Ebook: Fluorescence Correlation Spectroscopy: Theory and Applications

This book presents the theoretical background to fluorescence correlation spectroscopy (FCS) and a variety of applications in various fields of science. FCS is based on the detection of single molecules excited to fluorescence in diffraction limited confocal volume elements and the time correlation of stochastic events. It provides ultimate sensitivity in the analysis of molecular processes and has found numerous applications in physics, chemistry and particularly in biomolecular sciences. Its high spatial and temporal resolution has made FCS a powerful tool for the analysis of molecular interactions and kinetics, transport properties due to thermal motion and flow, as well as the physics of the excited state in solution as well as at the cellular level. Its application in high throughput drug screening is using all the potential of this prime analytical tool.

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Content:
Front Matter....Pages I-XX
Introduction....Pages 1-6
Front Matter....Pages 7-7
Fluorescence Correlation Spectroscopy of Flavins and Flavoproteins....Pages 9-24
Fluorescence Correlation Spectroscopy in Nucleic Acid Analysis....Pages 25-64
Strain-Dependent Fluorescence Correlation Spectroscopy: Proposing a New Measurement for Conformational Fluctuations of Biological Macromolecules....Pages 65-83
Applications of FCS to Protein-Ligand Interactions: Comparison with Fluorescence Polarization....Pages 84-98
Front Matter....Pages 99-99
FCS-Analysis of Ligand-Receptor Interactions in Living Cells....Pages 101-131
Fluorescence Correlation Microscopy (FCM): Fluorescence Correlation Spectroscopy (FCS) in Cell Biology....Pages 132-161
FCS and Spatial Correlations on Biological Surfaces....Pages 162-184
Front Matter....Pages 185-185
Dual-Color Confocal Fluorescence Spectroscopy and its Application in Biotechnology....Pages 187-203
Nanoparticle Immunoassays: A new Method for Use in Molecular Diagnostics and High Throughput Pharmaceutical Screening based on Fluorescence Correlation Spectroscopy....Pages 204-224
Protein Aggregation Associated with alzhiemer and Prion Diseases....Pages 225-247
Front Matter....Pages 249-249
Application of FCS to the Study of Environmental Systems....Pages 251-275
Photophysical Aspects of FCS Measurements....Pages 276-301
Front Matter....Pages 303-303
Fluorescence Correlation Spectroscopy: Genesis, Evolution, Maturation and Prognosis....Pages 305-330
ConfoCor 2 — The Second Generation of Fluorescence Correlation Microscopes....Pages 331-345
Antibunching and Rotational Diffusion in FCS....Pages 346-359
Cross-correlation analysis in FCS....Pages 360-378
Cross-correlated Flow Analysis in Microstructures....Pages 379-395
Introduction to the Theory of Fluorescence Intensity Distribution Analysis....Pages 396-409
Photon Counting Histogram Statistics....Pages 410-437
Front Matter....Pages 303-303
High Order Autocorrelation in Fluorescence Correlation Spectroscopy....Pages 438-458
FCS in Single Molecule Analysis....Pages 459-476
Back Matter....Pages 477-489

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Content:
Front Matter....Pages I-XX
Introduction....Pages 1-6
Front Matter....Pages 7-7
Fluorescence Correlation Spectroscopy of Flavins and Flavoproteins....Pages 9-24
Fluorescence Correlation Spectroscopy in Nucleic Acid Analysis....Pages 25-64
Strain-Dependent Fluorescence Correlation Spectroscopy: Proposing a New Measurement for Conformational Fluctuations of Biological Macromolecules....Pages 65-83
Applications of FCS to Protein-Ligand Interactions: Comparison with Fluorescence Polarization....Pages 84-98
Front Matter....Pages 99-99
FCS-Analysis of Ligand-Receptor Interactions in Living Cells....Pages 101-131
Fluorescence Correlation Microscopy (FCM): Fluorescence Correlation Spectroscopy (FCS) in Cell Biology....Pages 132-161
FCS and Spatial Correlations on Biological Surfaces....Pages 162-184
Front Matter....Pages 185-185
Dual-Color Confocal Fluorescence Spectroscopy and its Application in Biotechnology....Pages 187-203
Nanoparticle Immunoassays: A new Method for Use in Molecular Diagnostics and High Throughput Pharmaceutical Screening based on Fluorescence Correlation Spectroscopy....Pages 204-224
Protein Aggregation Associated with alzhiemer and Prion Diseases....Pages 225-247
Front Matter....Pages 249-249
Application of FCS to the Study of Environmental Systems....Pages 251-275
Photophysical Aspects of FCS Measurements....Pages 276-301
Front Matter....Pages 303-303
Fluorescence Correlation Spectroscopy: Genesis, Evolution, Maturation and Prognosis....Pages 305-330
ConfoCor 2 — The Second Generation of Fluorescence Correlation Microscopes....Pages 331-345
Antibunching and Rotational Diffusion in FCS....Pages 346-359
Cross-correlation analysis in FCS....Pages 360-378
Cross-correlated Flow Analysis in Microstructures....Pages 379-395
Introduction to the Theory of Fluorescence Intensity Distribution Analysis....Pages 396-409
Photon Counting Histogram Statistics....Pages 410-437
Front Matter....Pages 303-303
High Order Autocorrelation in Fluorescence Correlation Spectroscopy....Pages 438-458
FCS in Single Molecule Analysis....Pages 459-476
Back Matter....Pages 477-489
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