Ebook: Models for Assessing Drug Absorption and Metabolism

Pharmaceutical scientists in industry and academia will appreciate this single reference for its detailed experimental procedures for conducting biopharmaceutical studies. This well-illustrated guide allows them to establish, validate, and implement commonly used in situ and in vitro model systems. Chapters provide ready access to these methodologies for studies of the intestinal, buccal, nasal and respiratory, vaginal, ocular, and dermal epithelium as well as the endothelial and elimination barriers.

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Content:
Front Matter....Pages i-xxii
General Principles in the Characterization and Use of Model Systems for Biopharmaceutical Studies....Pages 1-11
Cultured Intestinal Epithelial Cell Models....Pages 13-34
Intestinal Rings and Isolated Intestinal Mucosal Cells....Pages 35-50
Models of Drug Absorption in Situ and in Conscious Animals....Pages 51-66
Model Systems for Intestinal Lymphatic Transport Studies....Pages 67-84
Buccal Tissues and Cell Culture....Pages 85-102
Isolated Hepatocytes....Pages 103-113
Cultured Rat Hepatocytes....Pages 115-120
Isolated Perfused Liver....Pages 121-159
Isolated Renal Brush Border and Basolateral Membrane Vesicles and Cultured Renal Cells....Pages 161-192
Use of an Isolated Perfused Kidney to Assess Renal Clearance of Drugs....Pages 193-210
Brain Microvessel Endothelial Cell Culture Systems....Pages 211-238
Methods to Study Drug Transport in Isolated Choroid Plexus Tissue and Cultured Cells....Pages 239-258
Brain Perfusion Systems for Studies of Drug Uptake and Metabolism in the Central Nervous System....Pages 259-283
Models for Investigation of Peptide and Protein Transport across Cultured Mammalian Respiratory Epithelial Barriers....Pages 285-307
Isolated Perfused Porcine Skin Flap Systems....Pages 309-323
Vaginal Epithelial Models....Pages 325-346
Ocular Epithelial Models....Pages 347-359
Back Matter....Pages 361-374
....Pages 375-386