonstrated with artificial microscopic reference particles, the work concludes with a comprehensive demonstration of optical control and alignment of bacterial cells, and hierarchical supramolecular organisation utilising dedicated nanocontainer particles.


The optical trapping of colloidal matter is an unequalled field of technology for enabling precise handling of particles on microscopic scales, solely by the force of light. Although the basic concept of optical tweezers, which are based on a single laser beam, has matured and found a vast number of exciting applications, in particular in the life sciences, there are strong demands for more sophisticated approaches. This thesis gives an introductory overview of existing optical micromanipulation techniques and reviews the state-of-the-art of the emerging field of structured light fields and their applications in optical trapping, micromanipulation, and organisation. The author presents established, and introduces novel concepts for the holographic and non-holographic shaping of a light field. A special emphasis of the work is the demonstration of advanced applications of the thus created structured light fields in optical micromanipulation, utilising various geometries and unconventional light propagation properties. While most of the concepts developed are demonstrated with artificial microscopic reference particles, the work concludes with a comprehensive demonstration of optical control and alignment of bacterial cells, and hierarchical supramolecular organisation utilising dedicated nanocontainer particles.
Content:
Front Matter....Pages i-xi
Motivation and Outline....Pages 1-4
Introduction to Optical Trapping....Pages 5-26
Holographic Phase Contrast....Pages 27-45
Counter-Propagating Traps by Optical Phase-Conjugation....Pages 47-65
Non-Diffracting Beams for the Three-Dimensional Moulding of Matter....Pages 67-82
Ince-Gaussian Beams for the Optical Organisation of Microparticles....Pages 83-94
Holographic Optical Tweezers....Pages 95-116
Summary and Outlook....Pages 117-119
Back Matter....Pages 121-134


The optical trapping of colloidal matter is an unequalled field of technology for enabling precise handling of particles on microscopic scales, solely by the force of light. Although the basic concept of optical tweezers, which are based on a single laser beam, has matured and found a vast number of exciting applications, in particular in the life sciences, there are strong demands for more sophisticated approaches. This thesis gives an introductory overview of existing optical micromanipulation techniques and reviews the state-of-the-art of the emerging field of structured light fields and their applications in optical trapping, micromanipulation, and organisation. The author presents established, and introduces novel concepts for the holographic and non-holographic shaping of a light field. A special emphasis of the work is the demonstration of advanced applications of the thus created structured light fields in optical micromanipulation, utilising various geometries and unconventional light propagation properties. While most of the concepts developed are demonstrated with artificial microscopic reference particles, the work concludes with a comprehensive demonstration of optical control and alignment of bacterial cells, and hierarchical supramolecular organisation utilising dedicated nanocontainer particles.
Content:
Front Matter....Pages i-xi
Motivation and Outline....Pages 1-4
Introduction to Optical Trapping....Pages 5-26
Holographic Phase Contrast....Pages 27-45
Counter-Propagating Traps by Optical Phase-Conjugation....Pages 47-65
Non-Diffracting Beams for the Three-Dimensional Moulding of Matter....Pages 67-82
Ince-Gaussian Beams for the Optical Organisation of Microparticles....Pages 83-94
Holographic Optical Tweezers....Pages 95-116
Summary and Outlook....Pages 117-119
Back Matter....Pages 121-134
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