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Biophotonics - Optical Science and Engineering for the 21st Century

- Roeland Van Wijk, PhD. and Prof. Dr. Xun Shen

Biophotonics, the science of generating and harnessing light photons to image, detect and manipulate biological materials, offers great hope for the early detection of diseases and for new modalities of light-guided and light-activated therapies. It offers a powerful tool for studying molecular events, such as gene expression, protein-protein interaction, spatial and temporal distribution of the molecules of biological interest, and many chemico-physical processes in living cells and living organisms.

Biophotonics - Optical Science and Engineering for the 21st Century introduces the basic experimental and theoretical framework, the technical problems, and the wide field of applications in biotechnology, biomedical engineering, engineering, medicine, pharmacology, environmental science, life sciences, and clinical sciences. Biophotonics: Optical Science and Engineering in the 21st Century serves as an ideal aid to the research and development of these areas integrating light, photonics, and biological systems.

Key topics include:

Fluctuation Correlation Spectroscopy in Cells: Determination of Molecular Aggregation

Using GFP and FRET Technologies for Studying Signaling Mechanisms of Apoptosis in a Single Living Cell

Study on Protein-Protein Interaction in Single Living Cells

Functional Optical Coherence Tomography: Simultaneous In Vivo Imaging of Tissue Structure and Physiology

Imaging –Photo- and Sonodynamic Diagnosis of Cancer Mediated by Chemiluminescence Probes

Biophotonic Analysis of Spontaneous Self-Organizing Oxidative Processes in Aqueous Systems

Biophoton Emission and Defense Systems in Plants

Biophotonics - Optical Science and Engineering for the 21st Century stands as a valuable resource for advanced undergraduate, graduate students and researchers in biomedical engineering, biotechnology, engineering, chemistry, physics, life sciences, and clinical sciences.

Editors:

Xun Shen

Professor, Institute of Biophysics, Chinese Academy of Sciences

Beijing, China

Roeland Van Wijk

Professor, Department of Molecular Cell Biology, Utrecht University,

Utrecht, The Netherlands





Biophotonics - Optical Science and Engineering for the 21st Century

- Roeland Van Wijk, PhD. and Prof. Dr. Xun Shen 

Biophotonics, the science of generating and harnessing light photons to image, detect and manipulate biological materials, offers great hope for the early detection of diseases and for new modalities of light-guided and light-activated therapies. It offers a powerful tool for studying molecular events, such as gene expression, protein-protein interaction, spatial and temporal distribution of the molecules of biological interest, and many chemico-physical processes in living cells and living organisms.

Biophotonics - Optical Science and Engineering for the 21st Century introduces the basic experimental and theoretical framework, the technical problems, and the wide field of applications in biotechnology, biomedical engineering, engineering, medicine, pharmacology, environmental science, life sciences, and clinical sciences. Biophotonics: Optical Science and Engineering in the 21st Century serves as an ideal aid to the research and development of these areas integrating light, photonics, and biological systems.

Key topics include:

Fluctuation Correlation Spectroscopy in Cells: Determination of Molecular Aggregation

Using GFP and FRET Technologies for Studying Signaling Mechanisms of Apoptosis in a Single Living Cell

Study on Protein-Protein Interaction in Single Living Cells

Functional Optical Coherence Tomography: Simultaneous In Vivo Imaging of Tissue Structure and Physiology

Imaging –Photo- and Sonodynamic Diagnosis of Cancer Mediated by Chemiluminescence Probes

Biophotonic Analysis of Spontaneous Self-Organizing Oxidative Processes in Aqueous Systems

Biophoton Emission and Defense Systems in Plants

Biophotonics - Optical Science and Engineering for the 21st Century stands as a valuable resource for advanced undergraduate, graduate students and researchers in biomedical engineering, biotechnology, engineering, chemistry, physics, life sciences, and clinical sciences.

