Ebook: Mechanisms of Work Production and Work Absorption in Muscle

`In contrast to common practice, we have always tried to include as many discussions held at the meeting in our proceedings as possible, so as to enable readers to properly evaluate each paper presented, as well as to learn of future prospects in this field of research. Although the policy of including discussions occasions a long publication delay, we believe that it is worth repeating in our future publication, as we have met a number of young investigators fascinated by the discussions in our proceedings....
In the concluding remarks in this volume, Dr. Hugh E. Huxley, a principal architect of the sliding filament mechanism of muscle contraction, states that the molecular mechanism of myofilament sliding remains mysterious to all of us. We hope that this volume will stimulate muscle investigators to design and perform novel experiments to clarify the mysteries in muscle contraction.'
Haruo Sugi and Gerald H. Pollack, excerpted from the Preface.

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`In contrast to common practice, we have always tried to include as many discussions held at the meeting in our proceedings as possible, so as to enable readers to properly evaluate each paper presented, as well as to learn of future prospects in this field of research. Although the policy of including discussions occasions a long publication delay, we believe that it is worth repeating in our future publication, as we have met a number of young investigators fascinated by the discussions in our proceedings....
In the concluding remarks in this volume, Dr. Hugh E. Huxley, a principal architect of the sliding filament mechanism of muscle contraction, states that the molecular mechanism of myofilament sliding remains mysterious to all of us. We hope that this volume will stimulate muscle investigators to design and perform novel experiments to clarify the mysteries in muscle contraction.'
Haruo Sugi and Gerald H. Pollack, excerpted from the Preface.

