Ebook: Gel Electrophoresis - Principles and Basics

InTech, 2012, 366 pages, ISBN: 9535104582 9789535104582In this book, the authors try to present simplified fundamentals of gel-based separation together with exemplarily applications of this versatile technique. The contents of the book crisp and comprehensive, it will receive overwhelming interest and deliver benefits and valuable information to the readers.Contents

Basic Principles of Gel Electrophoresis
Introduction to Agarose and Polyacrylamide Gel Electrophoresis Matrices with Respect to Their Detection Sensitivities
Gel-Electrophoresis and Its Applications
Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis
Discriminatory Power of Agarose Gel Electrophoresis in DNA Fragments Analysis
Gel Electrophoresis of Proteins
Gel Electrophoresis of Protein - From Basic Science to Practical Approach

Two Dimensional Polyacrylamide Gel Electrophoresis
Two-Dimensional Polyacrylamide Gel Electrophoresis - A Practical Perspective
High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis: ATool for Identification of Polymorphic and Modified Linker Histone Components
Two-Dimensional Gel Electrophoresis (2-DE)
High Speed Isoelectric Focusing of Proteins Enabling Rapid Two-Dimensional Gel Electrophoresis

Denaturing Gradient Gel Electrophoresis (DGGE)
Denaturing Gradient Gel Electrophoresis (DGGE) in Microbial Ecology - Insights from Freshwaters

Statistical and Bioinformatic Analysis of Electrophoresis Data
Statistical Analysis of Gel Electrophoresis Data
Quantitative Analysis of Electrophoresis Data - Application to Sequence-Specific Ultrasonic Cleavage of DNA

Pulsed Field Gel Electrophoresis (PFGE)
The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing - Harmonization at the European Union Level

Bacterial Electrophoretic Techniques
Electrophoretic Techniques in Microbial Ecology
Application of Multiplex PCR, Pulsed-Field Gel Electrophoresis (PFGE), and BOX-PCRfor Molecular Analysis of Enterococci
The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing - Comparison with MLVA Method Coupled with Gel Electrophoresis
Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Fragments (PCR-RFLP) and Gel Electrophoresis - Valuable Tool for Genotyping and Genetic Fingerprinting
Application of Two-Dimensional Gel Electrophoresis to Microbial Systems