Ebook: Single Molecule Spectroscopy in Chemistry, Physics and Biology: Nobel Symposium

Written by the leading experts in the field, this book describes the development and current state-of-the-art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.

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Written by the leading experts in the field, this book describes the development and current state-of-the-art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.

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Written by the leading experts in the field, this book describes the development and current state-of-the-art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.

Content:
Front Matter....Pages I-XXI
Front Matter....Pages 1-1
How Biomolecular Motors Work: Synergy Between Single Molecule Experiments and Single Molecule Simulations....Pages 3-22
Front Matter....Pages 24-24
Single-Molecule Optical Spectroscopy and Imaging: From Early Steps to Recent Advances....Pages 25-60
Single Molecules as Optical Probes for Structure and Dynamics....Pages 61-76
FCS and Single Molecule Spectroscopy....Pages 77-103
Front Matter....Pages 106-106
Single Molecule Spectroscopy Illuminating the Molecular Dynamics of Life....Pages 107-117
Chemical Fluxes in Cellular Steady States Measured by Fluorescence Correlation Spectroscopy....Pages 119-137
In Vivo Fluorescence Correlation and Cross-Correlation Spectroscopy....Pages 139-154
Fluorescence Flicker as a Read-out in FCS: Principles, Applications and Further Developments....Pages 155-172
Front Matter....Pages 174-174
Development of Nanocrystal Molecules for Plasmon Rulers and Single Molecule Biological Imaging....Pages 175-186
Size-Minimized Quantum Dots for Molecular and Cellular Imaging....Pages 187-201
Mapping Transcription Factors on Extended DNA: A Single Molecule Approach....Pages 203-216
Front Matter....Pages 218-218
Single Molecule Measurement, a Tool for Exploring the Dynamic Mechanism of Biomolecules....Pages 219-236
Viral DNA Packaging: One Step at a Time....Pages 237-269
Chemo-Mechanical Coupling in the Rotary Molecular Motor F1-ATPase....Pages 271-285
Front Matter....Pages 288-288
Mechanoenzymatics and Nanoassembly of Single Molecules....Pages 289-303
Single Cell Physiology....Pages 305-316
Force-Clamp Spectroscopy of Single Proteins....Pages 317-335
Unraveling the Secrets of Bacterial Adhesion Organelles Using Single-Molecule Force Spectroscopy....Pages 337-362
Front Matter....Pages 364-364
Far-Field Optical Nanoscopy....Pages 365-398
Sub-Diffraction-Limit Imaging with Stochastic Optical Reconstruction Microscopy....Pages 399-415
Front Matter....Pages 364-364
Assessing Biological Samples with Scanning Probes....Pages 417-431
Front Matter....Pages 434-434
Enzymology and Life at the Single Molecule Level....Pages 435-448
Controlling Chemistry in Dynamic Nanoscale Systems....Pages 449-468
Front Matter....Pages 470-470
Single-Molecule Protein Conformational Dynamics in Enzymatic Reactions....Pages 471-494
Watching Individual Enzymes at Work....Pages 495-511
The Influence of Symmetry on the Electronic Structure of the Photosynthetic Pigment-Protein Complexes from Purple Bacteria....Pages 513-533
Front Matter....Pages 536-536
Exploring Nanostructured Systems with Single-Molecule Probes: From Nanoporous Materials to Living Cells....Pages 537-551
Gene Regulation: Single-Molecule Chemical Physics in a Natural Context....Pages 553-560
Back Matter....Pages 561-572

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Written by the leading experts in the field, this book describes the development and current state-of-the-art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.

Content:
Front Matter....Pages I-XXI
Front Matter....Pages 1-1
How Biomolecular Motors Work: Synergy Between Single Molecule Experiments and Single Molecule Simulations....Pages 3-22
Front Matter....Pages 24-24
Single-Molecule Optical Spectroscopy and Imaging: From Early Steps to Recent Advances....Pages 25-60
Single Molecules as Optical Probes for Structure and Dynamics....Pages 61-76
FCS and Single Molecule Spectroscopy....Pages 77-103
Front Matter....Pages 106-106
Single Molecule Spectroscopy Illuminating the Molecular Dynamics of Life....Pages 107-117
Chemical Fluxes in Cellular Steady States Measured by Fluorescence Correlation Spectroscopy....Pages 119-137
In Vivo Fluorescence Correlation and Cross-Correlation Spectroscopy....Pages 139-154
Fluorescence Flicker as a Read-out in FCS: Principles, Applications and Further Developments....Pages 155-172
Front Matter....Pages 174-174
Development of Nanocrystal Molecules for Plasmon Rulers and Single Molecule Biological Imaging....Pages 175-186
Size-Minimized Quantum Dots for Molecular and Cellular Imaging....Pages 187-201
Mapping Transcription Factors on Extended DNA: A Single Molecule Approach....Pages 203-216
Front Matter....Pages 218-218
Single Molecule Measurement, a Tool for Exploring the Dynamic Mechanism of Biomolecules....Pages 219-236
Viral DNA Packaging: One Step at a Time....Pages 237-269
Chemo-Mechanical Coupling in the Rotary Molecular Motor F1-ATPase....Pages 271-285
Front Matter....Pages 288-288
Mechanoenzymatics and Nanoassembly of Single Molecules....Pages 289-303
Single Cell Physiology....Pages 305-316
Force-Clamp Spectroscopy of Single Proteins....Pages 317-335
Unraveling the Secrets of Bacterial Adhesion Organelles Using Single-Molecule Force Spectroscopy....Pages 337-362
Front Matter....Pages 364-364
Far-Field Optical Nanoscopy....Pages 365-398
Sub-Diffraction-Limit Imaging with Stochastic Optical Reconstruction Microscopy....Pages 399-415
Front Matter....Pages 364-364
Assessing Biological Samples with Scanning Probes....Pages 417-431
Front Matter....Pages 434-434
Enzymology and Life at the Single Molecule Level....Pages 435-448
Controlling Chemistry in Dynamic Nanoscale Systems....Pages 449-468
Front Matter....Pages 470-470
Single-Molecule Protein Conformational Dynamics in Enzymatic Reactions....Pages 471-494
Watching Individual Enzymes at Work....Pages 495-511
The Influence of Symmetry on the Electronic Structure of the Photosynthetic Pigment-Protein Complexes from Purple Bacteria....Pages 513-533
Front Matter....Pages 536-536
Exploring Nanostructured Systems with Single-Molecule Probes: From Nanoporous Materials to Living Cells....Pages 537-551
Gene Regulation: Single-Molecule Chemical Physics in a Natural Context....Pages 553-560
Back Matter....Pages 561-572
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