---

`In contrast to common practice, we have always tried to include as many discussions held at the meeting in our proceedings as possible, so as to enable readers to properly evaluate each paper presented, as well as to learn of future prospects in this field of research. Although the policy of including discussions occasions a long publication delay, we believe that it is worth repeating in our future publication, as we have met a number of young investigators fascinated by the discussions in our proceedings....
In the concluding remarks in this volume, Dr. Hugh E. Huxley, a principal architect of the sliding filament mechanism of muscle contraction, states that the molecular mechanism of myofilament sliding remains mysterious to all of us. We hope that this volume will stimulate muscle investigators to design and perform novel experiments to clarify the mysteries in muscle contraction.'
Haruo Sugi and Gerald H. Pollack, excerpted from the Preface.
Content:
Front Matter....Pages i-xv
Introductory Remarks....Pages 1-4
Linear and Rotary Molecular Motors....Pages 5-14
Measurement of ATPase Activities of Myosin at the Level of Tracks and Single Molecules....Pages 15-27
Evanescent Excitation Microscopy....Pages 29-36
Three-Dimensional Image Analysis of Myosin Head in Function as Captured by Quick-Freeze Deep-Etch Replica Electron Microscopy....Pages 37-45
Fluctuation Correlation in the Sliding Movement Generated by Protein Motors In Vitro....Pages 47-51
ATP-Induced Axial Movement of Myosin Heads in Living Thick Filaments Recorded with a Gas Environmental Chamber attached to the Electron Microscope....Pages 53-62
Design Principles and Applications of a Cooled CCD Camera for Electron Microscopy....Pages 63-72
Structure of the Monomeric Actin-Myosin Head Complex as Revealed by X-Ray Solution Scattering....Pages 73-78
The Use of Fast Kinetic Systems for the Study of ng-?g Quantities of Purified Muscle Proteins....Pages 79-89
Inhibitory Actions of Synthetic Actin Peptides and 2,3-Butanedion Monoxime on Actomyosin System....Pages 91-98
Smooth Muscle Myosin....Pages 99-104
The Molecular Biology and Pathophysiology of Hypertrophic Cardiomyopathy Due to Mutations in the Beta Myosin Heavy Chains and the Essential and Regulatory Light Chains....Pages 105-115
Cross-Bridge Dynamics in the Contracting Heart....Pages 117-124
Distinct Kinetic Properties of Cardiac Myosin Isoforms Revealed by In Vitro Studies....Pages 125-130
Functional Characterization of Dictyostelium Discoideum Mutant Myosins Equivalent to Human Familial Hypertrophic Cardiomyopathy....Pages 131-137
Hydrodynamic Properties of Water in Contractile System....Pages 139-144
The Crystal Structure of Troponin C in Complex with N-Terminal Fragment of Troponin I....Pages 145-155
Molecular Switches in Troponin....Pages 157-167
Fluorescence of NBD-Labelled Troponin-I as a Probe for the Kinetics of Thin Filament Activation in Skeletal Muscle Fibers....Pages 169-176
Skeletal Muscle Regulatory Proteins Enhance F-Actin In Vitro Motility....Pages 177-185
Signal Transmission and Transduction in Excitation-Contraction Coupling....Pages 187-197
Regulation of Contraction in Heart Muscle....Pages 199-207
Role of Calcium and Crossbridges in Modulation of Rates of Force Development and Relaxation in Skinned Muscle Fibers....Pages 209-218
Structure and Function of Smooth Muscle Myosin Light Chain Kinase....Pages 219-228
Regulation of Scallop Myosin by Calcium....Pages 229-234
Modulatory Effect of MgATP on Smooth Muscle Myosin Phosphatase Activity....Pages 235-240
Studies on the 14.5 nm Meridional X-Ray Diffraction Reflection During Length Changes of Intact Frog Muscle Fibres....Pages 241-246
On the Working Stroke Elicited by Steps in Length and Temperature....Pages 247-258
Myosin Head Movements during Isometric Contraction Studied by X-Ray Diffraction of Single Frog Muscle Fibres....Pages 259-264
X-Ray Diffraction Studies of the Cross-Bridge Intermediate States....Pages 265-270
Spacing Changes in the Actin and Myosin Filaments during Activation, and Their Implications....Pages 271-279
Structural Features of Force-Generating Cross-Bridges....Pages 281-288
Myosin Crossbridge Configurations in Equilibrium States of Vertebrate Skeletal Muscle....Pages 289-296
Extensibility of the Actin and Myosin Filaments in Various States of Skeletal Muscle as Studied by X-Ray Diffraction....Pages 297-308
Contractile Properties of Thin (Actin) Filament-Reconstituted Muscle Fibers....Pages 309-317
The ATPase Activity in Isometric and Shortening Skeletal Muscle Fibres....Pages 319-329
Force Generation Simplified....Pages 331-341
Evidence for Two Distinct Cross-Bridge Populations in Tetanized Frog Muscle Fibers Stretched with Moderate Velocities....Pages 343-352
Implications of Quantal Motor Action in Biological Systems....Pages 353-359
Calcium Dependence of the Apparent Rate of Force Generation in Single Striated Muscle Myofibrils Activated by Rapid Solution Changes....Pages 361-371
Myofilament Compliance and Sarcomere Tension-Stiffness Relation during the Tetanus Rise in Frog Muscle Fibres....Pages 373-382
Muscle Work Enhancement by Stretch....Pages 383-392
The Physiological Evaluation of Gene Therapies of Dystrophin-Deficient Muscles....Pages 393-409
Communications Between the Nuclotide-and Actin-Binding Site of the Myosin Head in Muscle Fibers....Pages 411-417
Crossbridge Head Detachment Rate Constants Determined from a Model that Explains the Behavior of Both Weakly-and Strongly-Binding Crossbridges....Pages 419-423
Bidirectional Functional Communication between Myosin Subfragments 1 and 2 in Skeletal Muscle Fibers....Pages 425-434
Reappraisal of the Multicellular Preparation for the In Vitro Physiopharmacological Evaluation of Myocardial Performance....Pages 435-440
Alterations of Myocardial Contraction Associated with a Structural Heart Defect in Embryonic Chicks....Pages 441-451
Influences of Protein Kinase A and D-Camp on Actin-Myosin Interaction and Energy Consumption of Cardiac Muscles....Pages 453-459
Work Production and Work Absorption in Muscle Strips from Vertebrate Cardiac and Insect Flight Muscle Fibers....Pages 461-470
Expression and Function of Atrial Myosin Light Chain1 in the Porcine Right Ventricle of Normal and Pulmonary Hypertensive animals....Pages 471-480
Ventricular Pressure-Volume Area (PVA) Accounts for Cardiac Energy Consumption of Work Production and Absorption....Pages 481-489
Oxygen Consumption and Motility of Mechanically Unloaded Myocardial Slices....Pages 491-498
Vascular Endothelial Cell-Cardiac Myocyte Crosstalk in Achieving a Balance between Energy Supply and Energy use....Pages 499-506
Ramp-Rate Dependent External Work During Ramp-Load Release in Cardiac Muscle....Pages 507-514
Efficiency of Skeletal and Cardiac Muscle....Pages 515-526
Muscle Energetics during Unfused Tetanic Contractions....Pages 527-536
Shortening during Stimulation vs. during Relaxation....Pages 537-544
Load-Dependent Mechanical Efficiency of Individual Myosin Heads in Skeletal Muscle Fibers Activated by Laser Flash Photolysis of Caged Calcium in the Presence of a Limited amount of ATP....Pages 545-555
Measurement of ATP Turnover during Shortening and Lengthening of Rabbit Psoas Myofibrils Using a Fluorescent ATP analog....Pages 557-567
Concluding Remarks....Pages 569-646
Back Matter....Pages 647-653
....Pages 655-663