Editors:

Xun Shen

Professor, Institute of Biophysics, Chinese Academy of Sciences

Beijing, China

Roeland Van Wijk

Professor, Department of Molecular Cell Biology, Utrecht University,

Utrecht, The Netherlands





Biophotonics - Optical Science and Engineering for the 21st Century

- Roeland Van Wijk, PhD. and Prof. Dr. Xun Shen 

Biophotonics, the science of generating and harnessing light photons to image, detect and manipulate biological materials, offers great hope for the early detection of diseases and for new modalities of light-guided and light-activated therapies. It offers a powerful tool for studying molecular events, such as gene expression, protein-protein interaction, spatial and temporal distribution of the molecules of biological interest, and many chemico-physical processes in living cells and living organisms.

Biophotonics - Optical Science and Engineering for the 21st Century introduces the basic experimental and theoretical framework, the technical problems, and the wide field of applications in biotechnology, biomedical engineering, engineering, medicine, pharmacology, environmental science, life sciences, and clinical sciences. Biophotonics: Optical Science and Engineering in the 21st Century serves as an ideal aid to the research and development of these areas integrating light, photonics, and biological systems.

Key topics include:

Fluctuation Correlation Spectroscopy in Cells: Determination of Molecular Aggregation

Using GFP and FRET Technologies for Studying Signaling Mechanisms of Apoptosis in a Single Living Cell

Study on Protein-Protein Interaction in Single Living Cells

Functional Optical Coherence Tomography: Simultaneous In Vivo Imaging of Tissue Structure and Physiology

Imaging –Photo- and Sonodynamic Diagnosis of Cancer Mediated by Chemiluminescence Probes

Biophotonic Analysis of Spontaneous Self-Organizing Oxidative Processes in Aqueous Systems

Biophoton Emission and Defense Systems in Plants

Biophotonics - Optical Science and Engineering for the 21st Century stands as a valuable resource for advanced undergraduate, graduate students and researchers in biomedical engineering, biotechnology, engineering, chemistry, physics, life sciences, and clinical sciences.

Editors:

Xun Shen

Professor, Institute of Biophysics, Chinese Academy of Sciences

Beijing, China

Roeland Van Wijk

Professor, Department of Molecular Cell Biology, Utrecht University,

Utrecht, The Netherlands



Content:
Front Matter....Pages i-xviii
Fluctuation Correlation Spectroscopy in Cells:....Pages 1-14
Dynamics of the Cell Membrane Observed under the Evanescent Wave Microscope and the Confocal Microscope....Pages 15-23
Using GFP and FRET Technologies for Studying Signaling Mechanisms of Apoptosis in a Single Living Cell....Pages 25-38
Fluorescence Resonance Energy Transfer (FRET) Study on Protein-Protein Interaction in Single Living Cells....Pages 39-52
Functional Optical Coherence Tomography: Simultaneous in Vivo Imaging of Tissue Structure and Physiology....Pages 53-71
Temporal Clustering Analysis of Cerebral Blood Flow Activation Maps Measured by Laser Speckle Contrast Imaging....Pages 73-84
Photo- and Sono-Dynamic Diagnosis of Cancer Mediated by Chemiluminescence Probes....Pages 85-97
Bioluminescence Assay for the Human Chaperone MRJ Facilitated Refolding of Luciferase in Vitro....Pages 99-107
Essential Differences Between Coherent and Non-Coherent Effects of Photon Emission from Living Organisms....Pages 109-124
Parameters Characterizing Spontaneous Biophoton Signal as a Squeezed State in a Sample of Parmelia.Tinctorum ....Pages 125-140
Biophotonic Analysis of Spontaneous Self-Organizing Oxidative Processes in Aqueous Systems....Pages 141-154
Two-Dimensional Imaging and Spatiotemporal Analysis of Biophoton....Pages 155-171
Ultraweak Photon Emission from Human Body....Pages 173-184
Laser-Ultraviolet-A Induced Biophotonic Emission in Cultured Mammalian Cells....Pages 185-194
Biophoton Emission and Delayed Luminescence of Plants....Pages 195-204
Biophoton Eemission and Defense Systems in Plants....Pages 205-218
Back Matter....Pages 219-222


Biophotonics - Optical Science and Engineering for the 21st Century

- Roeland Van Wijk, PhD. and Prof. Dr. Xun Shen 

Biophotonics, the science of generating and harnessing light photons to image, detect and manipulate biological materials, offers great hope for the early detection of diseases and for new modalities of light-guided and light-activated therapies. It offers a powerful tool for studying molecular events, such as gene expression, protein-protein interaction, spatial and temporal distribution of the molecules of biological interest, and many chemico-physical processes in living cells and living organisms.

