Ebook: Biomedical Tissue Culture

InTech, 2012, 238 pages, ISBN: 9535107880 9789535107880This book describes many aspects of tissue culture models in an extensive manner.The book includes many topics like, the development of cultural methods to produce massive neuronal syncytial connections and induce their fusion with formation of bi- and multinucleated cells, the applicability in research of cell lines derived from the cultivation of placenta derived cells and cell populations with properties of progenitor/stem cells, the procedures that may be used to regenerate cartilage tissue with appropriate mechanical properties, the improvements made in the use of cell culture for virus isolation, tissue-based models for HCV replication in vitro, cultures to study the pathogenicity of enteropathogenic bacteria, the use of viral DNA and cDNA Array in the diagnosis of respiratory tract infections (RTI) in comparison with routine diagnosis methods.ContentsGeneral Characteristics and Culture Conditions
Culture Conditions and Types of Growth Media for Mammalian Cells
Cell Handling and Culture Under Controlled Oxygen Concentration
Contamination of Tissue Cultures by Mycoplasmas
Isolation of Breast Cancer Stem Cells by Single-Cell Sorting
In vitro, Tissue-Based Models as a Replacement for Animal Models in Testing of Drugs at the Preclinical StagesSpecific Uses of Tissue Culture
Use of Cell Culture to Prove Syncytial Connection and Fusion of Neurons
Placental Structure and Biological Aspects of Fetal Membranes Cultured in vitro
Tissue Development and Mechanical Property in the Regenerated-Cartilage Tissue
The Art of Animal Cell Culture for Virus Isolation
Tissue-Based Model of HCV Replication as a Replacement for Animal Models in Drug Testing
Tissue Culture to Assess Bacterial Enteropathogenicity
Viral DNA and cDNA Array in the Diagnosis of Respiratory Tract Infections