Ebook: Circadian Rhythms: Methods and Protocols

The aim of Circadian Rhythms is to provide a resource that can be adopted by several types of users: those who are new to circadian biology, those who are already active in the field but are interested in learning new techniques and researchers who are considering moving to a new a model system or undertaking comparative studies and would like to consult protocols applied to different organisms before starting the study of new species. This book features a full range of methods that illustrate procedures that have been recently been introduced in circadian studies and by presenting variations to take into account the peculiarities of different model systems.

Circadian Rhythms: Methods and Protocols is an in-depth examination of circadian biology, presented by leading researchers in the field. This cutting-edge collection of protocols is adaptable for research at every level, and represents the huge strides that chronobiologists have made over the past two decades. These robust, reproducible procedures take into account the myriad of variables that circadian researchers must be cognizant of while conducting their research. By distilling the arsenal of methodologies available, and presenting essential procedures in precise detail, Circadian Rhythms: Methods and Protocols supports a vast majority of circadian research efforts.

Part I of this seven-part volume provides a concise introduction to essential components of circadian biology, including light, photoreceptors, circadian clocks, and statistical analysis. Part II presents protocols and techniques for monitoring and analyzing rhythmic patterns of several model organisms. Parts III, IV, and V emphasize the identification of clock genes and protein and RNA expression in circadian biology. Protocols for in vitro systems comprise Part VI, and Part VII provides an outline of methods and tips for microscopy analysis. Circadian biologists at all research levels will realize tremendous benefit from this extraordinary collection of methods and protocols for the study of circadian rhythms.