Ebook: DNA Repair Protocols: Mammalian Systems

In this second edition of a much praised laboratory manual devoted to eukaryotic systems, Daryl S. Henderson has refocused the book on mammalian cells, adding fourteen entirely new chapters and extensively revising many of the remaining chapters. The authors address a broad range of questions about practical mammalian DNA repair, including such arcana as "what is radioresistant DNA synthesis and how is it measured?" The techniques presented are readily reproducible and offer cutting-edge methods for cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of "adducted" bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, DNA Repair Protocols: Mammalian Systems, Second Edition, offers investigators a wide variety of productive methods to explore and make new discoveries in the world of mammalian DNA repair.

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Personally I think "DNA Repair Protocol: Eukaryotic Systems" is a great academic book based on my over ten years of research experiences in Genetic Toxicology. This book provides detailed, step-by-step instructions for studying manifold aspects of the eukaryotic responses to genotoxic damage, including a lot of useful tips in handling all kinds of DNA repair experiments. Compared with Friedberg's textbook "DNA Repair and Mutagenesis", which comprehensively covers all fundamental knowledge about DNA damage, mutagenesis and DNA repair, this book focused on detailed experimental protocols and new techniques, plus a wonderful and concise introduction and explanation of each methods. In my opinion, this book refreshments my background knowledge, gives me an overview of recent progress in new methods and techniques, and ignites some new ideas to pursue further studies in this active field. Taken together, I think this is an essential handbook for professors and scientists in DNA damage and repair field and other related fields.