Ebook: Isolation of Lepidopteran Active Native Bacillus thuringiensis Strains Through PCR Panning

Статья. Опубликована в Asia Pacific Journal of Molecular Biology and Biotechnology, 2007. Vol. 15 (2). P 61-67
Screening the environment for new and highly potent strains of Bacillus thuringiensis (B. thuringiensis) has become inevitable as one of the strategies for insect resistance management. By adopting the modified acetate selection method, entomocidally potent B. thuringiensis isolates were obtained from grain samples and soil samples from sericulture environment. PCR was performed to determine the insecticidal potential of the isolates. SDS-PAGE analysis of PCR positive isolates exhibited typical Cry1 protein profiles with 130 to 140 kDa protoxin. Preliminary larvicidal assays against Heliothis armigera with spore-crystal mixture, showed that all 30 B. thuringiensis isolates were toxic to this species.