Biophotonics - Optical Science and Engineering for the 21st Century introduces the basic experimental and theoretical framework, the technical problems, and the wide field of applications in biotechnology, biomedical engineering, engineering, medicine, pharmacology, environmental science, life sciences, and clinical sciences. Biophotonics: Optical Science and Engineering in the 21st Century serves as an ideal aid to the research and development of these areas integrating light, photonics, and biological systems.

Key topics include:

Fluctuation Correlation Spectroscopy in Cells: Determination of Molecular Aggregation

Using GFP and FRET Technologies for Studying Signaling Mechanisms of Apoptosis in a Single Living Cell

Study on Protein-Protein Interaction in Single Living Cells

Functional Optical Coherence Tomography: Simultaneous In Vivo Imaging of Tissue Structure and Physiology

Imaging –Photo- and Sonodynamic Diagnosis of Cancer Mediated by Chemiluminescence Probes

Biophotonic Analysis of Spontaneous Self-Organizing Oxidative Processes in Aqueous Systems

Biophoton Emission and Defense Systems in Plants

Biophotonics - Optical Science and Engineering for the 21st Century stands as a valuable resource for advanced undergraduate, graduate students and researchers in biomedical engineering, biotechnology, engineering, chemistry, physics, life sciences, and clinical sciences.

Editors:

Xun Shen

Professor, Institute of Biophysics, Chinese Academy of Sciences

Beijing, China

Roeland Van Wijk

Professor, Department of Molecular Cell Biology, Utrecht University,

Utrecht, The Netherlands



Content:
Front Matter....Pages i-xviii
Fluctuation Correlation Spectroscopy in Cells:....Pages 1-14
Dynamics of the Cell Membrane Observed under the Evanescent Wave Microscope and the Confocal Microscope....Pages 15-23
Using GFP and FRET Technologies for Studying Signaling Mechanisms of Apoptosis in a Single Living Cell....Pages 25-38
Fluorescence Resonance Energy Transfer (FRET) Study on Protein-Protein Interaction in Single Living Cells....Pages 39-52
Functional Optical Coherence Tomography: Simultaneous in Vivo Imaging of Tissue Structure and Physiology....Pages 53-71
Temporal Clustering Analysis of Cerebral Blood Flow Activation Maps Measured by Laser Speckle Contrast Imaging....Pages 73-84
Photo- and Sono-Dynamic Diagnosis of Cancer Mediated by Chemiluminescence Probes....Pages 85-97
Bioluminescence Assay for the Human Chaperone MRJ Facilitated Refolding of Luciferase in Vitro....Pages 99-107
Essential Differences Between Coherent and Non-Coherent Effects of Photon Emission from Living Organisms....Pages 109-124
Parameters Characterizing Spontaneous Biophoton Signal as a Squeezed State in a Sample of Parmelia.Tinctorum ....Pages 125-140
Biophotonic Analysis of Spontaneous Self-Organizing Oxidative Processes in Aqueous Systems....Pages 141-154
Two-Dimensional Imaging and Spatiotemporal Analysis of Biophoton....Pages 155-171
Ultraweak Photon Emission from Human Body....Pages 173-184
Laser-Ultraviolet-A Induced Biophotonic Emission in Cultured Mammalian Cells....Pages 185-194
Biophoton Emission and Delayed Luminescence of Plants....Pages 195-204
Biophoton Eemission and Defense Systems in Plants....Pages 205-218
Back Matter....Pages 219-222
....
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