Ebook: Gel Electrophoresis of Proteins

Front Cover -- Gel Electrophoresis of Proteins -- Copyright Page -- Table of Contents -- Preface -- List of Contributors -- List of Abbreviations -- Chapter 1. Steady-state Gel Electrophoresis Systems -- 1.1 Introduction -- 1.2 Historical Developments -- 1.3 Fundamental Steady-state Electrophoresis Systems -- 1.4 Fundamental Properties of Steady-state Electrophoresis Systems -- 1.5 Nomenclature and Definitions -- 1.6 Conductivity -- 1.7 Equations for Calculation of System Composition -- 1.8 Isotachophoresis -- 1.9 Moving Boundary Electrophoresis -- 1.10 Isoelectric Focusing Systems -- Acknowledgements -- References -- Chapter 2. One-dimensional PAA-gel Electrophoretic Techniques to Separate Functional and Denatured Proteins -- 2.1 Introduction -- 2.2 Structure and Physico-chemical Properties of Polyacrylamide Gels -- 2.3 Analytical Polyacrylamide Gel Electrophoresis -- 2.4 Affinity Electrophoresis -- Acknowledgments -- References -- Chapter 3. Conventional Isoelectric Focusing and Immobilised pH Gradients -- 3.1 Introduction -- 3.2 The Principles of IEF -- 3.3 IEF in Agarose Matrices -- 3.4 IEF in Polyacrylamide Matrices -- 3.5 Titration Curves -- 3.6 Production of Narrow pH Gradients -- 3.7 Measurements of pH Gradients -- 3.8 Trouble Shooting -- 3.9 Artefacts: a Unified View -- 3.10 The Chemicals -- the Immobiline Matrix -- 3.11 Narrow and Ultra-narrow pH Gradients -- 3.12 Extended pH Gradients -- 3.13 On Buffering Capacity and Ionic Strength -- 3.14 On Electro-endosmosis -- 3.15 Polymerisation Kinetics -- 3.16 Methodology: Casting an Immobiline Gel -- 3.17 Artefacts -- 3.18 Conclusions -- Acknowledgments -- References -- Chapter 4. High Resolution Two-dimensional Polyacrylamide-gel Electrophoresis -- 4.1 Introduction -- 4.2 Two-dimensional Electrophoresis under Non-denaturing Conditions.;Gel Electrophoresis of Proteins focuses on the techniques, methodologies, reactions, and approaches involved in gel electrophoresis of proteins. The selection first covers steady-state gel electrophoresis systems and one-dimensional PAA-gel electrophoretic techniques to separate functional and denatured proteins. Discussions focus on affinity electrophoresis, structure and physico-chemical properties of polyacrylamide gels, moving boundary electrophoresis, isotachophoresis, fundamental steady-state electrophoresis systems, and fundamental properties of steady-state electrophoresis systems. The text then reviews conventional isoelectric focusing and immobilized pH gradients and high resolution two-dimensional polyacrylamide-gel electrophoresis. Topics include production of narrow pH gradients, extended pH gradients, polymerization kinetics, IEF in agarose matrices, titration curves, two-dimensional electrophoresis under non-denaturing conditions, and IEF in polyacrylamide matrices. The book tackles quantifying patterns from two-dimensional PAGE, protein staining and detection methods, and immunoelectrophoretic methods. Concerns include post-electrophoretic organic protein stains, silver and enzyme stains, detection of radioactive proteins, and programming languages. The selection is highly recommended for researchers wanting to conduct studies on gel electrophoresis of proteins.