Ebook: Protein Purification. From Molecular Mechanisms to Large-Scale Processes

Content: Large-scale protein purification : introduction / Richard C. Willson and Michael R. Ladisch --
Strategies for large-scale protein purification / Sa V. Ho --
Separations in biotechnology : the key role of adsorptive separations / E.N. Lightfoot --
Peptide and protein partitioning in aqueous two-phase systems : effect of amino acid sequence / Alan D. Diamond, Kun Yu, and James T. Hsu --
Protein separation via polyelectrolyte complexation / Mark A. Strege, Paul L. Dubin, Jeffrey S. West, and C. Daniel Flinta --
Mechanisms of protein retention in hydrophobic interaction chromatography / Belinda F. Roettger, Julia A. Myers, Michael R. Ladisch, and Fred E. Regnier --
Anion exchange stationary phase for [beta]-galactosidase, bovine serum albumin, and insulin : separation and sorption characteristics / Michael R. Ladisch, Richard L. Hendrickson, and Karen L. Kohlmann --
Radial-flow affinity chromatography for trypsin purification / Wen-Chien Lee, Gow-Jen Tsai, and George T. Tsao --
Impact of continuous affinity-recycle extraction (CARE) in downstream processing / Neal F. Gordon and Charles L. Cooney --
Novel metal-affinity protein separations / S.-S. Suh, M.E. Van Dam, G.E. Wuenschell, S. Plunkett, and F.H. Arnold --
Recovery of recombinant proteins by immunoaffinity chromatography / Pascal Bailon and Swapan K. Roy --
Chelating peptide-immobilized metal-ion affinity chromatography / Michele C. Smith, James A. Cook, Thomas C. Furman, Paul D. Gesellchen, Dennis P. Smith, and Hansen Hsiung --
Site-specific proteolysis of fusion proteins / Paul Carter --
Purification alternatives for IgM (human) monoclonal antibodies / G.B. Dove, G. Mitra, G. Roldan, M.A. Shearer, and M.-S. Cho --
Analytical, preparative, and large-scale zone electrophoresis / Cornelius F. Ivory, William A. Gobie, and Tri P. Adhi --
Applied electric fields for downstream processing / Scott R. Rudge and Paul Todd.