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In addition to supplementing existing analysis techniques in serious crime cases, trace DNA can allow investigation of volume crime cases such as burglary or vehicle theft, where previously DNA evidence was not considered usable. However, despite the widespread use of trace DNA, at present there are very few specific validated methods. This has lead to controversy in the use of trace DNA, and particularly the low copy number amplification technique. It has been established that the use of existing methodology (developed for high-copy number samples) leads to significant levels of artefacts with trace DNA, including allele drop-out and drop-in, stutter, and allelic/locus imbalance. To minimize these, there are numerous modifications that can be made to existed methods to increase the success of trace DNA analysis. This book presents advances in the field of DNA research as an aid in Forensic studies and Genetics.
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